Antibody Phage Display Methods and Protocols - part 8 docx

... Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 28 Generation of Bispecifi c and Tandem Diabodies Sergey ... to Modify Immunoglobulins 313 303 From: Methods in Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and...

Ngày tải lên: 10/08/2014, 00:21

... Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 1 Overview of Antibody Phage- Display Technology and Its Applications Hennie ... with complementary methods to increase the scope and range of applications of this technology. 2. Antibody Phage Display 2.1. The Phage- Displa...

Ngày tải lên: 10/08/2014, 00:21

... 2,2′-di-p-nitrophenyl-5,5′,-diphenyl-3, 3 -[ 3,3′-dimethoxy-4,4′-diphenylene] ditetrazolium chloride and 0. 38 mM 5-bromo-4-chloro-3-indoxyl phosphate mono-(p-toluidinium) salt in 0.1 M Tris- HCl, ... (5) and (6) unbound free and labeled hapten removed by washing. 187 From: Methods in Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by...

Ngày tải lên: 10/08/2014, 00:21

... Methods in Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 18 Selecting Antibodies to Cell-Surface ... Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 16 Proximity-Gui...

Ngày tải lên: 10/08/2014, 00:21

... Biol. Chem. 2 68, 5302–53 08. 85 . He, M. and Taussig, M. J. (1997) Antibody- ribosome-mRNA (ARM) complexes as effi cient selection particles for in vitro display and evolution of antibody combining ... agarose gels and from solution. 8. Appropriate phagemid vector (e.g., MC03) (Fig. 1). 9. Low-melting-temperature agarose. 10. Long-wave, hand-held UV lamp. 11. UV-transpare...

Ngày tải lên: 10/08/2014, 00:21

... TC-3′ HuV λ 6-Back-APA 5′-ACC GCC TCC ACC AGT GCA CTT AAT TTT ATG CTG ACT CAG CCC CA-3′ HuV λ 7 / 8- Back-APA 5′-ACC GCC TCC ACC AGT GCA CAG RCT GTG GTG ACY CAG GAG CC-3′ HuV λ 9-Back-APA 5′-ACC ... CC-3′ HuV λ 3B-Back-APA 5′-ACC GCC TCC ACC AGT GCA CTT TCT TCT GAG CTG ACT CAG GAC CC-3′ HuV λ 4-Back-APA 5′-ACC GCC TCC ACC AGT GCA CAC GTT ATA CTG ACT CAA CCG CC-3′ HuV λ 5-Back-APA 5′-ACC ....

Ngày tải lên: 10/08/2014, 00:21

... ACC 133 From: Methods in Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 9 Panning of Antibody Phage- Display ... solution. Panning Ab Phage- Display Libraries 141 147 From: Methods in Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols...

Ngày tải lên: 10/08/2014, 00:21

... nine autoclaved 1 . 8- mL microcentrifuge tubes as follows: TG 1-1 0 –1 , TG 1- 10 –2 , TG 1-1 0 –3 , TG 1-1 0 –4 , CJ23 6-1 0 –4 , CJ23 6-1 0 –5 , CJ23 6-1 0 –6 , CJ236–10 –7 , and CJ23 6-1 0 8 . Into each, pipet ... M Tris-HCl, pH 7.4, to neutralize the pH and mix gently. Isolation of Single-Chain Antibodies 239 269 From: Methods in Molecular Biology, vol. 1 78: An...

Ngày tải lên: 10/08/2014, 00:21

... E., and Robinson, R. R. (1 988 ) Secretion of functional antibody and Fab fragment from yeast-cells. Proc. Natl Acad. Sci. USA 85 , 86 78 86 82. 2. Shusta, E. V., Raines, R. T., Pluckthun, A., and ... molecules. 380 de Graaf et al. 367 From: Methods in Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitke...

Ngày tải lên: 10/08/2014, 00:21

... Differential Display 7 H-T110 H-T110 H-TIIA H-WA H-TIIA H-TIIC H-TllC H-AP3 H-APB H-API H-AP3 H-AP3 H-APl H-AP3 Fig. 2. Differential display using one-base anchored oligo-dT primers (7). ... 1262,4 3-5 1. 7 Ito, T and Sakaki, Y (1996) Fluorescent differential display method for htgh- speed scanning of tissue- or cell-specific transcripts Methods 4401 Genet 8, 22 9-2 4...

Ngày tải lên: 11/04/2014, 09:39