Báo cáo khoa học: "Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2" ppsx

... the intracellular segment of the b 1 subunit is required for the interaction with the a subunit, probably a crucial step for the regulation of channel properties. The tissue distribution of the ... I m /I max obtained from the fit. All data points (E, F) correspond to the mean ± SEM of the averaged normalized currents for the number of oocytes indicated. (G) Effe...

Ngày tải lên: 16/03/2014, 23:20

... sequencing of BmKIM cDNA The yield from the initial screening of the cDNA library with the 32 P-labeled cDNA probe was about 260 positive clones. On the final screening, 25 clones were selected on the basis ... work, we describe the cloning of the gene sequence of BmKIM, the functional expression of the recombinant toxin in Escheri- chia coli and its effec...

Ngày tải lên: 31/03/2014, 09:20

... For the rate of decrease of the linear form, the original data were fitted by Y(t) ¼ e –kt · 100%, where Y is the percentage of the linear form, and t is the refolding time. For the rate of increase ... A indicates the oxidase activity, which corresponds to the x-intercept of the RNase activity plot. The oxidase activity matches the slope of the linear plo...

Ngày tải lên: 07/03/2014, 05:20

... cooperates with other activation factors in the WBP for the biosynthesis of long chain rubber molecules. Furthermore, the partial enhancement of HRT2 activity by the addition of the boiled WBP ... analysis of the rubber material synthesized by the action of purified HRT2 in the absence of WBP indicated the production of a small amount of medium chain polyisopr...

Ngày tải lên: 07/03/2014, 21:20

... and USP of insects in other orders and other ecdysozoan are necessary for precise understanding of their physiology. In conclusion, we successfully performed cDNA cloning of EcR and USP of L. decemlineata, ... fumiferana: cloning of cDNA and develop- mental expression of mRNA. Dev Genet 22, 169–179. 16 Imhof MO, Rusconi S & Lezzi M (1993) Cloning of a Chironomus te...

Ngày tải lên: 07/03/2014, 21:20

... identified 5¢ end of the xMGP cDNA [4]. The lower group of bands, identified as site ÔBÕ in Fig. 4, probably corresponds to a premature arrest of the reverse transcriptase due to the presence of an inverted ... [23]. The construction of the )70/)36TATA LUC and 3x()70/)36)TATALUC involved t he cloning of one or three copies of double stranded oligonucleotides spanni...

Ngày tải lên: 08/03/2014, 10:20

... influence the efficacy of interference RNA in vivo, for example, the length of target mRNA, the length and concentration of dsRNA, the region of homology between the dsRNA and the target, as well as other ... to the N-terminal and C-terminal regions of the mature peptide. To obtain the CHH cDNA, we used 10 lg of total eyestalk RNA from adult shrimp by RT-PCR assay...

Ngày tải lên: 23/03/2014, 10:20

... regions (numbers in parentheses indicate the first and last amino acids of the primary sequence of the proteins). The F-region, which exists in the Drosophila EcR and other mammalian nuclear receptors, ... amino acids. The amino acid sequence of the L. australasiae ecdysone receptor was similar to that of the ecdysone receptor-A of the soft tick, Ornithodoros moubat...

Ngày tải lên: 30/03/2014, 04:20

... stage. The study about the nucleotide sequence of canine HIPK2 was performed for the development of cancer therapy because the attack rate of cancer has been increased depending on longevity of pets ... Activation of HIPK2 leads to the selective phosphorylation of p53, resulting in growth arrest and the enhancement of apoptosis. In this study, the canine HIPK2...

Ngày tải lên: 07/08/2014, 18:21

... GmPDIL-3b may be the cause of the lack of such enzymatic activities. Alternatively, the lack of other amino acids, such as arginine, which is important for the regulation of the active site redox ... MM & Gilbert HF (1991) Catalysis of the oxidative folding of ribonuclease A by protein disulfide isomerase: dependence of the rate on the composition of the...

Ngày tải lên: 18/02/2014, 11:20