Báo cáo khoa hoc:"Mapping the Naked Neck (NA) and Polydactyly (PO) mutants of the chicken with microsatellite molecular markers" docx
... genetic map of the chicken, and open the way to the identification of the molecular nature of two develop- mental mutations of the chicken that are known to occur in many breeds of chickens. chicken ... mol´eculaire Mapping naked neck and polydactyly in chicken 79 Table I. Distribution of phenotypes and corresponding genotypes in the progeny of a...
Ngày tải lên: 09/08/2014, 18:21
... minimal. Morphological evidence of the antiapoptotic effect of HSS As shown in Fig. 2A,B, the treatment of cells with 50 lm CCCP induced profound changes in the nuclear morphology of hepatoma cells, with chromatin ... opening of the nonselective, highly conductive PTP in the mitochon- drial IM [39]. The exact molecular composition of the PTP remains unclear. Wh...
Ngày tải lên: 16/02/2014, 09:20
... 6-branch, and believed to be from a contaminant (see below), having the same molecular mass with one Fuc in each of the 3- and 6-branches. Comparison of the product-ion spectra of the singly and doubly ... Le a determinant. As the C 3a ion is at m/z 690, this excludes the possibility of the other Fuc being at the Gal next to the subterminal GlcNAc. The po...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: "Mapping between Compositional Semantic Representations and Lexical Semantic Resources: Towards Accurate Deep Semantic Parsing" docx
... behaviour of the verbs, which are not encoded in the SEM-I labelling and 191 also some contradictory annotations in the mapping between PropBank and VerbNet. Furthermore, the use of the WordNet ... for all Sentences and Parsings which include the current verb do 3: Initialize vertices of the network with SEM-I labels and fea- tures. 4: Initialize optionally verti...
Ngày tải lên: 08/03/2014, 01:20
Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx
... in exploring the regulatory behavior of this enzyme. To avoid misinterpretation of the results, the composition and stoichiometry of the bound cofactor(s) and the stability of the dimeric structure of ... The recovery of mono- meric epimerase from the spin column was 95% in terms of activity and 60% in terms of mass calculated on the basis of the di...
Ngày tải lên: 16/03/2014, 00:20
Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot
... Thus, the lipoate residues of the complex mediate the regulation of its first component by the last product of the overall reaction, NADH. Reduction of the lipoate residues of the complexes in the ... these domains. Rather, the advantage appears to depend on the ability of the lipoyl group to protrude from the inner core of the complex, indicative of...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: TioS T-TE – a prototypical thioesterase responsible for cyclodimerization of the quinoline- and quinoxaline-type class of chromodepsipeptides potx
... Encouraged by the results obtained, and to investigate the mechanism of macrocyclization, the steric demand of the C-terminal amino acid was further increased by the incorporation of l-Met4 (TL4) ... suggested that the size of the resulting ring and the preorganization of the substrate determine whether a ligation or a cyclization step is carried out [39]. Unfo...
Ngày tải lên: 23/03/2014, 06:20
Báo cáo khoa học: Succinate dehydrogenase flavoprotein subunit expression in Saccharomyces cerevisiae – involvement of the mitochondrial FAD transporter, Flx1p ppt
... obtain the new strains reported in Table 1. Genomic HA-tagging of SDH1 Three consecutive copies of the HA epitope were fused to the 3¢-end of the SDH1 ORF in the genome of both the WT and EBY167-G418 S strains, ... h of growth, each band represented about 30% of the total Sdh1- HA proteins. In the presence of FCCP (Fig. 4A, lane 2), a 60% reduction of the t...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: NrpRII mediates contacts between NrpRI and general transcription factors in the archaeon ¨ Methanosarcina mazei Go1 pot
... 0.25 and 0.5 lg of purified (His) 6 – NrpRII (lower panel) and 0.125, 0.25 and 0.5 lg of purified MBP–NrpRI (upper panel); elution in the absence of 2-oxoglutarate (lane 1), and in the presence of ... further verifying formation of a complex between NrpRI and NrpRII. The presence of 10 mm 2-oxoglutarate significantly decreased the binding of MBP–NrpRI to the ope...
Ngày tải lên: 29/03/2014, 21:20
Báo cáo khoa học: A pH-dependent conformational change in EspA, a component of the Escherichia coli O157:H7 type III secretion system potx
... 110 and 182, and no tryptophans. Therefore, the shape of the near-UV CD spectra of EspA suggests the presence of some ter- tiary contacts around at least one of the tyrosines both at pH 7.0 and ... cells [16]. Although there is information available concerning the roles and structural properties of EspA filaments, to date, the conformation and the thermodynami...
Ngày tải lên: 30/03/2014, 16:20