báo cáo khoa học: "Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications" pps

... of 16 (page number not for citation purposes) Ex vivo expansion and phenotype analyses of DC-activated immune effector cellsFigure 6 Ex vivo expansion and phenotype analyses of DC-activated immune ... CD137-selected immune effector cells after ex vivo expansionFigure 4 Effector function analyses of IFN-γ- or CD137-selected immune effector cells af...

Ngày tải lên: 10/08/2014, 22:20

... concentration was measured using Bradford reagent [27]. A similar protocol was used for the expression and purification of HPRT, with the exception that sonication was used instead of high pressure homogenization, ... HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PRPP, determined...

Ngày tải lên: 15/02/2014, 01:20

... D and E + F), (b) a few hydrogen bonds, and a hydrophobic interaction between A + C, B + E and D + F, and (c) electrostatic forces in the central channel of the hexamer between B, C and F, and ... Glu204 (Fig. 3A,C), and a hydrophobic interaction between Thr131 and Phe133 (Fig. 3D). The central channel of the hexamer is made up of two layers of electrostatic forces on...

Ngày tải lên: 18/02/2014, 16:20

... logarithmic and stationary phases (Fig. 3C). Overexpression and purification of AK2 The cultures of bacterial cells (XL1-Blue) transformed with pQE30-AK were induced with IPTG for overexpression of AK2 ... pattern for both substrates, with K i values of 190 n M and 160 n M for ATP and AMP, respectively. Ap5A also inhibited growth of L. donovani promastigotes in...

Ngày tải lên: 21/02/2014, 00:20

... Xenopus and mouse TRHR1) [41]. Tyr106 and Asn110 have been reported to form hydrogen bonds with the pyroGlu residue of TRH and Arg306 with the ProNH 2 Fig. 5. Ca 2+ imaging experiments on HEK-293 cells ... protein of 401 amino acids (45.2 kDa) for xTRHR2 and 397 amino acids (45.0 kDa) for xTRHR1. Alignment of the deduced amino acid sequences with that of xTRHR3 (Fi...

Ngày tải lên: 21/02/2014, 03:20

... long a-helices and a final b-strand, F, which protrudes from the core of the monomer and runs anti-parallel to the same strand of a second monomer, allowing for the formation of the dimer. The surface of ... structure of only three of these unique components (CagZ, CagS and CagD) is now available, along with Caga ATPase. The lack of a clear picture of the biological f...

Ngày tải lên: 06/03/2014, 00:21

... where exons 6 and 13 are missing (AF180301); CRH-R1e with deletion of exons 3 and 4 (AF369651); CRFR1f with deletion of exon 12 (AF369652); CRFR1g with deletion of exon 11, 27 basepairs of exon 10 and ... the only isoform coupled directly to adenylate cyclase with the exception of an artificial isoform (CRFR1h2) with the insertion of 37 amino acids between the ligan...

Ngày tải lên: 07/03/2014, 15:20

... coimmunoprecipitation analysis with HsPex1p (from fibroblasts of individuals with wild-type PEX1 and of patients carrying PEX1 alleles mutated in D1) and HsPex6p, which show a lowered Pex6p binding to Pex1p mutated ... and I. Birschmann et al. Domain function of Pex1p and Pex6p FEBS Journal 272 (2005) 47–58 ª 2004 FEBS 57 Structural and functional analysis of the intera...

Ngày tải lên: 07/03/2014, 16:20

... Exon–intron analysis of VBARP isoforms. Exons and introns present in VBARP-L and VBARP-S are represented as boxes with numbers. The length in base pairs of each exon is marked on top of the boxes representing ... NT2 cells were transfected with different concentrations of VBARP siRNA or control siRNA and assayed for functional effects. First, to confirm that VBARP A B F...

Ngày tải lên: 07/03/2014, 21:20

... determined with K d ¼ c 2 m =c d ð3Þ Culture and transfection of HeLa cells HeLa cells were grown on 22 · 22 mm cover slips placed in 6-well plates with 2 mL of MEM (with Earle’s salts and glutamine) with ... protein composed mainly of a helices (Figs 1 and 2). The core of Iba2 is a pair of EF-hand motifs, denoted as EF-hands 1 and 2, each consisting of two a he...

Ngày tải lên: 16/03/2014, 06:20