báo cáo khoa học: " Molecular and functional analyses of COPT/Ctrtype copper transporter-like gene family in rice" pdf

... presence of three distinct domains: the rigid CORE-domain and two smaller peripheral domains or mobile parts, the NMP-binding site and the LID-domain [4]. The NMP-binding site has many intermolecular contacts ... were obtained by simple rotation of the same model. The flexible LID domain appears at top of the three figures, NMP-binding site and ATP binding domain are displayed, NH 2...

Ngày tải lên: 21/02/2014, 00:20

... Access Molecular and functional analyses of COPT/Ctr- type copper transporter-like gene family in rice Meng Yuan, Xianghua Li, Jinghua Xiao and Shiping Wang * Abstract Background: The copper ... COPT1, COPT5, and COPT7 and slightly suppressed COPT4 in root and induced COPT5, COPT6, and COPT7 in shoot. Fe deficiency slightly induced COPT1 and suppressed CO...

Ngày tải lên: 11/08/2014, 11:22

... F.M. (1999) Identification of amino acids in the N-terminal domain of corticotropin-releasing factor receptor 1 that are important determinants of high-affinity ligand binding. J. Neuro- chem. 72, ... equal volume of DMEM media containing 10% fetal bovine serum was added and cells were incubated overnight. Next morning, the cells were washed by DMEM and incubated in DMEM media co...

Ngày tải lên: 07/03/2014, 15:20

... identified a protein containing a single ankyrin repeat that interacts with HIV-1 viral protein R (Vpr) and we designated this protein as Vpr-binding ankyrin repeat protein (VBARP). This interaction ... ankyrin repeat motif-containing proteins play an important role in protein–protein interactions. ANKHD1 proteins are known to possess multiple ankyrin repeat domains and a single KH domai...

Ngày tải lên: 07/03/2014, 21:20

... the first and last turns of helices E and F, and the C-terminus helices I and J. In addition, there is a lysine-rich N- and C-ter- minus, in accordance with the basic isoelectric point of CagS. ... transcription factor-jB and the induction of pro -in ammatory pro- duction of cytokines, mainly interleukin (IL)-8 [12]. The activation of a pro -in ammatory signalling cas...

Ngày tải lên: 06/03/2014, 00:21

... Expression of cardosin genes during flower development and in several organs of C. cardunculus (A) Control analysis of the specifici- ty of the PCR amplification of each cardosin gene. Phage DNA including ... FEBS Discussion Cloning by library screening of four full-length genes encoding cardosins A, B, C and D precursors, together with the cloning of a partial sequence of...

Ngày tải lên: 30/03/2014, 09:20

... activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remains unclear and it is uncertain ... and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PR...

Ngày tải lên: 15/02/2014, 01:20

... missing; in chain B, residues 167–172 are missing; in chain C, residues 1, 2 and 169–174are missing; in chain D, residues 168–174 are missing; in chain F, residues 1 and 168–175 are missing. All ... (NCS) restraints were applied to residues 5–160 and 193–230 with tight main-chain and medium side-chain restraints. Model building was carried out in o [29] and coot [30]. In...

Ngày tải lên: 18/02/2014, 16:20

... ending in the middle of the transmembrane domain 6 was amplified using the specific primers, TRHR10 and TRHR7, and a template corresponding to a mixture of the PCR products obtained in (A) and ... especi- ally xTRHR3 and xTRHR2. This is particularly interesting considering the functional importance of the third intracel- lular loop and the C-terminal tail in receptor cou...

Ngày tải lên: 21/02/2014, 03:20

... function in peroxisome biogenesis remains unknown. In this study we mapped the mutual binding sites and assayed the effects of deletion and point mutations in Pex1p and Pex6p for the interaction of ... demonstrate the inverse importance of D1 and D2 in Pex1p and Pex6p for the interaction of these two proteins. While a fragment of Pex6p consisting of N and D1 al...

Ngày tải lên: 07/03/2014, 16:20