Antibody Phage Display Methods and Protocols - part 3 ppsx

... TCA GG -3 V H Forward HuJ H 1/2-For 5′-TGA GGA GAC GGT GAC CAG GGT GCC -3 HuJ H 3- For 5′-TGA AGA GAC GGT GAC CAT TGT CCC -3 HuJ H 4/5-For 5′-TGA GGA GAC GGT GAC CAG GGT TCC -3 HuJ H 6-For 5′-TGA ... CC -3 HuV λ 3B-Back-APA 5′-ACC GCC TCC ACC AGT GCA CTT TCT TCT GAG CTG ACT CAG GAC CC -3 HuV λ 4-Back-APA 5′-ACC GCC TCC ACC AGT GCA CAC GTT ATA CTG ACT CAA CCG CC -3 HuV λ 5-B...

Ngày tải lên: 10/08/2014, 00:21

... Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 1 Overview of Antibody Phage- Display Technology and Its Applications Hennie ... High-Level Periplasmic Expression and Purification of scFvs Sergey M. Kipriyanov 33 3 30 Periplasmic Expression and Purification of Recombinant Fabs Ro...

Ngày tải lên: 10/08/2014, 00:21

... CCA -3 HuJ H For3XhoI 5′-ACC GCC TCC ACC ACT CGA GAC GGT GAC CAT TGT CCC (TC)(TC)(GT) GCC CCA -3 HuJ H For 4-5 XhoI 5′-ACC GCC TCC ACC ACT CGA GAC GGT GAC CAG GGT TCC (TC)(TC)(TG) GCC CCA -3 HuJ H For6XhoI 5′-ACC ... display library-derived single-chain Fv antibody frag- ments. J. Immunol. Methods 239 , 1 53 166. 34 Hoogenboom Table 1 Murine LC Primer Sequences Gene family...

Ngày tải lên: 10/08/2014, 00:21

... brackets. Panning Ab Phage- Display Libraries 135 MCO phage- display vector system (2), which is derived from pComb3 (3) , and was specifi cally designed for the production, selection, and screening of Fab phage. ... ACC 133 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana P...

Ngày tải lên: 10/08/2014, 00:21

... 2,2′-di-p-nitrophenyl-5,5′,-diphenyl -3 , 3 -[ 3, 3′-dimethoxy-4,4′-diphenylene] ditetrazolium chloride and 0 .38 mM 5-bromo-4-chloro -3 - indoxyl phosphate mono-(p-toluidinium) salt in 0.1 M Tris- HCl, ... chaperone co-expression improves solubility and phage display of single chain antibody fragments. Protein Exp. Purif. 15, 33 6 34 3. Anti-Hapten Specifi c Ab Fragments 171...

Ngày tải lên: 10/08/2014, 00:21

... Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., Totowa, NJ 16 Proximity-Guided (ProxiMol) Antibody ... (Pharmacia Biotech cat. no. 2 7-9 41 1-0 1); fl uorescein-isothiocyanate-conjugated anti-sheep Ab; anti-M 13 Ab (Pharmacia Biotech cat. no. 2 7-9 41 0-0 1). 15. Tetra...

Ngày tải lên: 10/08/2014, 00:21

... autoclaved 1.8-mL microcentrifuge tubes as follows: TG 1-1 0 –1 , TG 1- 10 –2 , TG 1-1 0 3 , TG 1-1 0 –4 , CJ 23 6-1 0 –4 , CJ 23 6-1 0 –5 , CJ 23 6-1 0 –6 , CJ 236 –10 –7 , and CJ 23 6-1 0 –8 . Into each, pipet 30 0 µL ... M Tris-HCl, pH 7.4, to neutralize the pH and mix gently. Isolation of Single-Chain Antibodies 239 269 From: Methods in Molecular Biology, vo...

Ngày tải lên: 10/08/2014, 00:21

... Shuffl ing to Modify Immunoglobulins 31 3 30 3 From: Methods in Molecular Biology, vol. 178: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken © Humana Press Inc., ... (see Subheading 3. 1., steps 1–5, then Subheading 3. 2.) and reinfected into mutD5-FIT for further rounds of mutation (see Subheadings 3. 1. and 3. 2.), rescue (see Su...

Ngày tải lên: 10/08/2014, 00:21

... 22 Electroporation, methods, 52, 66, 94, 2 93, 30 9, 31 4, 35 2, 36 3, 36 5 preparation of cells, 51, 99, 2 93, 30 9, 35 2, 35 4, 36 3 ELISA, antigen capture, 115, 195, 198 8. pcDNA3 vector (Invitrogen, Leek, The Netherlands). ... 31 9 CD28, 31 9 CD36, 15 CD40, 121 CD55, 17 cDNA, storage, 55 synthesis, 47, 65, 75, 83, 90, 97, 117, 126, 211 c-erbB-2, 39 Chromatography, immobiliz...

Ngày tải lên: 10/08/2014, 00:21

... Differential Display 7 H-T110 H-T110 H-TIIA H-WA H-TIIA H-TIIC H-TllC H-AP3 H-APB H-API H-AP3 H-AP3 H-APl H-AP3 Fig. 2. Differential display using one-base anchored oligo-dT primers (7). ... performed m 30 cycles of 94°C for 30 s, 40°C for 2 mm, and 72°C for 30 s with a final extension of 5 min Run 32 Donohue, Hsu, and Winkles FGF- 1 M 0 ’ 2h 12h ’ - 281/271 -...

Ngày tải lên: 11/04/2014, 09:39