Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx
Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx
... Access
Production and purification of VP2 protein of
porcine parvovirus expressed in an insect-
baculovirus cell system
Hongchao Zhou
1†
, Guizhe Yao
2†
, Shangjin Cui
2*†
Abstract
The porcine parvovirus ... costly than bacterial
expression systems for producing viral proteins and may
present difficulty in purifying expressed proteins from
insect cell an...
Báo cáo Y học: Production and chemiluminescent free radical reactions of glyoxal in lipid peroxidation of linoleic acid by the ligninolytic enzyme, manganese peroxidase pot
... 0) and
the enzyme solution. Reactions were started by adding H
2
O
2
and were quantified by monitoring the initial rate of
increase in absorbance at 470 nm in the presence and
absence of manganese. ... (a) singlet oxygen, (b) triplet carbonyls
from dioxetane and a-hydroxyperoxyl radicals, and (c)
biacyl triplet formed by the coupling of two acyl radicals.
The photon emissio...
Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
... of bovine serum albumin. Yields of p24 and RTB/
p24 recombinant proteins were estimated at 4.6 mg/l and
0.63 mg/l respectively.
Immunoblot analysis
Analysis by immunoblot of the recombinant purified ... 1/200, and levels of IgG anti-p24 were determined by ELISA. Analysis was performed in dupli-
cate and all sera samples were analyzed in triplicate and standard deviations...
Báo cáo y học: "Smoking and nicotine exposure delay development of collagen-induced arthritis in mice" pptx
... hours
of incubation, the supernatants were collected and frozen at -
70°C for future analysis.
Measurements of antibody and cytokine levels
Quantification of anti-collagen II antibodies in serum ... Parsons
ME: An investigation into the effect and mechanisms of action
of nicotine in inflammatory bowel disease. Inflamm Res 2000,
49:311-319.
14. Wang H, Liao H, Ochani M, J...
Báo cáo y học: " Recombination and base composition: the case of the highly self-fertilizing plant Arabidopsis thaliana" pdf
... GC content and codon
bias in A. thaliana, and to use models to examine the joint
effects of recombination and inbreeding on selection on
codon usage and BGC, in order to help us to interpret the
results ... possibly high rates of
inbreeding in budding yeast.
In C. elegans, the level of codon bias is intermediate between
that of A. thaliana and Drosophila [34], and...
Báo cáo y học: " Research and Policy, Stanford University School of" pdf
... saber.
(a)
(b...
Báo cáo y học: "Early and mid term mortality after coronary artery bypass grafting in women depends on the surgical protocol: retrospective analysis of 3441 on- and off-pump coronary artery bypass grafting procedures" doc
... retrospective analysis of 3441 on- and off-pump coronary
artery bypass grafting procedures. Journal of Cardiothoracic Surgery 2010
5:90.
Submit your next manuscript to BioMed Central
and take full advantage ... 1.7%.
Mortality in men was 2.1% after 30 days and 3.7% after one year c) gender specific mortality: 30 days mortality in
women was 1.7% using OPCAB and 5.2% using ECC (...
Báo cáo y học: " Joint and tendon subclinical involvement suggestive of gouty arthritis in asymptomatic hyperuricemia: an ultrasound controlled study" pps
... sign),
whereas inhomogeneous tendon and/ or entheseal thick-
ening and intratendinous hyperechoic bands defined the
presence of enthesopathy or tendinopathy. Erosion was
defined as a definite cortical interruption ... [24] and Sjögren’s syndrome [25]. This
prompted us to investigate its ability to identify the invol-
vement of the hyaline cartila ge, tendons and joints in
individ...
Báo cáo y học: "Cough and dyspnea during bronchoconstriction: comparison of different stimul" doc
... and coughing counts were
made during bradykinin, methacholine and exercise
induced bronchoconstriction. The study showed that
bradykinin induced more cough and dyspnea than meth-
acholine and exercise. ... could increase the
sensitivity of the test by increasing cough intensity.
In addition, during challenge tests, cough and dyspnea
were significantly more intense in subjects...
Báo cáo y học: "Entropy and bispectral index for assessment of sedation, analgesia and the effects of unpleasant stimuli in critically ill patients: an observational study" potx
... (BIS-Index, RE, SE, 60 sec mean values) and
physiological parameters were recorded continuously, and
were analyzed at 30 and 2 minutes before the event (time
points -30 and -2), and at 2, 5 and ... GE
Healthcare. Instrumentarium/Datex-Ohmeda was not involved
in any way in collection, analysis and interpretation of data, in
writing of the manuscript, or in the decisio...
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