production and purification of k5

... PPV-VP2 FD and PPV-VP2 FD The fragment was about 1840 bp 3.2 Expression and purification of polyhistidine-tagged VP2 of PPV We have previously shown that formation of chimeric VLPs of PPV fusion ... cultures of porcine origin The method is both labor intensive and costly, with the additional hazard of requiring the handling of large quantities of infectious virus [21] Economic and safety ... produce VP2 VLPs of infectious bursal disease virus [19], and parvoviruses including PPV [14] and B19 [15] To further facilitate the use of PPV VP2 for diagnosis and vaccination, the goal of the current...

Ngày tải lên: 11/08/2014, 21:21

... a volume of 30 μl of PBS, as follows: PBS containing μg of CT adjuvant (Sigma), 15 μg of p24, 30 μg of RTB/p24, 15 μg of p24 mixed with μg of CT and, 30 μg of RTB/p24 mixed with μg of CT adjuvant ... weight mass of the predicted translation products from sequences RTB/p24 and p24 was 57 kDa and 34 kDa respectively E coli expression of RTB/p24 and p24 proteins Purification of RTB/p24 and p24 was ... chromatography purification E coli expression of RTB/p24 and p24 HIV proteins Six E coli strains were tested to obtain the best levels of expression and solubility of the RTB/p24 and p24 proteins...

Ngày tải lên: 12/08/2014, 04:21

... Harvesting/concentration and cold storage of rotifers 3.7 Production and use of resting eggs 3.8 Literature of interest 3.9 Worksheets Worksheet 3.1 Preparation of an indicator solution for determination of residual ... Standard enrichment for Great Salt Lake Artemia 4.4 Tank production and use of ongrown Artemia 4.4.1 Nutritional properties of ongrown Artemia 4.4.2 Tank production 4.4.2.1 Advantages of tank production ... Literature of interest CLADOCERANS, NEMATODES AND TROCHOPHORA LARVAE 6.1 Daphnia and Moina 6.1.1 Biology and life cycle of Daphnia 6.1.2 Nutritional value of Daphnia 6.1.3 Feeding and nutrition of Daphnia...

Ngày tải lên: 15/12/2013, 00:15

... These various aspects of the production, use and substitution of micro-algae in aquaculture will be treated within the limits of this chapter 2.2 Major classes and genera of cultured algal species ... reproducibility, which is a function of the sampling, diluting, and filling of the counting chamber, as well as the choice of the right type of counting chamber and range of cell concentration Counting ... the growth of diatoms which utilize this compound for production of an external shell Micronutrients consist of various trace metals and the vitamins thiamin (B1), cyanocobalamin (B12) and sometimes...

Ngày tải lên: 15/12/2013, 00:15

... 3.7 Production and use of resting eggs For the mass rearing of rotifers as larval food the amictic way of reproduction (see 3.3.) should be favored However, when the interest is in production of ... growth of the animal is assured by plasma increase and not by cell division The epidermis contains a densely packed layer of keratin-like proteins and is called the lorica The shape of the lorica and ... the profile of the spines and ornaments allow the determination of the different species and morphotypes (see 3.4.) The rotifers body is differentiated inTO three distinct parts consisting of the...

Ngày tải lên: 15/12/2013, 00:15

... outputs, not only in terms of survival, growth and success of metamorphosis of many species of fish and crustaceans, but also with regard to their quality, e.g reduced incidence of malformations, improved ... resulted in optimized and cost-effective applications of this live food in hatchery production 4.1.2 Biology and ecology of Artemia 4.1.2.1 Morphology and life cycle 4.1.2.2 Ecology and natural distribution ... screening of candidate strains and of eventual local populations, as well as the study of prevailing environmental conditions Uncontrolled introduction of Artemia may thus lead to a decrease of natural...

Ngày tải lên: 15/12/2013, 00:15

... content and hatchability Hatchability of cysts is largely determined by the conditions and techniques applied for harvesting, cleaning, drying and storing of the cyst material The impact of most of ... function of the tank size and the density of cysts incubated Excessive foaming can be reduced by disinfection of the cysts prior to hatching incubation and/ or by the addition of a few drops of a ... The use of decapsulated cysts in larval rearing presents some distinct advantages, both from a practical and nutritional point of view The daily production of nauplii is labour intensive and requires...

Ngày tải lên: 15/12/2013, 00:15

... fast harvesting of large volumes of Artemia nauplii and allows complete removal of debris from the hatching medium This technique results in a significant reduction of labour and production costs ... Ph and Lavens, P 1995 Review of some aspects of marine fish larviculture ICES mar Sci Symp., 201:138-142 Sorgeloos, P and Léger, Ph 1992 Improved larviculture outputs of marine fish, shrimp and ... harvest and start enrichment 18:00 add second enrichment · Day - 9:30 harvest 4.4 Tank production and use of ongrown Artemia 4.4.1 Nutritional properties of ongrown Artemia 4.4.2 Tank production...

Ngày tải lên: 15/12/2013, 00:15

... Enrichment of ongrown Artemia 4.4.2.8 Control of infections 4.4.2.9 Harvesting and processing techniques 4.4.2.10 Production figures and production costs 4.4.2.1 Advantages of tank production and tank ... behavior of Artemia by affecting the filtration rate, ingestion rate and/ or assimilation: including the quality and quantity of the food offered, the developmental stage of the larvae, and the ... leaching of body fluids (See also 4.5.7.1) 4.4.2.10 Production figures and production costs Figure 4.4.14 provides a summary of average production data expressed as Artemia survival and length,...

Ngày tải lên: 15/12/2013, 00:15

... are usually low and mainly fluctuate in function of food availability, temperature and salinity The size and/ or often complete absence of suitable infrastructure makes management of such lakes ... very unprecise and inaccurate Of course sample volume depends both on animal abundance and animal size As both cyst production and biomass production mainly depend on the number of large animals, ... usually depends on the availability of high saline water and is often limited to certain periods of the year Management of these ponds is similar to the management of the Artemia ponds in artisanal...

Ngày tải lên: 15/12/2013, 00:15

... Literature of interest 6.1 Daphnia and Moina 6.1.1 Biology and life cycle of Daphnia 6.1.2 Nutritional value of Daphnia 6.1.3 Feeding and nutrition of Daphnia 6.1.4 Mass culture of Daphnia 6.1.5 Production ... intensity, ) and biota (phytoplankton and copepod production, number of predators, bacterial turn-over, regeneration of nutrients from copepods and fish larvae) in such a way that the production of one ... nauplius and copepodite stages of copepods; · 300 and 500 µm for small water fleas and smaller species of cyclopoid copepods; · 700 µm for adult water fleas of the Daphnia genus, large species of cyclopoid...

Ngày tải lên: 15/12/2013, 00:15

... by using sieves 6.1.5 Production and use of resting eggs Resting eggs are interesting material for storage, shipment and starting of new Daphnia cultures The production of resting eggs can be ... concentrations of suspended material can interfere with the uptake of food particles Figure 6.1 Schematic drawing of the internal and external anatomy of Daphnia 6.1.4 Mass culture of Daphnia 6.1.4.1 ... amylases, lipases and even cellulase, that can serve as exo-enzymes in the gut of the fish larvae 6.1.3 Feeding and nutrition of Daphnia The filtering apparatus of Daphnia is constructed of specialized...

Ngày tải lên: 15/12/2013, 00:15

... Foundation of Raisiogroup (Raisio, Finland), the Finnish Funding Agency for Technology and Innovation (TEKES) and the Commission of the European Communities, specifically RTD program ‘Quality of Life and ... low production yields of the enzymes In this article, the cloning, production and characterization of a novel tyrosinase from the filamentous fungus T reesei is reported Results Isolation of a ... Da) The identity of this peptide was then confirmed by MALDI-TOF ⁄ TOF fragment ion analysis, where partial sequences of this peptide were obtained from the ladders of b-fragment and y-fragment...

Ngày tải lên: 19/02/2014, 06:20

... ratios of 33·5% and 16·4% in NAICS 51211 (motion picture and video production) and NAICS 51219 (post -production and other motion picture and video industries), respectively.9 This high level of concentration ... modern production system may be represented in terms of the size of its component units and the standardization (or variability) of their outputs This idea is codified in Fig 1, where scale and standardization ... tier Second of all, some independent production companies work partially in the one tier and partially in the other, and others move erratically in and out of the sphere of operation of the Hollywood...

Ngày tải lên: 07/03/2014, 15:20

... from H2O2 production during the autoxidation of dopamine following the addition of varying amounts of the diphenol into solutions of NaCl ⁄ Pi (pH 7.4) that lacked catalase (A and B) and those ... tetrahydrobiopterin; GSH, reduced glutathione compounds, and reducing agents, the activities of melanogenic enzymes and competitive oxidases, and the availability of enzyme cofactors The two-step reaction sequence ... given to understanding the role of peroxidase in the early stages of melanogenesis, despite reports of the involvement of peroxidase–H2O2 systems in later stages during the oxidation of indolequinone...

Ngày tải lên: 07/03/2014, 17:20

... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ether/acetonitrile/acetone 2:1:1 and 4:3:1 v/v/v) are 0.34 and 0.24, and 0.20 and 0.20, respectively, which ... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and...

Ngày tải lên: 07/03/2014, 21:20

... of the head and swelling of the mandibular and Bakhat Baidar Khan, Arshad Iqbal and Muhammad Riaz    University of Agriculture, Faisalabad.  Part – III Production and Management of Camels cervical ... University of Agriculture, Faisalabad.  Part – III Production and Management of Camels Q Give a tabulated comparison of some of the anatomical characteristics of the camel with those of bovines and ... Bakhat Baidar Khan, Arshad Iqbal and Muhammad Riaz    University of Agriculture, Faisalabad.  Part – III Production and Management of Camels can multiply and grow Upon stagnation of growth, autolysis of bacterial...

Ngày tải lên: 08/03/2014, 23:20

... sequence of the expression clone was confirmed by sequence analysis of both DNA strands Production and purification of ADH E coli BL21(DE3) harbouring pSJS1244 was transformed with pWUR78 and a single ... Machielsen and J van der Oost Metals and inhibitors The effect of several salts, metals and inhibitors on the initial activity of Pf-TDH was checked using butane2,3-diol as substrate in the standard ... reaction and acetoin in the reduction reaction The activity of Pf-TDH was significantly increased by the addition of mm CoCl2 (relative activity to that of the standard reaction 170%) and not by...

Ngày tải lên: 16/03/2014, 14:20

... proteins and characterized from structural and enzymatic points of view The immunoreactivity of these two proteins has also been studied Results Production, isolation, and spectroscopic and structural ... candidate for a major determinant of the immunoreactivity of these proteins Aspf1, another protein belonging to the ribotoxin family, is a major and one of the best-characterized allergens of ... characteristics of the NH2terminal b-hairpin of ribotoxins, we have studied its involvement in functional and immunoreactive properties of the protein On the basis of these ideas, the allergen Aspf1 and...

Ngày tải lên: 16/03/2014, 19:20