... protein concentrations keeping the initial concentration of CBC constant. The same final amplitude of fluorescent response was reached after 30 s of incubation, there- fore the reactions obeyed ... response for IF was, however, three-fold higher (Table 1). Binding of CBC to IF fragments IF 20 or IF 30 The binding reactions were conducted at constant CBC and variable concentrations of...
Ngày tải lên: 19/02/2014, 05:20
... experimental conditions occurs in 1 month; therefore, in the 1 month time-period between transfection and elimination, the parasite cul- tures are variable mixtures of transformed and untrans- formed ... linear conditions for protein concentra- tion and reaction time. ADC activities were calculated by measuring the radioactive CO 2 released during the reac- tions [24]. Protein concentra...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: "Types of Common-Sense Knowledge Needed for Recognizing Textual Entailment" ppt
... organiza- tion, they belong to that organization. 2. Preconditions : For a given action or event A at time t, a precondition p is a predicate that must hold true of the world before time t, in order for ... the κ of 0.640 for Mutual Exclusivity on its own. One merge that improves κ is a combination of Prominent Relation- ship with Argument Types (combined κ of 0 .250 , as comp...
Ngày tải lên: 20/02/2014, 05:20
Báo cáo khoa học: Improvement of ecdysone receptor gene switch for applications in plants: Locusta migratoria retinoid X receptor (LmRXR) mutagenesis and optimization of translation start site pptx
... Martinez A, Sparks C, Drayton P, Thompson J, Green- land A & Jepson I (1999a) Creation of ecdysone recep- tor chimeras in plants for controlled regulation of gene expression. Mol Gen Genet 261, ... Department of Entomology, University of Kentucky, Lexington, KY, USA 2 Plant and Soil Sciences Department, University of Kentucky, Lexington, KY, USA Introduction The conditional re...
Ngày tải lên: 15/03/2014, 23:20
Báo cáo khoa học: Construction of a novel detection system for protein–protein interactions using yeast G-protein signaling pdf
... agar. Construction of plasmids for yeast chromosome substitution Plasmids used for deletion of STE18 gene by substitution of the kanMX4 gene (G418 resistance gene) on the yeast chromosome were constructed ... ratio in the detection of protein–protein inter- actions. Detection of interactions in the absence of A BC Fig. 2. Schematic outline of gene construction. (A) Structu...
Ngày tải lên: 16/03/2014, 01:20
Báo cáo khoa học: Optimization of D-amino acid oxidase for low substrate concentrations – towards a cancer enzyme therapy pdf
... DAAO in the presence of high concen- trations of ammonia and pyruvate, conditions that induce formation of E-Fl red $P (see Scheme 1b): in this case, the time course of reoxidation is clearly biphasic. A ... photoreduction method [18]. In the present case, near-complete formation of the anionic semiquinone (‡ 95% on the basis of flavin content) was found for wt-DAAO, Q144R-DAAO...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
... gene, using a combination of ligation and recursive overlap extension of complementary synthetic oligonucleotides as described above. Oligonucleotides used for the construction of the s-CYPA are i ... transamination. In this situation, the main effect of transamination is a reduction of the degre e of 15 N-labelling in the amino a cid of interest. In thecaseof[ 15 N]Gly, much...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Activation of hepatocyte growth factor activator zymogen (pro-HGFA) by human kallikrein 1-related peptidases docx
... incubated for the same period without any cells is shown as ‘cell free’. hc, heavy chain of mature active form of HGF ⁄ SF. (B) Effects of engineered expression of KLK5 on cellular migration of SUIT-2. ... instructions. KLK3 and KLK4 were proforms, and required proteo- lytic activation using thermolysin followed by addition of phosphoramidon to inhibit excess thermolysin activ...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: "Adaptation of Statistical Machine Translation Model for Cross-Lingual Information Retrieval in a Service Context" ppt
... Cross-language information retrieval us- ing meta-language index construction and structural queries. In Proceedings of the 10th cross-language evaluation forum conference on Multilingual in- formation access ... of machine translation resources for english- japanese cross-language information retrieval. In In Proceedings of MT Summit VII Workshop on Ma- chine Translation for Cr...
Ngày tải lên: 24/03/2014, 03:20