báo cáo khoa học: "A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization" ppt

... number not for citation purposes)Journal of NanobiotechnologyOpen AccessShort CommunicationA novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronizationKensuke ... on-chip single- cell sorting method for cultivating particular cells chosenfrom clued mixture of cells [7], and have found the adap-tation process of epigenetic memorization in cells by stor-ing ... myocyte cells demonstrated thata single beating cell can influence the rate of a neighbour-ing cell in close contact and that a group of heart cells in aculture, beating synchronously with a rapid...

báo cáo khoa học: " A novel method for efficient and abundant production of Phytophthora brassicae zoospores on Brussels sprout leaf discs" pot

... found in the intercellularspace between mesophyll cells. The few haustoria thatwere observed were small and – like haustoria of P.infestans – digit-like in shape. In late stages of infection,hyphae ... Protein (GFP) taggedP. brassicae transformant. Microscopical examination of the infected tissues showed hyphal growth in leaves,stems and roots (Figure 1B, D, and 1F). In leaves extensiveintercellular ... are the infectiouspropagules. Since the current P. brassicae zoospore isolation methods are generally regarded asinefficient, we aimed at developing an alternative method for obtaining high...

Tài liệu Báo cáo khoa học: A novel type of highly negatively charged lipooligosaccharide from Pseudomonas stutzeri OX1 possessing two 4,6-O-(1-carboxy)-ethylidene residues in the outer core region ppt

... points using forward linear prediction extra-polation [28,29].MALDI-TOF analysisMALDI mass spectra were carried out in the negativepolarity in linear or in reﬂector mode on a Voyager STRinstrument ... were measured in the1H-detected mode via singlequantum coherence with proton decoupling in the13Cdomain, using data sets of 2048 · 512 points, and 64 scanswere acquired for each t1value. ... analysis of oligosaccharide monophosphatesobtained from the lipopolysaccharide of recombinant strains of Salmonella minnesota and Escherichia coli expressing the genus-speciﬁc epitope of Chlamydia...

Báo cáo khoa học: A novel mechanism of V-type zinc inhibition of glutamate dehydrogenase results from disruption of subunit interactions necessary for efﬁcient catalysis doc

... reduced cofactor in theenzyme–glutamate–reduced cofactor complexes. In theabsence of glutamate zinc appears to signiﬁcantlytighten NADPH binding. Interestingly in the presence of norvaline zinc ... 2.Effects of zinc on cofactor bindingThe effects of zinc on the binding of the reduced co-factor NADPH to the enzyme, at pH 8.0, in the pres-ence or absence of glutamate were examined usingﬂuorescence ... theability of zinc to inhibit the activity of this enzyme.The major zinc binding site is located in the GTPbinding site and probably inhibits the enzyme in a sim-ilar manner to GTP. Europium binds in...

Báo cáo khoa học: A novel inhibitor of indole-3-glycerol phosphate synthase with activity against multidrug-resistant Mycobacterium tuberculosis pptx

... (num-bering in red) of the 11 residues surrounding ATB107 within 5 A˚ in mIGPS (A) were also included in the 14 residues surrounding sub-strate within 5 A˚(B). (C) Binding regions of substrate ... the inhibitionmechanism of ATB107.To investigate the role of these residues in the inhib-itory effect of ATB107, we compared the binding sites of CdRP and of ATB107. The substrate-binding ... structure, screening wasperformed in a search for novel inhibitors, using the Maybridge databasecontaining the structures of 60 000 compounds. ATB107 was identiﬁed asa potential binding molecule;...

Báo cáo khoa học: A novel isoform of pantothenate synthetase in the Archaea potx

... 100 100Minus pantoate 2Minus ATP 46 50Minus ATP, minus pantoate 3Minus ATP, minus Pi45Minus ATP, minus ADP 3Eqn (1)Complete system 20Minus pantoate 1Minus ATP NDMinus ATP, minus pantoate ... differentprotein folds: the hexokinase, ribokinase, and galacto-kinase families of sugar kinases. Protein Sci2, 31–40.18 So¨ding J (2005) Protein homology detection by HMM–HMM comparison. Bioinformatics ... speciesproduce pantoate by combining an archaeal KPHMTisoform with bacterial-type KPR or by combining anarchaeal KPR isoform with bacterial-type KPHMT. In other words, the distribution of bacterial-typeKPHMT...

Báo cáo khoa học: A novel mechanism of TGFb-induced actin reorganization mediated by Smad proteins and Rho GTPases docx

... inhibitor of transcription actinomycin D,and changes in the actin cytoskeleton were monitoredby ﬂuorescence using rhodamine phalloidin staining. Asshown in Fig. 1A, TGFb caused potent actin ... organization of the actin cyto-skeleton, resulting in cell ﬂattening and cell shapechange. Cells appeared elongated or spindle-shaped,with a parallel arrangement of actin bundles (Fig. 5J).Addition of ... contain any of these putative Smad binding ele-ments. We are in the process of characterizing thesesites further and studying their role and participation in the transcriptional upregulation of...

Báo cáo khoa học: A novel factor XI missense mutation (Val371Ile) in the activation loop is responsible for a case of mild type II factor XI deﬁciency doc

... (1993)The linkage between binding of the C-terminal domain of hirudin and amidase activity in human alpha-throm-bin. Biochem J 289, 475–480.45 Baglia FA & Walsh PN (1996) A binding site for thrombin ... thrombin in absence of dextransulfate. Activation of both proteins by humana-thrombin followed pseudo-ﬁrst-order kinetics, asshown in Fig. 3. This was in agreement with a stochas-tic model of ... degree of structural change;this is especially true in the case of the introduction of an isoleucine, a b-branched amino acid that is not ﬂex-ible. In the active conformation, Val371 forms contactswith...

Báo cáo khoa học: "A Computational Analysis of Complex Noun Phrase in Messages" docx

... mechanisms for maximizing the amount of information that can he expressed within a limited time and space. These mechanisms include increased frequency of complex noun sequences and also increased ... Sager. Analysis and Pro- cussing of Compact Text. Proc. COLING 82, 201-206, North Holland. [Marsh 1083] Marsh, E. Utilizing Domain-Specific Infor- mation for Processing Compact Text. Proc. Conf. ... Grishman in pro- ceasing the initial corpus of Navy messages, and the efforts of the researchers at New York University in pro- cussing the medical record corpus. 508 the sublanguage of Navy...

Báo cáo khoa học: "A systematic understanding of probabilistic semantic extraction in large corpus" pptx

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