Báo cáo khoa học: "Site adaptations of Acanthogyrus (Acanthosentis) tilapiae: Observations through light and scanning electron microscopy" pptx

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Báo cáo khoa học: "Multilingual adaptations of ANNIE, a reusable information extraction tool" doc

... summarisa-tion and MT to be carried out in a month ona surprise language, the nature of which willnot be known in advance. The open and flex-ible architecture of GATE, and the separation of linguistic ... Bulgarian named entities in GATE of the gazetteer lists (e.g. lists of first names,days of the week, locations etc. were tailored forBulgarian), and in terms of some of the patternmatching rules ... K. Bontcheva, and V. Tablan. 2002. GATE: A Framework and Graphical Development Environment forRobust NLP Tools and Applications. In Pro-ceedings of the 40th Anniversary Meeting of the Association...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... (Arg21, Arg111, and His138) and with thecarboxylate end (Arg21 and Arg73) of GriP. Of partic-ular note is a short, 2.46-A˚hydrogen bond between N-e of His138 and a phosphate oxygen of GriP.Alanine ... some of the key ﬁndings that have led to our current under-standing of the molecular mode of action of glypho-sate and the molecular basis for glyphosate resistance.Structure and function of ... splaying b4 and b5 strands identiﬁes GLYAT as a member of theGCN5-related N-acetyltransferase superfamily [57].The interactions between cofactor and GLYAT aresimilar to those observed throughout...

Báo cáo khoa học: Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster pdf

... openquestions. The use of mutant (e.g. deletion and extracopy numbers) and transgenic (RNAi and overexpres-sion) lines will help us to better understand therelationship between Hsps and cold tolerance.Experimental ... with$ 10 mL of standard food medium. Flies of the RA groupwere transferred to vials containing $ 10 mL of 1% agar,which provides a source of water but not nutrients. Fordetermination of any cumulative ... stress exposure and recoveryphases. The temporal gene expression of adults was analyzed during 9 h of cold stress at 0 °C and during 8 h of recovery at 25 °C. Increased expres-sion of some, but...

Báo cáo khoa học: NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B–NS3 protease ppt

... site. A NOESY spectrum of 2 and 3 in the presence of a small quantity of proteaserevealed an intermolecular cross-peak between themethyl group of 3 and the phthalazine ring of 2,asexpected for ... spectra of the com-pounds 2 and 3 in the absence and presence of WNVNS2B–NS3pro(K96A) in D2O solution containing1.5% d6-dimethylsulfoxide.Fig. S5. Superimposition of 15N-HSQC spectra of 0.3 ... site of theprotease.In the ﬁrst experiment, we compared the15N-HSQCspectra of WNV NS2B–NS3pro(K96A) in the presence of 3 and in the presence of the Bz-nKRR-H inhibitorused in one of the...

Báo cáo khoa học: Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia pdf

... amixture of annexin A1 and A2 and that the 35 kDa proteins are a mixture of annexin A5 and proteolytic fragments of annexin A1 and annex-in A2. Puriﬁed recombinant annexin A1, annexin A2 and annexin ... show the sites cleaved and the mole-cular masses of the rest of the cleaved peptides. Arrows show theN-termini of the 35 kDa forms of annexin A1 and annexin A2.Role of dicalcin in frog olfactory ... shown asthin dotted lines in (C) and (D). The result obtained in the presence of dicalcin and all of the annexins (E) was compared with the calcu-lated sum of each of the initial rates obtained...

Báo cáo khoa học: Molecular imprinting of cyclodextrin glycosyltransferases from Paenibacillus sp. A11 and Bacillus macerans with c-cyclodextrin pptx

... loss of enzyme activity through imprinting, immo-bilizing and crosslinking of the native enzyme. Thetemperature stability of the immobilized and CLIPCGTases from A11 and BM at 60 °C and 70 ... Pathumthanee, Thailand2 Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Germany3 Starch and Cyclodextrin Research Unit, Department of Biochemistry, ... of syntheticorganic chemistry, biomedical applications, and envi-ronmental catalysis.In this study, the modiﬁcation of the product specif-icity and stability of two CGTases at the level of...

Báo cáo khoa học: Identiﬁcation of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells potx

... position of the absorbance peak (280 nm) of the stand-ard marker with that of aspartyl aminopeptidase.Effect of inhibitors on aspartyl aminopeptidaseactivitySolutions of various inhibitors of enzymatic ... generated was estimated from theratio of the height of the product peptide peak versusthe sum of the heights of the product peptide and sub-strate peaks.Puriﬁcation of yeast aspartyl aminopeptidaseYeast ... bestatin.Yeast aminopeptidase I and aspartyl aminopepti-dase, both of which belong to the M18 family of metal-loprotease, comprise 12 identical subunits, and theactivity of these enzymes is inhibited...

Báo cáo khoa học: Thioredoxin Ch1 of Chlamydomonas reinhardtii displays an unusual resistance toward one-electron oxidation potx

... by a factor of approximately two [25]. T hirdly, a ctivity of D 30A wasweakly modiﬁed only after irradiation w ith 100 Gy, whilethat of WT and of W35A increased by a factor of 1.5 and 1.2, respectively ... Trx, D30A and W35Aafter oxidation with a dose of 100 Gy. For each form, thechromatograms were very similar and showed the forma-tion of a s ingle major product. The y ield of formation of t hisproduct ... one -electron oxidation of wild-type thio-redoxin and of two forms with the point mutations D30A and W35A, using azide radicals generated by c-ray or pulseradiolysis. The oxidation patterns of...

Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-speciﬁc truncated splice variant Mark Wehner and Christian Herrmann doc

... assays,ﬂuorescence titrations and analytical gel ﬁltration.Results and DiscussionHydrolytic activity of hGBP5a/b and hGBP5taIn light of previous observations for hGBP1 and otherlarge GTPases, ... with Hill coefﬁcients of 2.4 and 1.9 and dissoci-ation constants (KdHill) of 4 and 2 lm for hGBP5a ⁄ b and hGBP5ta, respectively. The proteins are very similarin terms of their enzymatic activity, ... enzymatic activity of hGBP5a ⁄ b and hGBP5ta in a concentration-dependentmanner. Various concentrations of puriﬁed hGBP5a ⁄ b and hGBP5ta were incubated with 350 lm of GTP, and aliquots were...

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