Trypsin-induced proteome alteration during cell subculture in mammalian cells potx

... reproduction in any medium, provided the original work is properly cited. Research Trypsin-induced proteome alteration during cell subculture in mammalian cells Hsiang-Ling Huang 1 , Hsiang-Wei Hsing 1 , ... results. Detection of trypsin-induced differential protein expression in cervical cancer cells It is interesting and important to know whether trypsine- induced...

Ngày tải lên: 10/08/2014, 05:21

... of DNA in a single cell cycle. Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7]. Targets of the kinase in the regulation of DNA replication include ... named mitotic (M) cells. Residual cells were collected and named G 2 cells. These cells and logarithmically grow- ing cells were separated into Triton-soluble (S) and Triton...

Ngày tải lên: 16/03/2014, 13:20

... knockout cells) and CasWt cells (generated by transfecting CasKo cells with wild-type Cas). Cas expression in CasWt cells was similar to that in Balb3T3 cells, and Cas was absent in CasKo cells (Fig. ... migrating cells. Scratch-induced elongation of protrusions and reorientation of the Golgi were more prominent in cells that expressed Cas than in CasKo cells or cell...

Ngày tải lên: 23/03/2014, 03:20

... killing following binding (recently reviewed in [1]). Whilst a variety of cell types including plant cells, fungal cells and even bacteria [2] have been described as hosts, mammalian cells are the most ... of interest for rapidly selecting from a lib- rary reliable intrabodies in mammalian cells [5]. In addition, the fact that only b-gal-expressing CHO cells were rescued...

Ngày tải lên: 19/02/2014, 18:20

... chromatin conden- sation. For assessment of proportion of apoptotic cells, the number of cells on cover slips and the number of detached cells were determined. Immunoblot analysis Cells (2 · 10 5 cells ... For heat shock treatment, cells were treated in a water bath set at 45 °C for 15–120 min. Cell viability assay Cells (1 · 10 4 cells per well) in 96-well plates were in...

Ngày tải lên: 16/03/2014, 06:20

... were transfected into 3T3 cells using superfect lipid reagent. The transfected cells were grown in the medium containing 0, 0.008, 0.04 and 0.2 l M concentrations of RG-102240. The cells were harvested ... were transfected into 3T3 cells using superfect lipid reagent. The transfected cells were grown in the medium containing 0, 0.2, 1 and 5 l M concentrations of RG-102240. The...

Ngày tải lên: 16/03/2014, 12:20

... of SA on stress response in mammalian cells using a simple screening system, and revealed that SA is a potent Hsp inducer in mammalian cells, thereby protecting cells against deleterious stress. Experimental ... obtained and maintained in DMEM containing 0.2 mgÆmL )1 G418. Measurement of Luc activity pGL105C3H cells (2 · 10 5 cells per 35 mm dish) were washed with NaCl/P i...

Ngày tải lên: 17/03/2014, 10:20

... machinery of the mammalian cell [1]. The members of the above g ene families work together in a variety of cellular p rocesses, including p rotein folding during which the hsp70s bind unfolded, ... chaperoning function [15] or the binding to membranes [16]. Dj2 was found to cooperate with hsc70 in assisting the folding of denatured proteins [10,17] and i n participating in the pro...

Ngày tải lên: 17/03/2014, 17:20

... [21,22]. In platelets and neuronal cells, Glut3 is also present in intracellular ves- icles [3,23]. These results imply distinctive roles for Glut1 and Glut3 in mammalian cells, although they remain ... C-terminal domains and the extracellular loops are distinctive [1,13] (Fig. 1B). Glut3 is expressed at the cell surface of var- ious types of cell, including neuronal cells [13]...

Ngày tải lên: 23/03/2014, 09:20