báo cáo khoa học: " Functional mapping of reaction norms to multiple environmental signals through nonparametric covariance estimation" ppsx
báo cáo khoa học: " Functional mapping of reaction norms to multiple environmental signals through nonparametric covariance estimation" ppsx
... 11:23 http://www.biomedcentral.com/1471-2229/11/23 Page 9 of 13 METH O D O LOG Y AR T I C LE Open Access Functional mapping of reaction norms to multiple environmental signals through nonparametric covariance estimation John ... of different environmental factors. Results: We implement a more robust nonparametric covariance estimator to model these interact...
Ngày tải lên: 11/08/2014, 11:21
Báo cáo khoa học: Functional implications of pigments bound to a cyanobacterial cytochrome b6f complex potx
... rate of 0.7 mLÆmin )1 . (A) Acetone extract of purified cyt b 6 f of the PS1-less mutant. (B) Absorbance spectrum of echinenone. (C) Acetone extract of purified cyt b 6 f of the PS1-less ⁄ CrtO-less ... the absorption spectrum of chloro- phyll and the redox state of a b-type cytochrome. To determine the orientations of the various cofac- tors with respect to the long axis...
Ngày tải lên: 07/03/2014, 16:20
Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... that, according to prior studies of nonheme Fe 2+ centers [29–31], is assigned to the mixing of the 4p orbital with the 3d orbital of the metal cofactor. Two important pieces of information can ... number of the Fe 2+ center [29–31]. The value of (12 ± 1) · 10 )2 eV there- fore indicates that the Fe 2+ bound to Bxe_A2876 is coordinated by a total of five ligand atoms. An in...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... correct position to initiate the catalytic reaction. The transition state of this type of kinases is considered to be close to trigonal bipyramidal geo- metry of the phosphate to be transferred. ... mutagenesis of four active site residues of Dm-dNK. Residue E52 was mutated to D, H and Q, residue Y70 to W, residue Q81 to N and residue R105 to K and H. The kinetic pr...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... interaction of DEC1 with BMAL1 has been suggested a s one of the molecular mechanisms of the suppression [Honma, S., Kawamoto, T., Takagi, Y., Fujimoto, K., Sato, F., Noshiro, M., Kato, Y. & ... 10 ng) of FLAG-DEC1 expression vector were co- transfected with pDEC1-E-ABC-TK, together with expression vectors for CL OCK and BMAL1. The total amount of transfected DNA was adjustedtot...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in fibrinolysis and plasmin-induced cell detachment and apoptosis docx
... and to a hyperbolic decay equation that allows calculation of the concentration of mAb that produces 50% of the inhibitory effect (IC 50 ) [61]. Effect of anti-LBS mAbs on the activation of plasminogen ... microscope. The apoptotic index was calculated as the percentage of TUNEL-positive nuclei relative to total DAPI-stained nuclei. Effect of anti-LBS mAbs on fibrinolysis The...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx
... their ability to bind to the junction in vitro. The truncations did not prevent the localization of Ydc2 to mitochondria but the mutants showed no activity in vivo. Significantly, the loss of Ydc2 led to ... Subcellular localization of Ydc2 and truncated mutants to yeast mitochondria. Fluorescence micrographs of Ydc2–EGFP fusions in ydc2D S. pombe cells (green), MitoTracker s...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx
... the dimerization of EF-Ts in the crystal. In the crystal of E. coli EF-Tu–EF-Ts, a quaternary complex, formed by two molecules of each of the elongation factors, is observed. The coiled-coil motifs of each of ... is 78% of the point of growth inhibition of UY211 (D 420 ¼ 0.60). The point of growth inhibition of GRd.tsf when expressed as a percentage of that of UY211,...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... measured in the presence of acti- vators (pyruvate or PEP) and inhibitors (NADH or ATP) (Fig. 5A). The results indicate that PEP is able to reverse, in part, the inhibitory effects of both NADH and ATP ... (Fig. 5A). In the case of pyruvate, although partial reversal of NADH inhibition was observed, total reversal of ATP inhibition was found (Fig. 5A). The regulatory properties o...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
... with the binding of 15E11 to the intact repeat region (Fig. 6C,D). The residual binding of 15E11 to FnBRAD9,10 could be a consequence of the weak binding of repeats 4 and 5 to the antibody. 15E11 ... 3. SPR analysis of NTD binding to FnBRs of FnBPA and FnBPB fused to GST. Different concentrations of the NTD were compared for binding to GST–repeats immobilized on the...
Ngày tải lên: 06/03/2014, 22:21