Báo cáo khoa học: " Vulnerability of young oak seedlings (Quercus robur L) to embolism: responses to drought and to an inoculation with Ophiostoma querci (Georgevitch) Nannf" potx

... Original article Vulnerability of young oak seedlings (Quercus robur L) to embolism: responses to drought and to an inoculation with Ophiostoma querci (Georgevitch) NannfG SimoninH ... wilt of susceptible and resistant tomato isolines.Spore Trans Phytopathol 61, 627-630Morelet M (1992) Ophiostoma querci sur chêne enFrance. Ann Soc Sci Nat Archéol Toulon, ... dépérissementINTRODUCTION Oak stands in Western and Central Europeare frequently reported to present severedieback symptoms. In France, pedunculate oak (Quercus robur L) is often declining,while...

Báo cáo khoa học: "Performance of young jack pine trees originating from two different branch angle traits under different intensities of competition" ppt

... and the means of the 0.75, 1.0 and 1.5m spacings, and between the means of the 0.75 and 1.0m spacings and the means of the 1.5 and 2.0 m spac-ings. The general trend was an increase in RGR with increase ... findings of Logan [31], Stewart and Hoddinott [60] and Noland etal.[44] for jack pine seedlings. Logan [31] observed thatgrowth took place under 13% of full sunlight, and Stewart and Hoddinott ... Kalra and Maynard [21].2.3. Growth analysesMorphological measures of crown development and measures of performance or efficiency as described byBrand [5], Hunt [18, 19] and Margolis and Brand...

Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf

... residue 29 (B), the side-chains of residues 29, T34 and E131 are shown. The hydrogen bonds betweenN29 and Oc and T34 and Oc, and between N29 and Nd and E131 and Oe2, in the wild-type protein ... residue 39 (C), the side-chains of Y24, residue 39, F41 and Q149 are shown. The hydrogen bondsbetween D39 and Od1 and Q149 and Ne, and between D39 and Od2 and Q149 and Ne, in the wild-type protein ... entire structure (A), N and C represent the N- and C-termini of the protein, and a and b represent the a helix and b strand,respectively. The side-chains of the mutated and parent amino acid residues...

Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf

... levels, and sLeaexpression in cancer cell lines and recombinant clonesWe also measured the levels of b3Gal-T5 transcript and b1,3Gal-T activity in different cancer cell lines and clones, and compared ... been shownthat sLex and poly N-acetyllactosamines are associated with increased malignancy of lung and colorectal cancers [7,8], and occur in core 2 and extended core 1 O-glycans invarious cells ... oligosaccharidesformed in BxPC3 cells (j in lower part, and A, B, and C of upper part)andT5ASclone(h in lower part, and A, B, and C of upper part)metabolically radiolabelled with [3H]Gal were characterized. Upperpart:...

Báo cáo khoa học: Mutants of Saccharomyces cerevisiae deﬁcient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling docx

... centrifugation and the supernatantwas extracted and tested for free fatty acids. Forstrains carrying a combined deletion of FAA1 and FAA2 or of FAA1 and FAA3, we did not observe anyfatty acid ... in an instantaneous rever-sion of the direction of net transport strongly argues for an active mechanism to achieve fatty acid export.It is important to note that the additional deletion of ... efﬁcient and result in aslower net transfer across the membrane. On the otherhand, the proposed model is able to balance the resultsobtained in the present study with the model of vecto-rial...

Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx

... both, GST-N and GST-C34, an hCaM band is only seen in the presence of Ca2+(lanes 5, 6 and 9, 10). In the absence of Ca2+(lanes 3, 4 and 7, 8) and for the GST control (lanes 1 and 2) there ... C- and N-terminal binding sites of hEAG1channels highlighted the importance of Ca2+-bindingability of EF-hand motifs 3 and 4 in CaM for its inter-action with the channel.ResultsRelevance ... volt-age-dependent activation of the channels according to Eqn 4. (D,E)Current recordings of hEAG1 (D) and hEAG1DN channels (E) inresponse to test depolarizations to +50 mV (D), and to )20 and )80 mV (E)...

Báo cáo khoa học: Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE pot

... (lanes 1 and 2), of HypED (lanes 3 and 4), HypE[D83N](lanes 5 and 6) and HypE[D83N A76V] ( lanes 7 and 8) were incubated with 0.5 lMHypF, 100 lM[14C]carbamoylphosphate and 100 lMATP(lanes1,3, 5and7 )or100lMADP-CH2-P ... standard; lanes 2 and 3: wild -type HypE p rotein; lanes 4 an d 5:HypE[D83N]; lanes 6 and 7: HypED. In lanes 2, 4 and 6 proteins(12 lM) were preincubated with 50 mMdithiothreitol fo r 1h ... carbamoylation reaction (lanes 3 and 4). The radioactive label in the D83N (lanes 5 and 6) and D83N A76V variants (lanes 7 and 8) is present in thethiocarboxamide form, irrespective of whether ATP orADP-CH2-P...

Báo cáo khoa học: Maturation of Pichia pastoris-derived recombinant pro-Der p 1 induced by deglycosylation and by the natural cysteine protease Der p 1 from house dust mite doc

... (bufferexchange to pH 4.0) and rpro-Der p 1 [18] [buffer exchange to pH 4.0, addition of cysteine, and heating to 60 °C (with/ without SDS)] did not result in maturation of the recom-binant pro-allergen ... Fridolin van der Lecq and others for t heir quick and excellent work on the protein s equences (Sequentie centrum, Utrecht,the Netherlands), and Dr Maurits de Planque for his explanations,time, and ... competitive RIA and sandwich ELISA with Der p 1-speci®c antibody reagents.CD spectra of r pro-Der p 1 and nDer p 1 revealed signi®-cant structural di erences. Deglycosylation of rpro-Der p 1 with endoglycosidase...

Báo cáo khoa học: Paralog of the formylglycine-generating enzyme – retention in the endoplasmic reticulum by canonical and noncanonical signals pptx

... (PGEL in human), split into the variable posi-tions 1 and 2, and the conserved EL ⁄ DL inpositions 3 and 4. Species with canonical retentionsignals are colored in blue, and noncanonical signalsin ... Tyr162 and Asn165 inKDELR1) located in three different transmembranehelices, which are highly conserved and found in allthree human KDEL receptor isoforms [29]. These and other data led to a ... medium, harvestedafter 0, 3 and 6 h of chase. Equal aliquots of precipitates from cells and medium were analyzed by SDS ⁄ PAGE and phosphorimaging.Bands were quantiﬁed; intracellularly retained...

Báo cáo khoa học: Characterization of a membrane-bound angiotensin-converting enzyme isoform in crayﬁsh testis and evidence for its release into the seminal ﬂuid ppt

... of protein of the [3H]hippurate obtained after incubation with and without10 lM captopril. Soluble (SOL) and insoluble (INSOL) fractions weretested in animals in active spermatogenesis and ... Description of stages. J BiophysBiochem Cytol 10, 301–333.17 Anderson WA & Ellis RA (1967) Cytodifferentiation of the crayﬁsh spermatozoon: acrosome formation, trans-formation of mitochondria and ... atrophied and are barely visible. At thecellular level (Fig. 4A), the testis is composed of acinithat open into collector canals and ﬁnally into a vasdeferens. The acini contain mesodermal cells and...

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