Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx

... Access Production and purification of VP2 protein of porcine parvovirus expressed in an insect- baculovirus cell system Hongchao Zhou 1† , Guizhe Yao 2† , Shangjin Cui 2*† Abstract The porcine parvovirus ... costly than bacterial expression systems for producing viral proteins and may present difficulty in purifying expressed proteins from insect cell an...

Ngày tải lên: 11/08/2014, 21:21

... 0) and the enzyme solution. Reactions were started by adding H 2 O 2 and were quantified by monitoring the initial rate of increase in absorbance at 470 nm in the presence and absence of manganese. ... (a) singlet oxygen, (b) triplet carbonyls from dioxetane and a-hydroxyperoxyl radicals, and (c) biacyl triplet formed by the coupling of two acyl radicals. The photon emissio...

Ngày tải lên: 24/03/2014, 04:21

... of bovine serum albumin. Yields of p24 and RTB/ p24 recombinant proteins were estimated at 4.6 mg/l and 0.63 mg/l respectively. Immunoblot analysis Analysis by immunoblot of the recombinant purified ... 1/200, and levels of IgG anti-p24 were determined by ELISA. Analysis was performed in dupli- cate and all sera samples were analyzed in triplicate and standard deviations...

Ngày tải lên: 12/08/2014, 04:21

... hours of incubation, the supernatants were collected and frozen at - 70°C for future analysis. Measurements of antibody and cytokine levels Quantification of anti-collagen II antibodies in serum ... Parsons ME: An investigation into the effect and mechanisms of action of nicotine in inflammatory bowel disease. Inflamm Res 2000, 49:311-319. 14. Wang H, Liao H, Ochani M, J...

Ngày tải lên: 09/08/2014, 14:21

... GC content and codon bias in A. thaliana, and to use models to examine the joint effects of recombination and inbreeding on selection on codon usage and BGC, in order to help us to interpret the results ... possibly high rates of inbreeding in budding yeast. In C. elegans, the level of codon bias is intermediate between that of A. thaliana and Drosophila [34], and...

Ngày tải lên: 09/08/2014, 20:20

... saber.                                                                                                                                                                                                (a) (b...

Ngày tải lên: 09/08/2014, 20:21

... retrospective analysis of 3441 on- and off-pump coronary artery bypass grafting procedures. Journal of Cardiothoracic Surgery 2010 5:90. Submit your next manuscript to BioMed Central and take full advantage ... 1.7%. Mortality in men was 2.1% after 30 days and 3.7% after one year c) gender specific mortality: 30 days mortality in women was 1.7% using OPCAB and 5.2% using ECC (...

Ngày tải lên: 10/08/2014, 09:22

... sign), whereas inhomogeneous tendon and/ or entheseal thick- ening and intratendinous hyperechoic bands defined the presence of enthesopathy or tendinopathy. Erosion was defined as a definite cortical interruption ... [24] and Sjögren’s syndrome [25]. This prompted us to investigate its ability to identify the invol- vement of the hyaline cartila ge, tendons and joints in individ...

Ngày tải lên: 12/08/2014, 15:22

... and coughing counts were made during bradykinin, methacholine and exercise induced bronchoconstriction. The study showed that bradykinin induced more cough and dyspnea than meth- acholine and exercise. ... could increase the sensitivity of the test by increasing cough intensity. In addition, during challenge tests, cough and dyspnea were significantly more intense in subjects...

Ngày tải lên: 13/08/2014, 08:20

... (BIS-Index, RE, SE, 60 sec mean values) and physiological parameters were recorded continuously, and were analyzed at 30 and 2 minutes before the event (time points -30 and -2), and at 2, 5 and ... GE Healthcare. Instrumentarium/Datex-Ohmeda was not involved in any way in collection, analysis and interpretation of data, in writing of the manuscript, or in the decisio...

Ngày tải lên: 13/08/2014, 11:22