báo cáo khoa học: " Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo" pdf

... purposes) In vivo conjugation of QD's to Akt-PH-EGFP via intein mediated protein splicingFigure 1 In vivo conjugation of QD's to Akt-PH-EGFP via intein mediated protein splicing. (a) ... domain with the N-terminus half of a split intein (I N ). The C-terminus half (I C ) of the intein was conjugated to QDs in vitro. I C -QD's and RNA encoding PH-I N wer...

Ngày tải lên: 11/08/2014, 00:22

... MultiSemCor Corpus. Journal of Natural Language Engineering, 11(3):247–261. Adam Bermingham and Alan F. Smeaton. 2009. A study of inter-annotator agreement for opinion re- trieval. In Proceedings of ACM SIGIR, ... Journal of Natural Language Engineering, 11(3):311–325. Hiroshi Kanayama. 2003. Paraphrasing rules for au- tomatic evaluation of translation into Japanese. In Proceedi...

Ngày tải lên: 24/03/2014, 03:20

... preferential binding of the avian influenza A viruses to α-2,3 linkages in type II pneumocytes of the lower respiratory tract of humans and a vigorous cytokine response, including increased interleukin-6, ... specificity of influenza A [6,7]. Avian influenza subtypes prefer to bind to sialic acid linked to galactose by α-2,3 linkages, which are found in avian intestinal an...

Ngày tải lên: 13/08/2014, 03:20

... policy to obtain CXRs after insertion of endotra- cheal tubes, intravenous lines and chest drains, but not after insertion of nasogastric tubes. In addition, CXRs were obtained in the case of worsening ... adjustment of an invasive device. Our findings are in accordance with those of this previous study, at least in part. Indeed, in our study most CXR-induced changes wer...

Ngày tải lên: 25/10/2012, 10:39

... obtained using the Cdc2 and Cdc25C promoters. In G 0 , proteins appear to bind to the CDE ⁄ CHR, as monitored by in vivo footprinting. Accord- ing to these early experiments all binding is lost in ... function MKLP1 ⁄ KIF23 mitotic kinesin-like protein 1 ⁄ kinesin family member 23 Kinesin-like protein, motor enzyme that moves antiparallel microtubules, localizes to the interzo...

Ngày tải lên: 16/02/2014, 09:20

... presence of pseudouridines in 70S but not in free 50S subunits can be explained by reduced rates of pseudouridine formation due to the transition of A1916 to G. The effects of the mutations in 23S ... 50 lL of water (10 min at room temperature). Determination of pseudouridines Pseudouridines were determined according to the method of Ofengand [34]. Fifteen micrograms...

Ngày tải lên: 18/02/2014, 16:20

... of successive deletions of terminal amino acid residues of all six hydrophobins providing this single match. A rapid means of identifying peaks corresponding to hydrophobins in MALDI-TOF mass spectra ... identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS Torsten Neuhof 1 , Ralf Dieckmann 1, *, Irina S. Druzhinina 2 , Christian P. K...

Ngày tải lên: 19/02/2014, 02:20

... prag- matics of word meaning. In Journal of Linguistics, pages 387–414. M. Pasca and S. Harabagiu. 2001. The informative role of WordNet in open-domain question answer- ing. In Proceedings of NAACL-01 ... give for the metonymic phrase. But they are not meaningful as such for automatic pro- cessing, since their senses still need to be disam- biguated in order to obtain the...

Ngày tải lên: 20/02/2014, 09:20

... RA (2001) The photo- cycle of a flavin-binding domain of the blue light photoreceptor phototropin. J Biol Chem 276, 36493– 36500. W. Eisenreich et al. 13 C NMR of phototropin domains FEBS Journal ... receptor phototropin comprises a protein kinase domain and two FMN-binding LOV domains (LOV1 and LOV2). Blue light irradiation of recombinant LOV domains is conducive to the addition of...

Ngày tải lên: 07/03/2014, 05:20

... of IbeA in solution using spectroscopic techniques to identify protein fold- ing intermediates. Results The initial step in the action of IbeA for E. coli K1 traversal of the BBB is binding to ... LB broth containing 100 lg Æ mL )1 of ampicillin at 37 °C. The overnight culture was inoculated to 1 L of fresh LB media containing 100 l g ÆmL )1 of ampicillin at 37 °C until...

Ngày tải lên: 07/03/2014, 05:20