Báo cáo khoa học: "Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2" ppsx

Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

... the intracellular segment of the b1subunit isrequired for the interaction with the a subunit, probably acrucial step for the regulation of channel properties. The tissue distribution of the ... Im/Imaxobtained from the ﬁt. All data points (E, F) correspond to the mean ± SEM of the averaged normalized currents for the number of oocytes indicated. (G) Eﬀect of b1Bon the current amplitude of NaV1.2. ... expression of the human b1Bsubunitwith NaV1.2 inXenopusoocytesTo explore the regulatory function of the human b1Bsubunit, we injected cRNA of human b1B,aswellascRNA of the sodium...

Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

... sequencing of BmKIM cDNA The yield from the initial screening of the cDNA librarywith the 32P-labeled cDNA probe was about 260 positiveclones. On the ﬁnal screening, 25 clones were selected on the basis ... work, wedescribe the cloning of the gene sequence of BmKIM, the functional expression of the recombinant toxin in Escheri-chia coli and its effect on the sodium channels of neuronsand ventricular ... The yields of afﬁnity-puriﬁed proteins were 10 mgÆL)1 of culture,estimated by Bradford means [36].Enzymatic cleavage of fusion protein and puriﬁcation of the recombinant BmKIM The fusion protein...

Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulﬁde isomerase from Conus marmoreus pdf

... For the rate of decrease of the linear form, the original data were ﬁttedby Y(t) ¼ e–kt· 100%, where Y is the percentage of the linear form, and t is the refolding time. For the rate of increase ... Aindicates the oxidase activity, which corresponds to the x-intercept of the RNase activity plot. The oxidase activitymatches the slope of the linear plot of reciprocal of lag timesagainst the PDI ... experiments.For the rate of increase of the native form in the presence of cPDI, the original data were ﬁtted by Y(t) ¼ Ymax(1 ) e–kt) · 100%, where Y is the percentage of the native form, and t is the...

Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf

... cooperates with other activation factors in the WBPfor the biosynthesis of long chain rubber molecules.Furthermore, the partial enhancement of HRT2 activityby the addition of the boiled WBP ... analysis of the rubber material synthesized by the action of puriﬁedHRT2 in the absence of WBP indicated the production of asmall amount of medium chain polyisoprene intermediates of molecular ... in each of the deduced amino acid sequences encoded by the twocDNAs. In vitro assay of the rubber transferase activity of the recombinant HRT proteins was carried out in the presence of washed...

Báo cáo khoa học: Molecular cloning, expression analysis and functional conﬁrmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf

... and USP of insects in other orders and other ecdysozoanare necessary for precise understanding of theirphysiology.In conclusion, we successfully performed cDNA cloning of EcR and USP of L. decemlineata, ... fumiferana: cloning of cDNA and develop-mental expression of mRNA. Dev Genet 22, 169–179.16 Imhof MO, Rusconi S & Lezzi M (1993) Cloning of aChironomus tentans cDNA encoding a protein (cEcRH)homologous ... degradation products of the full length64 kDa protein. Similar results were obtained for the in vitro translation of USP of C. suppressalis [13].Otherwise, they might be products of internal initiationor...

Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identiﬁes a calcium sensitive region required for basal activity doc

... identiﬁed 5¢ end of the xMGP cDNA [4]. The lower group of bands, identiﬁed as site ÔBÕ in Fig. 4,probably corresponds to a premature arrest of the reversetranscriptase due to the presence of an inverted ... [23]. The construction of the )70/)36TATALUC and 3x()70/)36)TATALUC involved t he cloning of one or three copies of double stranded oligonucleotidesspanning the region from )70 to )36 of t he ... and the )134/)36 bp(lanes 3 a nd 9) fragments from the xMGP gene promoteralmost completely prevented the formation of the DNA- protein complexes (Fig. 7). In contrast, addition of an excessof...

Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

... inﬂuence the efﬁcacy of interference RNA in vivo, for example, the length of target mRNA, the length and concentration of dsRNA, the region of homology between the dsRNA and the target, as well as other ... to the N-terminal and C-terminal regions of the mature peptide.To obtain the CHH cDNA, we used 10 lg of totaleyestalk RNA from adult shrimp by RT-PCR assays. The quality of synthesized cDNA ... according to the instructions of the manufacturer.Northern blot analysisNorthern blot analysis was used to characterize the expres-sion of the shrimp CHH gene. Ten micrograms of eachRNA sample...

Báo cáo khoa học: Molecular cloning of the ecdysone receptor and the retinoid X receptor from the scorpion Liocheles australasiae pot

... regions(numbers in parentheses indicate the ﬁrst and lastamino acids of the primary sequence of the proteins). The F-region, which exists in the Drosophila EcR andother mammalian nuclear receptors, ... amino acids. The amino acid sequence of the L. australasiae ecdysone receptor was similar to that of the ecdysonereceptor-A of the soft tick, Ornithodoros moubata (68%) and to that of the ecdysone ... regions. The C-regions of EcRs andUSPs are highly conserved. However, other regions,particularly the N-terminal parts of USP ⁄ RXR, vary. The alignments of amino acid sequences of the A ⁄ B...

Báo cáo khoa học: "Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2" ppsx

... stage. The study about the nucleotide sequence of canine HIPK2was performed for the development of cancer therapybecause the attack rate of cancer has been increaseddepending on longevity of pets ... Activation of HIPK2 leads to the selectivephosphorylation of p53, resulting in growth arrest and the enhancement of apoptosis. In this study, the canine HIPK2 cDNA fragments were obtained, and theiroverlapping ... and the enhancement of apoptosis[3]. Overexpression of HIPK2 leads to an increase of p53 protein expression or stability [19]. The research of p53 is being conducted to find the mechanisms of...

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

... GmPDIL-3b maybe the cause of the lack of such enzymatic activities.Alternatively, the lack of other amino acids, such asarginine, which is important for the regulation of the active site redox ... MM & Gilbert HF (1991) Catalysis of the oxidative folding of ribonuclease A by protein disulﬁdeisomerase: dependence of the rate on the composition of the redox buffer. Biochemistry 30, 613–619.43 ... a density of 1.21 gÆmL)1in the presence of MgCl2. In the presence of EDTA,which releases ribosomes from the rough ER [24], the peaks of GmPDIL-3a and GmPDIL-3b were shifted to the lighter...

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