Báo cáo khoa học: "Eag and HERG potassium channels as novel therapeutic targets in cancer" docx

... secretion [58]. Further investigation of HERG channels in cancer invasion and metastasis revealed that, in additio n to the high expression of herg gene and HERG protein in col- orectal cancers, ... variations in HERG currents in the mitotic cycle as shown in neuroblastoma cells [49]. The Eag and HERG channels have been shown to be inhibited in tissues of v...

Ngày tải lên: 09/08/2014, 03:24

... p53 inactivation occurred prior to invasion in breast carcinogenesis, with mutations being uniformly identified in ductal carcinoma in situ associated with p53 -mutated invasive carcinomas. Immunohistochemical ... markers in canine mammary carcinoma and female breast cancer [20,34]. Positive staining of p53 protein was detected in two benign mammary tumors accompanied by increase...

Ngày tải lên: 07/08/2014, 17:22

... accessing hydantoinases involved screening and iso- lating naturally occurring enzymes possessing hydan- toin-hydrolyzing activity from microbes, and using them to produce optically pure amino acids ... substrate binding pocket in the active site was inves- tigated based on the homology model to obtain more information on substrate binding in HYD Js . The orien- tation of the substrat...

Ngày tải lên: 18/02/2014, 08:20

... decrease in O 2 concentration in both the gas and liquid phases and the rapid increase in CO 2 concentration in both phases following the shift in benzoic acid concentration reflect a rapid increase ... process increases later, and is observed as an increase in OUR and CER as well as an increase in acetate (ethanol is converted back to acetate and produces $ 2 NAD...

Ngày tải lên: 07/03/2014, 04:20

... obvious in uence of stimulation. By contrast, on RML infection, an increase was observed in PrP Sc in LPS-stimulated cells ( 1.4-fold) after 24 h incubation without brain homogenate, and a slight increase ... and, after 48 h, also in CpG- ODN-treated cells (lane 11) was increased ( 1.8- and 1.3-fold, respectively) relative to the baseline signal directly after infection (lanes 2...

Ngày tải lên: 07/03/2014, 05:20

... carbohydrate-binding sites are indicated. Each B-chain domain is composed of three homologous subdomains a, b, c and linking k subdomain which joins the B-chain to the A-chain or the first B-chain domain ... Binding of the recombinant MLp A-chains with TA7 was detected in all samples. Interaction of the native A-chains with TA7 and MNA4 in comparison with recombinant A-chains showed th...

Ngày tải lên: 07/03/2014, 15:20

... collected and counted using trypan blue staining and ⁄ or cell viability assay kit (Promega). Caspase (3 ⁄ 7) activity was measured according the manu- facturer’s instructions using the Caspase-Glo3 ... protein containing a single ankyrin repeat that interacts with HIV-1 viral protein R (Vpr) and we designated this protein as Vpr-binding ankyrin repeat protein (VBARP). This interactio...

Ngày tải lên: 07/03/2014, 21:20

... a-heli- cal LID domain, situated in the C-terminal moiety, and the NMP-binding (NMP bind ) domain move in an induced-fit mechanism, closing upon binding of the phosphate donor and acceptor nucleotides, ... comparing the nucleobase recognition by eukaryotic UMP⁄ CMP kinases and bacterial CMP kinases. Thus, despite opposing hydrogen-bonding properties at positions 3 and 4 of the pyri...

Ngày tải lên: 16/03/2014, 10:20

... CPPs interesting for use as vectors for delivery of hydrophilic biomolecules and drugs into the cytoplasmic and nuclear compartments of the cell, both in vivo and in vitro [1–3]. The 60 amino ... differ- ence in chemical shift observed for Phe49 in penetratin and in penetratin(W48F,W56F) is most likely due to changes in ring currents when changing amino acid 48 from a tr...

Ngày tải lên: 23/03/2014, 18:20

... Enzyme activity was measured at increasing concentrations of Ca 2+ , using PC as substrate, at pH 9.5 and at 37 °C in the presence of 1 m M NaTDC. The star indicates the phospholipase activity measured in the ... min, and at room temperature for 5–10 min, and the absorbance was read at 620 nm. Bacterial strains, plasmids, and media E. coli strain DH5a was used as cloning host...

Ngày tải lên: 30/03/2014, 01:20