báo cáo khoa học: " Antitumor activity of mixed heat shock protein/ peptide vaccine and cyclophosphamide plus interleukin-12 in mice sarcoma" pot

... immunomodulators. Vaccine 2007, 25s:b72-b88. doi:10.1186/1756-9966-30-24 Cite this article as: Guo et al.: Antitumor activity of mixed heat shock protein /peptide vaccine and cyclophosphamide plus interleukin-12 ... Clinical Cancer Research 2011, 30:24 http://www.jeccr.com/content/30/1/24 Page 2 of 9 RESEARCH Open Access Antitumor activity of mixed heat s...

Ngày tải lên: 10/08/2014, 10:21

... conformational transition upon binding to BchH may bring the integrin I domain of BchD into contact with the integrin-binding motif of BchH, simultaneously triggering porphyrin metallation. This would ... one type of molecular chaperones and their function is to control the fate of proteins or DNA. This is done by facilitating protein folding and unfolding, assembly and disassem...

Ngày tải lên: 19/02/2014, 12:20

... expressing this protein, and m ass s pectrometry confirmed that the isolated protein was indeed maiz e EF-Tu. The purified protein was capable of binding GDP (indicative of protein activity) and was ... than 20% of the original CS activity remained after 75 min of recovery (Fig. 6A). In contrast, when 2 l M CS was heated at 43 °C in the presence of 4 l M pre-EF-Tu, 46% of...

Ngày tải lên: 23/03/2014, 13:20

... comparing the position of the absorbance peak (280 nm) of the stand- ard marker with that of aspartyl aminopeptidase. Effect of inhibitors on aspartyl aminopeptidase activity Solutions of various inhibitors ... printing using MALDI-TOF MS. In- gel digestion and peptide mass fingerprinting The protein band on each SDS ⁄ polyacrylamide gel was cut into small pieces which were in...

Ngày tải lên: 07/03/2014, 12:20

... J (2001) Heat shock protein 27 shows a distinctive wide- spread spatial and temporal pattern of induction in CNS glial and neuronal cells compared to heat shock protein 70 and caspase 3 following kainite ... proinflammatory prostaglandins and cytokines [34–36]. Ionized calcium- binding adaptor molecule 1 (iba-1) is a calcium-bind- ing protein that is specifically expressed...

Ngày tải lên: 18/02/2014, 17:20

... examined cal- pain 7–IST1 interactions by in vitro and in vivo binding experiments, using purified recombinant proteins and cultured mammalian cells expressing epitope-tagged proteins. We also investigated ... whether MIM1 and MIM2 bind to only one MIT domain or bind to each of the two MIT domains of calpain 7, and it is not known why interaction between calpain 7 and the MIM...

Ngày tải lên: 18/02/2014, 04:20

... features of the catalytic site (i.e. a catalytic triad of Ser-His-Glu, an oxyanion hole, a p-cation-binding site, and an acyl-binding pocket) are the same in AChE and BuChE (Fig. 1). The acyl-binding pocket, ... J.C. (2003) Crystal structure of recombinant human butyrylcholinesterase: new insights into the catalytic mechanisms of cholinesterases. Proceedings of the XIth Cholin...

Ngày tải lên: 20/02/2014, 23:20

... environment and are essentially an indication of substrate specificity. Full understanding of sHsp substrate interactions will require not only considera- tion of substrate binding sites and binding interactions, ... deter- mined by the N-terminal arm. The N-terminus of PsHsp18.1.C-I was also implicated in substrate interactions in bis-ANS binding experiments [15]. As it is pro...

Ngày tải lên: 07/03/2014, 15:20

... from the N-terminus domain of histone H2A: buforin I (39 amino acids), parasin I (21 amino acids), and hipposin (51 amino acids ) from the Asian toad stomach and catfish and halibut skin mucus, respect- ively, ... antimicrobial peptides are also derived from larger proteins by proteolysis. For example, bovine lactoferricin is derived from lactoferrin, an iron bin ding protein, by pepsi...

Ngày tải lên: 07/03/2014, 16:20

... supernatant. Fig. 6. Autophosphorylation of recombinant Ndk and mutant proteins. (A) Ndk and mutant proteins (1 lg) were incubated in the presence of 10 lCi of [c- 32 P]ATP in 20 lL of reaction volume. The reaction ... [14]. Production of polyclonal anti-Ndk Ig Purified Ndk protein (50 lg) was solubilized in 500 lLof Freund’s incomplete adjuvant and injected into Swiss al...

Ngày tải lên: 08/03/2014, 08:20