for stem cell culture

... predict behavior of stem cells in vivo Overall, microfluidics tools can be used for spatio­ temporal control over the stem- cell microenvironment, so that the ideal ex vivo niche for cell survival and ... microfluidics-based cell- culture systems, and the ease with which fluid flows can be controlled over time and space Human embryonic stem cells (hESCs) are particularly sensitive to handling in culture, ... [3] For cell biologists, the major impact has been in cell separation, single -cell resolution of the dynamics of gene expression, and insights into how mechanical forces applied to individual cells...

Ngày tải lên: 06/08/2014, 19:21

... models for stem- cell research They present a number of advantages, including the fact that a large pool of stem cells is available throughout the lifetime of each individual; the stem- cell pool ... of these remarkable stem cells However, current research in stem- cell biology is providing increasing evidence that the behaviors and fates of stem cells are not inherent, cell- autonomous properties, ... Sánchez Alvarado A: Molecular analysis of stem cells and their descendents during cell turnover and regeneration in the planarian Schmidtea mediterranea Cell Stem Cell 2008, 3:327-339 Pfister D, De...

Ngày tải lên: 06/08/2014, 19:21

... approach therapeutically relevant Cancer stem cells Self-renewal is the hallmark of both stem cells and cancer cells, and in his talk on leukemia stem cells, Weissman addressed this intriguing ... and characterize the cancer stem cell in order to eliminate it It is an exhilarating time for stem- cell research The work presented at the meeting suggested that stem- cell research will lead to ... originate from the transformation of normal stem cells Work in Weissman’s laboratory has established the role of a leukemia stem cell in leukemia If the results hold true for other tumors, the...

Ngày tải lên: 14/08/2014, 14:21

... 1.3.6 Cells 29 1.3.6.1 Immune Cells 29 1.3.6.2 Epithelial Cells 30 1.4 Adult Stem Cells 31 1.4.1 Mammary Stem Cells 32 1.4.1.1 Evidence for ... Neurosphere Culture 57 2.2.3.1 Poly-L-ornithine Coating of Coverslips 57 2.2.4 2.2.5 Culture of Cell Line, MCF-7 58 2.2.6 2.3 Fetal Mesenchymal Stem Cell Culture 58 Methylcellulose ... 127 5.4.1.5 5.5 Mid Density Cell Cultures 128 Methylcellulose Culture of Selected Population 129 5.5.1 Positive Controls for Methylcellulose Cultures 129 5.5.2 In Vitro...

Ngày tải lên: 11/09/2015, 09:57

... Injectable deliverey systems for stem cell therapy 20 2.4.1 Effect of mechanical cues on stem cells 21 2.4.2 Effect of biomolecular cues on stem cells 22 2.4.3 Hydrogels for stem cell therapy 23 2.4.4 ... fabrication and cell seeding 19 2.4 Injectable delivery systems for stem cell therapy Two kinds of approaches are primarily under consideration for stem cell therapies – (i) injection of stem cells with ... various types of stem cells for such stem cell based therapies, few of which are discussed in the following sections Stem cells are broadly classified into embryonic and adult stem cells based on...

Ngày tải lên: 09/09/2015, 11:18

... system can be validly employed to characterize individual insect EcR–USP heterodimers for their responsiveness to agonists and potentiators Utility of the cell culture as a screening assay for ... described below After transfection for h, cells were incubated with or without agonists and ⁄ or JHIII for 24 h, cells were harvested, and extracts were processed for the studies The reagents tested ... receptors from two insect species for which relevant biochemical and in vivo information exists The comparative profiles demonstrate an approach for developing a screening system to identify and characterize...

Ngày tải lên: 18/02/2014, 08:20

... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... of cells It is believed that stem cells also form reservoirs of repair cells to replace cells and tissues that degenerate over the life span of the organism The dual capacity of stem cells for ... stem cell lines, so the ability to share cell lines will create greater access for qualified scientists to participate in human stem cell research A uniform set of criteria for deriving hES cell...

Ngày tải lên: 06/03/2014, 15:20

... blue-staining-negative cells to that of total cells Cells (3 · 105) were seeded on growth medium and counted every day for days of culture The circles indicate the values for wild-type cells and the triangles ... in both wild-type cells and the two Ptb) ⁄ ) ES cell clones Scale bar: 100 lm (B) Cell proliferation assay Cells (5 · 104) were seeded (d0) and counted every day for days of culture The proliferation ... disruption of Ptb in ES cells Ptb) ⁄ flox ES cells were generated by expression of Cre in Ptb) ⁄ flox-hyg ES cells Ptb) ⁄ ) ES cells were generated by infection of Ptb) ⁄ flox ES cells with a retroviral...

Ngày tải lên: 07/03/2014, 00:20

... neural stem cell transplant A: reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic rat having undergone neural stem cell transplant for four ... b-actin (forward: 5′-CTA CAA TGA GCT GCG TGT GG-3′, reverse:5′-CAG TCA GGA TCT TCA TGA GG-3′) The thermo-profile was 50°C for and 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for ... neurotrophins in the lesioned micro-environment of the CNS Materials and methods Culture of Neural stem cells Neural stem cells were harvested from the cortex of E16 Sprague-Dawley rat embryos The...

Ngày tải lên: 18/06/2014, 16:20

... into MRC-5 cells by electroporation using a Nucleofection unit (Amaxa biosystems) The cells were then cultured with MEM supplement with 10% FBS for days To remove BAC sequence, MRC-5 cells were ... recombinants were assessed for the property of cellto -cell spread by comparison of plaque sizes Briefly, MRC-5 cells or MeWo cells were infected with approximately 50 PFU of cell- free viruses of rpOka ... MRC-5 cells Plaque sizes after infection with cell- free rpOka and rpOka∆58 are indicated graphically (upper) and in photographs (lower) MRC-5 cells were infected with each cellfree virus and cultured...

Ngày tải lên: 20/06/2014, 01:20

... miniaturized cell culture chambers for permanent noninvasive characterization of individual cells in a cell layer The miniaturization of the cell culture area guarantees the observation of the complete cell ... polymethylmethacrylate (PMMA) chip as micro cell culture system with a cell culture area of 99 mm2 [32] In comparison to the PMMA-micro cell culture system, the established MCC with its 800-nm-thin ... to the cell culture medium, the suspension cells started to adhere and form neuronal networks (Figure 3c) Mesenchymal stem cells (MSCs) were used as a model for a sensitive in vitro system [43]...

Ngày tải lên: 21/06/2014, 00:20

... Technique for Cell Culture 1.3.2 Current Protocols in Cell Biology Prepare and maintain the work area Perform all aseptic work in a clean work space, free from contaminating air currents and drafts For ... needed Cell Culture 1.3.7 Current Protocols in Cell Biology for aseptic work Laminar-flow cabinets create clean working environments (see below); clean, certified cell lines are available from cell ... steps Aseptic Technique for Cell Culture Laminar-flow cabinets Laminar-flow cabinets or hoods have replaced the open laboratory bench for aseptic work in almost all cell culture and microbiology...

Ngày tải lên: 11/07/2014, 21:20

... without stem cell support Competing interests None declared References 10 11 12 13 14 Marmont AM, van Lint MT, Gualandi F, Bacigalupo A: Autologous marrow stem cell transplantation for severe systemic ... with the same dose of cyclophosphamide without hematopoietic stem cell support, and also to hasten stem cell regeneration of the immune system under the umbrella of the ATG effect Based on the same ... hematopoietic stem cells are not necessary for re-engraftment While intensely immune suppressive, the regimen is not myeloablative and endogenous hematopoiesis would recover The hematopoietic stem cells...

Ngày tải lên: 09/08/2014, 01:23

... hematopoietic stem cell transplantation Hematopoietic stem cell transplantation (HSCT) is a complex multistep procedure involving mobilisation and harvesting of hematopoietic stem cells (HSCs) ... without stem cell rescue has been tested in SLE and scleroderma patients [37-39] The rationale for that procedure is a rapid eradication of autoreactive effector cells while sparing stem cells ... European Group for Blood and Marrow Transplantation, et al.: Autologous stem cell transplantation for systemic lupus erythematosus Lupus 2004, 13:168-176 Saccardi R, Kozak T, Bocelli-Tyndall C,...

Ngày tải lên: 09/08/2014, 10:23

... repeats were performed for each biological replicate: this comprises gels for E6 and gels for E7 For each clone we ran three replicates for t0 (control) and three replicates for t48 hours Corresponding ... processing, we generated a cell bank of cultured mES cells For years, the importance of mES cells to biology and medicine has been attributed both to their ability to proliferate for an indefinite period ... mES cells, this system allows the efficient and long-term overexpression of the transgene in a dose- and time-dependent manner It is therefore suitable for systematic expression of HSA21 cDNAs Cell...

Ngày tải lên: 09/08/2014, 20:22

... information was collected in the Performa and analyzed Viruses were propagated in PBMC derived from buffy coats and cell free viruses stored in liquid nitrogen for subsequent analysis Ghost cell ... prediction and cell culture assay tributing to the evolving diversity of HIV in this region The tropism of these complex viruses based on cell culture experiments was determined to be R5 for 15/17 ... favorably with cell culture for R5 viruses that were predominant in this region These findings suggest that V3 sequence based co-receptor prediction may potentially be an alternate tool to cell culture...

Ngày tải lên: 10/08/2014, 05:21

... for conditioning; Phenytoin for prophylaxis of busulfan’s side affect Tacrolimus; methotrexate and mycophenolate mofetil (0.5 g twice a day on days to +28) for GVHD prophylaxis; Caspofungin for ... doi:10.1186/1756-8722-4-9 Cite this article as: Yu et al.: Allogeneic hematopoietic stem cell transplantation for acute leukemia with Gilbert’s syndrome Journal of Hematology & Oncology 2011 4:9 ... of GS [8,9] List of abbreviations GS: Gilbert’s syndrome; allo-HSCT: Allogeneic hematopoietic stem cell transplantation; AML: acute myeloid leukemia; CR: complete remission; CT: Computed tomography;...

Ngày tải lên: 10/08/2014, 21:23

... techniques for positive selection of CD34 cells by using the CliniMACS system has provided enriched CD34+ stem cells with a powerful and reproducible TCD (median 4.5 logs) in grafts The median cell ... CY/MeCCNU/ATG CSP/MMF/ MTX short 1.8 220 Not done 55% 47% 2-year 19% for SR 31% for HR 12% for SR 39% for HR 2-year 68% for SR 42% for HR Japan, 2006[39] Journal of Hematology & Oncology 2008, 1:27 ... (NK) cell alloreactivity[7-9]; 3) Easy access to repeat donation of donor cells again, when donor-derived cellular therapy is needed for the treatment and/or prophylaxis of relapse, or for second...

Ngày tải lên: 10/08/2014, 22:20

... cell/ Ntot cell × 100, (4) where Npolyg cell is the number of the cells showing the polygonal-like morphology and Ntot cell is the total number of counted cells in each image For the cell adhesion ... water and freeze-dried to obtain the dry weight Tests were performed in water, PBS, cell culture medium and cell culture medium under culture conditions (37°C, 5% CO2) Degradation tests Several ... streptomycin Cells were grown in tissue culture flasks at 37°C in controlled atmosphere (5% CO2) For cell adhesion and proliferation MDCK cells were seeded at a concentration of 104 cells/well...

Ngày tải lên: 11/08/2014, 00:22