Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted proteins in parasites potx

... addressed the question of whether secreted proteins of parasitic nematodes show accel- erated evolution, and our results indicate that this is the case. The predominance of predicted secreted proteins ... of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted pro-teins in parasites& lt;p>...

Ngày tải lên: 09/08/2014, 20:20

... 9% unigenes) and Populus trichocarpa (212, 9% unigenes). The availability of the whole genome and predicted proteins of these species and limited sequence information of legumes in the databas ... i n 40%-60% of the total chickpea production [4]. Terminal drought, which occurs at the pod filling and seed-developing stage of the crop and incr eases in se...

Ngày tải lên: 11/08/2014, 11:22

... simultaneously interacts with the main phosphate of the substrate [32]. The closer positioning of the N-terminus to the main protein body is essential for the activity and, together with the 87–104 ... 23 diminished the midpoint of the thermal denaturation curve (T m )by7°Candthefree energy of unfolding (DG U )by11kJÆmol )1 . These values are in very close agreeme...

Ngày tải lên: 19/02/2014, 12:20

... between b-strand B6 in the substrate-binding domain and strand E2 in the hinge domain, two residues appear to act, at least partly, as structural determinants for the closed conformation of the substrate ... unfolding of the L537W ⁄ E542K and L537W ⁄ E542R mutants in one single heating cycle. The spectra of the native proteins L537W ⁄ E542K (solid line) and L...

Ngày tải lên: 07/03/2014, 03:20

... obtain an insight into the mechanism of thermosta- bility of this protein, we compared sequences of thermo- philic Bt-Lon with those of mesophilic Bs-Lon. The G+C content of the protein-coding ... Effects of temperature on the DNA-binding activity of Bt-Lon. 25 lL of the solution con- taining 4 lg Bt-Lon and 500 ng plasmid DNA was incubated for 20 min at the indic...

Ngày tải lên: 16/03/2014, 16:20

... It consists of the intrinsic subunits CP47, C P43, D2 and D1, and the extrinsic proteins of the oxyge n-evolving complex the 33 k Da, cyt c 550 and 12 k Da. The presence of the cyt c 550 in the PSII ... independent on the presence of other extrinsic proteins [44], whereas effective binding of red algal cyt c 550 to the red algal PSII requires the pres...

Ngày tải lên: 16/03/2014, 18:20

... 1/2 signal in the g ¼ 2region Fig. 1. P-cluster EPR signals of the MoFe protein compared to the EPR signals detected with the oxidized FeFe protein. TheMoFeprotein sample contained 2 1 mg protein ... the MoFe proteins of other bacteria. These include: demonstration o f the classical Fig. 6. EPR signals o f the FeFe protein and the MoFe protein under turnover condit...

Ngày tải lên: 18/03/2014, 01:20

... neovascu- larization of hES-ECs and hES-MCs could result in the increase in CBF and the marked promotion of vascular density in the ischemic striatum after the transplantation of hES-ECs+MCs. In the hES-EC+MC-injected ... used the term 'eEC' for these ECs in the early differentiation stage. We sorted and expanded these eECs in vitro. These eECs...

Ngày tải lên: 18/06/2014, 15:20

... reached the main sequence. The term pre-main sequence has been introduced since the first calculations of evolutionary tracks in the HR-diagram in the mid-1950s. 2.2 The Quest to Understand the Formation ... raising the issue of the stratification of the interstellar medium (ISM) in the gravitational potential of our Galaxy. Per- haps one of the bigges...

Ngày tải lên: 03/07/2014, 21:09

... arginine, respectively. Among the 135 streptococci investigated 94 and 4 of the strains fermented inulin and raffinose, respectively. Additionally, 130 of the investigated strains, and the S. uberis ... was produced by the majority of the S. uberis strains isolated from clinical cases of bovine mastitis. Comparable to these findings most of the S. uberis...

Ngày tải lên: 07/08/2014, 17:22