Aseptic Technique for Cell Culture pptx

... simplified cell culture, particularly for small-scale laboratory needs. BASIC PROTOCOL 1 ASEPTIC TECHNIQUE This protocol describes basic procedures for aseptic technique for the novice in cell culture ... in Cell Biology 1.3.2 Aseptic Technique for Cell Culture Prepare and maintain the work area 6. Perform all aseptic work in a clean work space, free from c...

Ngày tải lên: 11/07/2014, 21:20

... recombinants were assessed for the property of cell- to -cell spread by comparison of plaque sizes. Briefly, MRC-5 cells or MeWo cells were infected with approxi- mately 50 PFU of cell- free viruses of ... mech- anisms of VZV. Methods Cells and viruses MRC-5 cells were cultured with modified minimum essential medium (MEM) supplemented with 10% fetal bovine serum (FBS). MeWo cells were c...

Ngày tải lên: 20/06/2014, 01:20

... described below. After transfection for 6 h, cells were incubated with or without agonists and ⁄ or JHIII for 24 h, cells were harvested, and extracts were processed for the studies. The reagents tested ... text. Extracts from cells grown in culture medium with no added agonist were equalized for gel loading on the basis of b-galactosidase reporter gene activity. Densitometry readin...

Ngày tải lên: 18/02/2014, 08:20

... seed culture of microalgae and C) under continuous fluorescent lamps. Integrated Cultivation Technique for Lipid Production Microbial lipids production via integrated technique was performed ... yeast for used as potential feedstock for biodiesel production and the capture of CO 2 emissions from the yeast fermentation stage [4, 5]. In conclusion, we present a cultivati...

Ngày tải lên: 21/02/2014, 10:20

... selection for certain cell populations such as MSC and decreases the number of CD34 cells [38]. Thus in vitro expanded SVF derived cells can not be compared with primary isolated SVF cells. Immune ... T cells. Stem Cells 2008, 26:1913-1919. 77. Arthur A, Zannettino A, Gronthos S: The therapeutic applica- tions of multipotential mesenchymal/stromal stem cells in skeletal tissue repair. J...

Ngày tải lên: 18/06/2014, 15:20

... Medicine, Clinical Center, NIH for collecting the cells and the Cell Processing Laboratory, Department of Transfusion Medicine, Clinical Center, NIH for preparing the elutriated cells. These studies were ... noncoding B cell integration cluster (Bic) gene [23] and is functionally important in B cell, T cell and macrophage biology. miR-155 is up-regulated in B cells exposed to antig...

Ngày tải lên: 18/06/2014, 16:20

... and obtain sequence information for phylogenetic characterization, gene structure determination and genome analysis is reviewed. While this review describes techniques for the identification ... BioMed Central Page 1 of 24 (page number not for citation purposes) Virology Journal Open Access Review CODEHOP-mediated PCR – A powerful technique for the identification and characterizati...

Ngày tải lên: 18/06/2014, 22:20