Antibody Phage Display Methods and Protocols - part 4 doc

... solution. Panning Ab Phage- Display Libraries 141 147 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana ... ACC 133 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., T...

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... Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 1 Overview of Antibody Phage- Display Technology and Its Applications Hennie ... with complementary methods to increase the scope and range of applications of this technology. 2. Antibody Phage Display 2.1. The Phage- Display...

Ngày tải lên: 10/08/2014, 00:21

... e.g., combine 0 .4 mM 2,2′-di-p-nitrophenyl-5,5′,-diphenyl-3, 3 -[ 3,3′-dimethoxy -4 , 4′-diphenylene] ditetrazolium chloride and 0.38 mM 5-bromo -4 - chloro-3-indoxyl phosphate mono-(p-toluidinium) salt ... (5) and (6) unbound free and labeled hapten removed by washing. 187 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edite...

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... (Pharmacia Biotech cat. no. 2 7-9 41 1-0 1); fl uorescein-isothiocyanate-conjugated anti-sheep Ab; anti-M13 Ab (Pharmacia Biotech cat. no. 2 7-9 41 0-0 1). 15. Tetramethyl-benzidine dihydrochloride (TMB) ... 10 8 1980.0 40 4.0× 1/512 24/ 24 (100) 4 2.87 × 10 11 3.08 × 10 8 1100.0 111.1× 1/10 24 a “0” represents the initial, unpanned Fab /phage library; b total number of...

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... 53.6 0 .4 <0.1 0.22 0.20 0. 34 2.36 15 52.5 <0.1 52.5 54. 6 0 .4 <0.1 0.23 0.20 0 .42 2.90 16 53.0 <0.1 53.0 55.5 0 .4 <0.1 0.23 0.20 0.57 4. 00 18 54. 0 <0.1 54. 0 57.5 0 .4 <0.1 ... tumor-targeting specifi city (Fig. 1). Smaller Ab-derived molecules include enzymatically produced 50-kDa Fabs and engineered 25-kDa single-chain Fvs (scFvs) consisting of heavy...

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... Construction Protocols 53 39 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 2 Standard ... agarose gels and from solution. 8. Appropriate phagemid vector (e.g., MC03) (Fig. 1). 9. Low-melting-temperature agarose. 10. Long-wave, hand-held UV lamp. 11. UV-tr...

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... surface. J. Immunol. 1 64, 47 30 47 41. 84 O’Brien and Aitken 87 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana ... TCA GG-3′ V H Forward HuJ H 1/2-For 5′-TGA GGA GAC GGT GAC CAG GGT GCC-3′ HuJ H 3-For 5′-TGA AGA GAC GGT GAC CAT TGT CCC-3′ HuJ H 4/ 5-For 5′-TGA GGA GAC GGT GAC CA...

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... nine autoclaved 1.8-mL microcentrifuge tubes as follows: TG 1-1 0 –1 , TG 1- 10 –2 , TG 1-1 0 –3 , TG 1-1 0 4 , CJ23 6-1 0 4 , CJ23 6-1 0 –5 , CJ23 6-1 0 –6 , CJ236–10 –7 , and CJ23 6-1 0 –8 . Into each, ... M Tris-HCl, pH 7 .4, to neutralize the pH and mix gently. Isolation of Single-Chain Antibodies 239 269 From: Methods in Molecular Biology, vol. 178: Antibody...

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... established phage- display technology or other molecules. 380 de Graaf et al. 367 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and ... LaVecchio, J., and Winkler, A. (1999) Human anti-HIV-1 tat scFv intrabodies for gene therapy of advanced HIV-1-infections and AIDS. J. Immunol. Methods 231, 223–23...

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... Differential Display 7 H-T110 H-T110 H-TIIA H-WA H-TIIA H-TIIC H-TllC H-AP3 H-APB H-API H-AP3 H-AP3 H-APl H-AP3 Fig. 2. Differential display using one-base anchored oligo-dT primers (7). ... Acta 1262 ,4 3-5 1. 7 Ito, T and Sakaki, Y (1996) Fluorescent differential display method for htgh- speed scanning of tissue- or cell-specific transcripts Methods 44 01 Genet 8, 22...

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