... of wild-type occludin (Occ WT ) and variant occludin in apoptosis and invasion, as determined by assay, we revealed that exon 9 played a major role in the induc- tion of mitochondria-mediated apoptosis and ... (sense) and 5Â-GAAAAAACGCGATCCTACTT-3Â (antisense). Primers for unmethylated DNA were: 5Â-GAAGTAGGTGGAGT ATTGAAT-3Â (sense) and 5Â-CAAAAAAACACAATCCT...
Ngày tải lên: 18/02/2014, 18:20
... application of SH2 domain binding peptides, which results in blocking of the binding of the SH2 domain to the substrate and thereby preventing interaction of the substrate with the kinase domain. For ... between Src family kinases (SFKs), stabilized by binding of the phosphorylated C-terminus to the SH2 domain and ⁄ or binding of the SH2 kinase...
Ngày tải lên: 06/03/2014, 01:20
Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationship between protein stabilization by osmolytes and its con- sequent ... protein stability and activity are not largely coupled in the presence of osmolytes. However, protein stability and activity have...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot
... 6E). From these results it can be concluded that tRNAs were bound in classical states with the tRNA Met f bound in the P-sites of the 30S and 50S subunits and the tRNA Phe bound in the A-site of the ... Production of this alarmone results in a down-regulation of stable RNA synthesis (rRNA and tRNA) and up-regulation of the synthesis of mRNAs encoding e...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae pot
... Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus in uenzae Martin Ma ˚ nsson 1 , Derek W. Hood 2 , E. Richard Moxon 2 and Elke ... Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK Structural analysis of the lipopolysaccharide (LPS) from nontypeable Haemophilus...
Ngày tải lên: 08/03/2014, 02:21
Báo cáo khóa học: Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 ppt
... Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1 ,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 Satoshi ... the assembly of the elementary unit of fibroin in ER It was demonstrated in this study that the elementary unit...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae An experimental and theoretical approach pot
... concom- itant appearance of inosine (Fig. 1A) . Incubation times longer than 30 min in the presence of H 2 O 2 did not greatly change the ratio SATP + ADP + AMP/Ino. Similar changes in ATP and inosine ... for 30 min, an important decrease in the ATP, and a less extensive decrease in the GTP, CTP, UTP and ADP-ribose levels was estimated. Concomitantly...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf
... A. niger (Eur. J. Biochem. 269) 607 Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme Eric Record 1 , Peter J. Punt 2 , ... clear band of around 70 kDa was observed corresponding to the wild-type laccase from P. cinnabarinus. Immunodetection of the laccase was performed usi...
Ngày tải lên: 17/03/2014, 11:20
Báo cáo khoa học: Paralog of the formylglycine-generating enzyme – retention in the endoplasmic reticulum by canonical and noncanonical signals pptx
... compilation ª 2008 FEBS Paralog of the formylglycine-generating enzyme – retention in the endoplasmic reticulum by canonical and noncanonical signals Santosh Lakshmi Gande 1 , Malaiyalam Mariappan 1 , ... pFGE by western blotting, using an antiserum against pFGE. The total expression level and the per- centage of intracellular pFGE are given. ER r...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Role of the plasma membrane leaflets in drug uptake and multidrug resistance ppt
... December 2009) doi:10.1111/j.1742-4658.2009.07555.x The present study aimed to investigate the role played by the leaflets of the plasma membrane in the uptake of drugs into cells and in their extrusion by P-glycoprotein and multidrug resistance- associated ... surface of the cells, flip-flop across the plasma membrane, accu- mulation of dye in th...
Ngày tải lên: 29/03/2014, 08:20
Báo cáo khoa học: "Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis" pdf
... is to examine the expression of Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis Tae-kyun Shin*, Yong-duk ... in part Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoi...
Ngày tải lên: 07/08/2014, 17:22
Báo cáo khoa học: "Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease" pot
... Agurel@istanbul.edu.tr Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease Burak Kuscu, Aydin G ü rel* Department of Pathology, Faculty of Veterinary Medicine, ... Lesions in the thymus and bone marrow in chicks with CIA 21 Fig. 2. (A) Abundant antigen-specific staining in the cortex a...
Ngày tải lên: 07/08/2014, 20:23
Báo cáo khoa học: "Application of light microscopical and ultrastructural immunohistochemistry in the study of goblet cell carcinoid in the appendix" pdf
... with the hypothesis of the dual ento- dermal and neuroendocrine origin of goblet cell carcinoid [1]. The ultrastructural investigation showed that in mor- phology the endocrine cells were from the ... among the groups of goblet cells infiltrating the submucosa and muscle layer of appendix. Similar clusters of goblet cells and immunorea- tive endocri...
Ngày tải lên: 09/08/2014, 07:21
báo cáo khoa học: " Position of the Phi and Po2 loci in the Hal linkage group in pigs" ppsx
... halothane. Two recombinants (Table 1, Original article Position of the Phi and Po2 loci in the Hal linkage group in pigs P. Vögeli Insfifute of Animal Sciences, Breeding ... informative recombinants between S, Hal, Phi and H on one side and Po2 and Pgd on the other. All five recombinants are informative in determining the...
Ngày tải lên: 09/08/2014, 22:22
Báo cáo khoa học: "Luminal concentrations of L- and D-lactate in the rectum may relate to severity of disease and outcome in septic patients" ppt
... assessment during treatment may be possible [29]. Conclusion Luminal concentrations of L- and D-lactate in the rectum are increased in septic patients and may relate to severity of dis- ease and outcome. ... concentrations of L- and D-lactate in the rectum may relate to severity of disease and outcome in septic patients...
Ngày tải lên: 13/08/2014, 03:20