Molecular characterization of the PhoPQ-PmrDPmrAB mediated pathway regulating polymyxin B resistance in Klebsiella pneumoniae CG43 ppt

... manner. Analysis of < /b> EMSA indicates a direct binding of < /b> the < /b> recombinant PhoP to pmrD The < /b> binding of < /b> PhoP or Pm rA to P pmrH has been deter- mined recently [31]. In order to determine whether PhoP binds directly ... in F igure 5A, binding of < /b> the < /b> recombinant His- PhoP protein to P pmrD was evident by the < /b> formation of < /b> a protein/D...

Ngày tải lên: 10/08/2014, 05:21

... almost continuous density for the < /b> backbone of < /b> CBH IB. The < /b> final model, 1Q9H, included 430 of < /b> the < /b> 437 amino acid residues of < /b> CBH IB. The < /b> final two amino acids and the < /b> loop region (from amino acids 193–197) ... perhaps indicative of < /b> its flexibility. It is worth noting that the < /b> structures of < /b> T. re esei GH7 CBHs were solved...

Ngày tải lên: 23/03/2014, 13:20

... by the < /b> Scientific Services Department of < /b> the < /b> Weizmann Institute by a single injection of < /b> 150 lg of < /b> the < /b> purified protein, followed by two booster shots of < /b> the < /b> same amount of < /b> protein at 2-week intervals. Serum ... Alexander Girshovich who initiated and was actively involved in the < /b> beginning of < /b> this work. We wish to th...

Ngày tải lên: 22/02/2014, 07:20

... polymerase activity on the < /b> temperature was determined by assaying the < /b> enzyme in the < /b> standard reaction mixture at the < /b> indicated temperatures. (D) Thermal stability of < /b> Rep245 was tested by pre-incubating the < /b> enzyme ... not governed by the < /b> sequence of < /b> the < /b> substrates used for these assays was further supported by the < /b> finding t...

Ngày tải lên: 18/02/2014, 18:20

... characteristics of < /b> the < /b> lac1 protein suggest the < /b> absence of < /b> a type 1 copper site, this is not borne out by analysis of < /b> the < /b> deduced amino acid sequence of < /b> the < /b> enzyme. Thus, the < /b> 10 histidines and one cysteine ... gene expression but also positively affected the < /b> activity and stability of < /b> the < /b> enzyme [40]. The < /b...

Ngày tải lên: 07/03/2014, 15:20

... of < /b> OSBP proteins in sterol and sphingo- lipid metabolisms. The < /b> canonical OSBP is believed to play a role in regulating < /b> sterol biosynthesis, Keywords OSBP; OSH; Osh6p; oxysterol-binding protein; ... N-terminal half containing the < /b> conserved ORD domain mediates lipid binding. A weak binding to PtdIns(3)P by all proteins was also observed, which might be nonspecific...

Ngày tải lên: 07/03/2014, 21:20

... hydrolysis of < /b> bovine Hb. Bovine Hb (1 lg) was incubated with rHlSCP1 in 25 m M citrate ⁄ 50 mM phosphate buffer (pH 4–7) or 50 m M phosphate buffer (pH 8). Co, reaction buffer containing bovine Hb without ... initially cleaves in the < /b> conserved hinge region of < /b> the < /b> Hb alpha chains [49,50]. Falcipain, a member of < /b> the < /b> cysteine protease family, degrade...

Ngày tải lên: 16/03/2014, 10:20

... here. The < /b> binding of < /b> TTR to RBP has been shown to stabilize the < /b> binding of < /b> retinol to RBP. Studies of < /b> the < /b> interactions of < /b> chimaera of < /b> RBP and epididymal retinoic acid binding pro- tein (ERABP) ... RBP in the < /b> core of < /b> the < /b> b- barrel. When bound, retinol is almost totally encapsulated by the < /b> protein, the < /...

Ngày tải lên: 16/03/2014, 23:20

... protein in rat brain In order to clarify the < /b> localization of < /b> the < /b> 105-kDa protein in the < /b> brain, the < /b> rat brain was dissected into six sections and Table 1. Properties of < /b> the < /b> 105-kDa protein, APG-1, ... sequence of < /b> the < /b> 105-kDa protein To investigate the < /b> biochemical properties of < /b> the < /b> 105-kDa protein, the <...

Ngày tải lên: 17/03/2014, 10:20