Atomic Force Microscopy in Cell Biology Episode 2 Part 5 pptx

... bacteriorhodopsin atomic models werecombinedand compared to the AFM 27 0 M¨uller and Engel region of bacteriorhodopsin, consisting of 25 amino acids, is not observed by AFM and does not in uence the ... F 1 have been solved to atomic resolution (Abrahams et al., 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 26 7 rhodopsin (Rho EF) also interacts with rhodopsi...

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... The AFM Imaging of Living Cells 21 5 2. Henderson, E., Haydon, P. G., and Sakaguchi, D. S. (19 92) Actin filament dy- namics in living glial cells imaged by atomic force microscopy. Science 25 7, 944– 1946. 3. ... illustrating the presence of intra- cytoplasmic vacuoles (as known by correlative TEM studies), 25 µm × 25 µm. 21 6 Braet and Wisse by atomic force microscopy,...

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... proteins in buffer solution at molecular resolution by atomic force microscopy. Biophys. J. 58 , 1 25 1– 1 25 8. 22 . Karrasch, S., Hegerl, R., Hoh, J. H., Baumeister, W., and Engel, A. (1994) Atomic force ... Rev. Sci. Instrum. 61, 3 4 25 –3430. 21 . Gibson, C.T., Watson G. S., and Myhra, S. (1996) Scanning force microscopy- Callibrative procedures for “best practice.” Scan...

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... plasma membrane of a living cell. Scanning occurs in parallel to the longitudinal cell axis. Aldosterone-Sensitive Cells Imaged With AFM 25 5 25 5 19 Shape and Volume of Living Aldosterone-Sensitive Cells Imaged ... (Difco), 5% Giemsa staining solution, and 1X phosphate-buffered saline (PBS). 5. CBG banding: 0 .2 N HCl; 5% Ba(OH) 2 ; 2X SSC buffer; and 5% Giemsa staining so...

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... 1 32, 24 3 25 2. 20 . Marsh, D. J., Jensen, P. K., and Spring, K. R. (19 85) Computer-based determina- tion of size and shape in living cells. J. Microsc. 137, 28 1 29 2. 21 . Timbs, M. M. and Spring, ... Wash cover slips in PBS (4 × 5 min) at 4°C and then fix cells for 15 min in 0 . 25 % glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7 .5. 4. Wash cover slips in PBS...

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... 82, 28 9 29 5. Lehenkari, P. P., and Horton, M. A. (1999). Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy. Biochem. Biophys. Res. Commun. 25 9, 6 45 650 . Lundberg, ... and Yu, L. (20 00). Atomic force microscopy imaging of living cells: A preliminary study of the disruptive effect of the cantilever tip on cell morphology. Ultra...

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... mM NaCl), low-salt MOPS buffer (20 mM MOPS, 25 mM NaCl, 5mM MgCl 2 ), PBS in 5 mM MgCl 2 (10 mM phosphate buffer, 2. 7 mM KCl, 137 mM 22 4 Kindt et al. noncovalent interactions to the substrate and ... studied by single particle tracking (nanovid microscopy) . Effects of calcium-induced differentiation in cultured epithelial cells. Biophys. J. 65( 5), 20 21 20 40. Malmquist, L....

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... than 20 0 nL/min, resulting in volume changes inside the fluid cell of less than ±6 μL in 1 /2 h. A. Principle of Operation Two syringe pumps inject fluid into the AFM fluid cell in an alternating ... U.S.A. 96, 20 01 20 06. Czajkowsky, D. M., and Shao, Z. (1998). Submolecular resolution of single macromolecules with atomic force microscopy. FEBS Lett. 430, 51 54 . 12. Cryo-A...

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... 1 05 111. Perutz, M. F. (19 92) . Protein function below 22 0K. Nature 358 , 54 8. 12. Cryo-AFM 24 9 Fig. 5 Representative cryo-AFM images of single molecules: (a) C1q, (b) IgM, (c) F-actin (inset: actin bundles at ... Soc. 121 , 9 750 –9 751 . Han, W., Mou, J., Sheng, J., Yang, J., and Shao, Z. (19 95) . Cryo atomic force microscopy: A new approach for biological imaging at high r...

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... Appl. Phys. 86, 22 58 22 62. 29 2 M¨uller and Engel future, more complex biological systems will be investigated and may deliver insights into the biogenesis of membrane proteins, into interactions ... for 5 min in acetone and then UV irradiate for 15 min. 2. Incubate cantilever in a 50 -μl drop of biotin–BSA (0 .5 mg/ml in 0.1 M sodium bicarbonate, pH 8.3) overnight at 37 ◦ C...

Ngày tải lên: 06/08/2014, 02:20