In Vitro Maturation of Oocytes doc

... contains serum or human serum albumin. IN VITRO MATURATION OF OOCYTES IN INFERTILITY TREATMENTS The research into maturation of immature oocytes initiated by Pincus and Enzmann (8) and continued ... outcome of patients with polycystic ovary syndrome treated by in vitro maturation and in vitro fertilization–embryo transfer. Fertil Steril 2005; 83:1461–1465. In...

Ngày tải lên: 05/08/2014, 16:20

... During in vitro maturation of por- cine COCs, the addition of EGF to maturation medium significantly elevated developmental competence of oocytes to blastocyst stage [12], indicating that investiga- tion ... suppression of the EGF receptor tyrosine kinase activity by specific inhibitor completely suppressed cumulus expansion and meiotic maturation of oocytes during in...

Ngày tải lên: 20/06/2014, 07:20

... [24], which, in turn, protects the bovine oocytes from oxidative damage. Addition of β -ME to chemical defined maturation medium also increased the percentage of ovine oocytes completing nuclear maturation ... cysteine and cystine. Theriogenology 2000, 53 , 761-771. 27. Matos DG, Gasparrini B, pasqualini SR, Thompson JG. Effect of glutathione synthesis stimulation during in...

Ngày tải lên: 07/08/2014, 18:20

... between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by meas- uring inhibition of the endonuclease reaction [36]. In the ... its binding affinity is not altered by the presence of DNA in the DNA binding site. Interestingly, when 3 n M RNA and 10 n M DNA were added simultaneously to a limiting amount of protei...

Ngày tải lên: 17/03/2014, 10:20

... form of hypophos- phatasia, indicating the importance of bALP in initiating mineral formation [6]. It has been suggested that bALP could be involved in the mineralization process by hydro- lyzing ... femurs in a medium without exogenous inorganic phosphate, but containing calcium and GPI-bALP substrates, served as in vitro model of min- eral formation. The mineralization proce...

Ngày tải lên: 23/03/2014, 17:21

... cells producing TGF-β resulted in the induction of Treg in vivo and in vitro in a murine model [52]. Moreover, the blockade of TGF-β reduces Treg induction by the fusion cell vaccine and enhances ... cells in vitro assay. Methods Cell lines K562 cells (American Type Culture Collection) were maintained in DMEM medium. Colorectal carcinoma cell lines (COLP-2 and COLM-6) were...

Ngày tải lên: 18/06/2014, 15:20

... 5 min of incubation with 0.4% Trypan blue, the percentages of stained cells (indicative of nonviable cells) versus stain-excluding cells were counted in a hemocytometer. Then the percentage of ... 13:95-102. 24. Vincenti MP, Clark IM, Brinckerhoff CE: Using inhibitors of metal- loproteinases to treat arthritis. Easier said than done? Arthri- tis Rheum 1994, 37:1115-1126. 25. Goldr...

Ngày tải lên: 20/06/2014, 04:20

Ngày tải lên: 07/08/2014, 20:23

... also protein fragments for mapping of interaction domains. The use of in vitro expression approaches to analyze protein products is likely to gain momentum for the analysis of known proteins as ... analyzed using in vitro assays. Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique where not only full-length version of pro...

Ngày tải lên: 25/10/2013, 22:20

... NGOs and international organizations. 4.2 Improvement in deriving information 4.2 Improvement in deriving information III. Application of remote sensing III. Application of remote sensing technology ... processes. 3.1 In the past 4.6 Priority needs ) ) Development of Forest Monitoring and Development of Forest Monitoring and Information System (FOMIS) Information System (...

Ngày tải lên: 24/01/2014, 03:20