hematopoietic stem cell culture ppt
hematopoietic stem cell protocols
... hematopoietic stem cells. Science 241, 58–62.10. Uchida, N. and Weissman, I. L. (1992) Searching for hematopoietic stem cells:evidence that Thy-1.11o Lin- Sca-l+ cells are the only stem cells in ... their propensity to mobilize stem and progenitor cells in response tocytokine administration (23). Recent studies have also demonstrated that stem cell pool size and stem cell cycling characteristics ... (12,28). Therefore, a dose-response experiment Cell Cycle Analysis of Stem Cells 9393From: Methods in Molecular Medicine, vol. 63: Hematopoietic Stem Cell ProtocolsEdited by: C. A. Klug and C....
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Báo cáo hóa học: " Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture" pptx
... mech-anisms of VZV.MethodsCells and virusesMRC-5 cells were cultured with modified minimumessential medium (MEM) supplemented with 10% fetalbovine serum (FBS). MeWo cells were cultured with Dul-becco's ... assessed for the property of cell- to -cell spread by comparison of plaque sizes. Briefly,MRC-5 cells or MeWo cells were infected with approxi-mately 50 PFU of cell- free viruses of rpOka or ... into MRC-5 cells by electroporation using aNucleofection unit (Amaxa biosystems). The cells werethen cultured with MEM supplement with 10% FBS for 4days. To remove BAC sequence, MRC-5 cells were...
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báo cáo hóa học:" Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture" ppt
... AbstractTula hantavirus carrying recombinant S RNA segment (recTULV) grew in a cell culture to the sametiters as the original cell adapted variant but presented no real match to the parental virus. ... recTULVpresents no real match to the original cell adapted variantand that the lower fitness of the recombinant virus can notbe increased by pre-passaging in cell culture. The observedsurvival time ... showed that the lower competitiveness of recTULV could not be increased by pre-passagingin the cell culture. Nevertheless, the recombinant virus was able to survive in the presence of theparental...
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báo cáo hóa học: " Function, Adjustment, Quality of Life and Symptoms (FAQS) in Allogeneic Hematopoietic Stem Cell " potx
... current status of hematopoietic cell transplantation.Annu Rev Med 2003, 54:491-512.6. Horowitz MM: Uses and growth of hematopoietic cell transplantation. InThomas’ hematopoietic cell transplantation ... EA, Cella DF, Beaumont JL, Brady MJ,Horowitz MM, Sobocinski KA, Rizzo JD, Wingard JR: Long-term health-related quality of life, growth, and spiritual well-being after hematopoietic stem- cell ... hematopoietic cell transplantation. Blood 2009, 114(1):7-19.11. Mosher CE, Redd WH, Rini CM, Burkhalter JE, DuHamel KN: Physical,psychological, and social sequelae following hematopoietic stem...
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Tài liệu Review of Chronic Graft- Versus-Host Disease in Children After Allogeneic Stem Cell Transplantation: Nursing Perspective pptx
... disease.Keywordsallogeneic hematopoietic stem cell transplantation, chronic graft-versus-host disease, children, incidence, risk factorsIntroduction Hematopoietic stem cell transplantation (HSCT) ... common after peripheral blood stem cell transplantation than BMT because it is generally accepted that peripheral blood stem cells con-tain greater numbers of infused T-cells (Bishop, 2009; Schmitz ... T cells in graft vs host disease. Journal of Immunol-ogy, 152, 1609-1617.Huang, X., Liu, D., Liu, K., Xu, L., Chen, H., Han, W., . . . Zhang, X. (2009). Haploidentical hematopoietic stem cell...
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Tài liệu New Advances in Stem Cell Transplantation Edited by Taner Demirer ppt
... in mouse embryonic stem cells. Stem Cells 27, 3001-3011. New Advances in Stem Cell Transplantation 14 Fig. 3. Methods for production of induced pluripotent stem cells (iPSCs) by excisable ... carcinoma and embryonic stem cells. Mol Biol Cell 16, 5719-5735. NEW ADVANCES IN STEM CELL TRANSPLANTATION Edited by Taner Demirer New Advances in Stem Cell Transplantation 8 ... the role of stem cells in cell transplantation or regenerative medicine increased remarkably across all sections of the stem cell related journals. The largest number of stem cell articles...
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NGHIÊN CỨU SỬ DỤNG TẾ BÀO GỐC TRUNG MÔ (MESENCHYMAL STEM CELL - MSC) TRONG ĐIỀU TRỊ UNG THƯ ĐẠI - TRỰC TRÀNG TRÊN CHUỘT (SPRAGUE DAWLEY) ppt
... Đại học Huế, tập 73, số 4, năm 2012 NGHIÊN CỨU SỬ DỤNG TẾ BÀO GỐC TRUNG MÔ (MESENCHYMAL STEM CELL - MSC) TRONG ĐIỀU TRỊ UNG THƯ ĐẠI - TRỰC TRÀNG TRÊN CHUỘT (SPRAGUE DAWLEY) Chế Thị Cẩm ... trước khi ứng dụng cấy ghép: Sử dng mỏy FACS Calibur Becton-Dickinson đ (Fluorescence Activated Cell Sorter), với kỹ thuật Flow cytometry cho phép thu thập các thông tin về tế bào thông qua dạng...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
... administration or neural stem cell transplant. A:reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic rat having undergone neural stem cell transplant ... ability of neural stem cells (NSC) to differentiateinto neural cells has been seen in culture [2]. Given thecomplexity of both the structure and function of thecentral nervous system (CNS), it ... neurotrophins in thelesioned micro-environment of the CNS.Materials and methods Culture of Neural stem cellsNeural stem cells were harvested from the cortex of E16Sprague-Dawley rat embryos. The...
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Báo cáo sinh học: "Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction" ppt
... availability of various cellularsources for experimental investigations [6,7,10-15]including bone marrow-derived mesenchymal stem cells(BMDMSCs), hematopoietic stem/ progenitor cells, andcells of embryonic ... adipose-derived mesenchymal stem cells (ADMS Cs). After culturing for 14 days, majority ofisolated adipose-derived stem cells expressing CD29 and CD90 characteristic of mesenchymal stem cells (n = 3). Note ... A,Reisner Y: Transplantation of human hematopoietic stem cells intoischemic and growing kidneys suggests a role in vasculogenesis but nottubulogenesis. Stem Cells 2006, 24:1185-1193.14. Imberti...
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o cáo hóa học:" Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pptx
... purepopulations of these cells for in vitro study of the cellu-lar processes in thalassemic stem cells. While previousattempts have focused on the cell culture system, in thisstudy the phosphoproteome ... induction of apoptosis and the relevantsignaling pathways in hematopoietic stem cells are stillpoorly understood. Hematopoietic stem cells (HSCs)contain specific markers on their surface such ... days, cells were collected for identi-fication of erythroid markers and cell morphology. Cell surface membrane markers were analyzed to confirm cell types of HSCs and erythroid cells. Cell surface...
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- hematopoietic stem cell transplantation thomas
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