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báo cáo khoa học: " Molecular and functional analyses of COPT/Ctrtype copper transporter-like gene family in rice" pdf

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... presence of three distinct domains: the rigidCORE-domain and two smaller peripheral domains ormobile parts, the NMP-binding site and the LID-domain[4]. The NMP-binding site has many intermolecularcontacts ... wereobtained by simple rotation of the same model. The flexible LIDdomain appears at top of the three figures, NMP-binding site and ATPbinding domain are displayed, NH2- and COOH-termini are alsoindicated.Ó ... Within the AMP bindingdomain, some amino acid residues are well conserved(L59, F87, R89) while two others, a leucine and a valine,are replaced by V35 and I60, respectively. A Mg2+binding...
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báo cáo khoa học:

báo cáo khoa học: " Molecular and functional analyses of COPT/Ctrtype copper transporter-like gene family in rice" pdf

... Access Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in riceMeng Yuan, Xianghua Li, Jinghua Xiao and Shiping Wang*AbstractBackground: The copper ... COPT1, COPT5, and COPT7 and slightlysuppressed COPT4 in root and induced COPT5, COPT6, and COPT7 in shoot. Fe deficiency slightly inducedCOPT1 and suppressed COPT2 and COPT5 in roo t and induced ... type of assay according tothe manufacturer’s instructions. In this system, COPTproteins fused with both the C-terminal half of the ubi-quitin protein (Cub) and the mutated N -terminal half of the...
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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

... F.M. (1999) Identification of amino acids in the N-terminaldomain of corticotropin-releasing factor receptor 1 that areimportant determinants of high-affinity ligand binding. J. Neuro-chem. 72, ... equalvolume of DMEM media containing 10% fetal bovineserum was added and cells were incubated overnight. Nextmorning, the cells were washed by DMEM and incubated in DMEM media containing 5% fetal bovine ... 2004 Molecular and functional characterization of novel CRFR1 isoformsfrom the skinAlexander Pisarchik and Andrzej SlominskiDepartment of Pathology and Laboratory Medicine, University of Tennessee...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

... identified a proteincontaining a single ankyrin repeat that interacts withHIV-1 viral protein R (Vpr) and we designated thisprotein as Vpr-binding ankyrin repeat protein(VBARP). This interaction ... ankyrin repeat motif-containing proteins play an important role in protein–protein interactions. ANKHD1 proteins are known to possessmultiple ankyrin repeat domains and a single KH domain with ... tyrosine kinase (RTK) sign-aling, independent of MAP kinase (MAPK) by eitherfunctioning downstream of MAPK or by defining anew pathway of RTK signaling [8]. RTKs play import-ant roles in cell...
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Báo cáo khoa học: Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island potx

Báo cáo khoa học: Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island potx

... the first and lastturns of helices E and F, and the C-terminus helices I and J. In addition, there is a lysine-rich N- and C-ter-minus, in accordance with the basic isoelectric point of CagS. ... transcriptionfactor-jB and the induction of pro -in ammatory pro-duction of cytokines, mainly interleukin (IL)-8 [12].The activation of a pro -in ammatory signallingcascade has been confirmed ... anti-parallel to thesame strand of a second monomer, allowing for theformation of the dimer. The surface of interactionbetween monomers also involves portion of chains D and E of the two monomers,...
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Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. docx

Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. docx

... Expression of cardosin genes during flower development and in several organs of C. cardunculus (A) Control analysis of the specifici-ty of the PCR amplification of each cardosin gene. Phage DNA including ... FEBSDiscussionCloning by library screening of four full-length genesencoding cardosins A, B, C and D precursors, togetherwith the cloning of a partial sequence of the cyprosin B gene and the isolation of ... stages of the plantlife cycle [16–21]. In this study, the genomic sequences of the cardo-sin A and B genes and of two new cardosin genes (thoseencoding cardosins C and D) were isolated and charac-terized....
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... activity of PRTFDC1towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remainsunclear and it is uncertain ... and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine.Table 2. Km and Vmaxvalues for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 ... However, a tight binding of N7 of the purine base and Asp137 was observed in HPRT in complex with ImmGP, indicating a role in transitionstate stabilization during catalysis [17].A series of mutations...
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Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

... missing; in chain B, residues 167–172 are missing; in chain C, residues1, 2 and 169–174are missing; in chain D, residues 168–174are missing; in chain F, residues 1 and 168–175 are missing.All ... (NCS)restraints were applied to residues 5–160 and 193–230 withtight main-chain and medium side-chain restraints. Modelbuilding was carried out in o [29] and coot [30]. In chain A, residues 1 and ... usually found in nucleotide binding enzymes is not present in UpUMPK[19]. UpUMPK contains instead a glycine-rich motifwithin amino acids 44–54 that is responsible for bind-ing of the phosphate...
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Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt

Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt

... ending in the middle of the transmembrane domain 6 wasamplified using the specific primers, TRHR10 and TRHR7, and atemplate corresponding to a mixture of the PCR products obtained in (A) and ... especi-ally xTRHR3 and xTRHR2. This is particularly interestingconsidering the functional importance of the third intracel-lular loop and the C-terminal tail in receptor coupling and regulation. ... of N-glycosylation sites in the gonadotropin-releasing hormonereceptor: role in receptor expression but not ligand binding. Mol.Cell. Endocrinol. 107, 241–245.47. Nussenzveig, D.R., Heinflink,...
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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

... function in peroxisome biogenesisremains unknown. In this study we mapped the mutual binding sites and assayed the effects of deletion and point mutations in Pex1p and Pex6p for the interaction of ... demonstrate the inverseimportance of D1 and D2 in Pex1p and Pex6p for theinteraction of these two proteins. While a fragment of Pex6p consisting of N and D1 alone exhibits signifi-cant binding properties ... do interact in vivo. We assigned their corresponding binding sites and elucidated theimportance of ATP-binding and -hydrolysis of Pex1p and Pex6p for theirinteraction. We show that the interaction...
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