... predict behavior of stem cells in vivo Overall, microfluidics tools can be used for spatio temporal control over the stem- cell microenvironment, so that the ideal ex vivo niche forcell survival and ... microfluidics-based cell- culture systems, and the ease with which fluid flows can be controlled over time and space Human embryonic stem cells (hESCs) are particularly sensitive to handling in culture, ... [3] Forcell biologists, the major impact has been in cell separation, single -cell resolution of the dynamics of gene expression, and insights into how mechanical forces applied to individual cells...
... models for stem- cell research They present a number of advantages, including the fact that a large pool of stem cells is available throughout the lifetime of each individual; the stem- cell pool ... of these remarkable stem cells However, current research in stem- cell biology is providing increasing evidence that the behaviors and fates of stem cells are not inherent, cell- autonomous properties, ... Sánchez Alvarado A: Molecular analysis of stem cells and their descendents during cell turnover and regeneration in the planarian Schmidtea mediterranea CellStemCell 2008, 3:327-339 Pfister D, De...
... approach therapeutically relevant Cancer stem cells Self-renewal is the hallmark of both stem cells and cancer cells, and in his talk on leukemia stem cells, Weissman addressed this intriguing ... and characterize the cancer stemcell in order to eliminate it It is an exhilarating time for stem- cell research The work presented at the meeting suggested that stem- cell research will lead to ... originate from the transformation of normal stem cells Work in Weissman’s laboratory has established the role of a leukemia stemcell in leukemia If the results hold true for other tumors, the...
... Injectable deliverey systems forstemcell therapy 20 2.4.1 Effect of mechanical cues on stem cells 21 2.4.2 Effect of biomolecular cues on stem cells 22 2.4.3 Hydrogels forstemcell therapy 23 2.4.4 ... fabrication and cell seeding 19 2.4 Injectable delivery systems forstemcell therapy Two kinds of approaches are primarily under consideration forstemcell therapies – (i) injection of stem cells with ... various types of stem cells for such stemcell based therapies, few of which are discussed in the following sections Stem cells are broadly classified into embryonic and adult stem cells based on...
... system can be validly employed to characterize individual insect EcR–USP heterodimers for their responsiveness to agonists and potentiators Utility of the cellculture as a screening assay for ... described below After transfection for h, cells were incubated with or without agonists and ⁄ or JHIII for 24 h, cells were harvested, and extracts were processed for the studies The reagents tested ... receptors from two insect species for which relevant biochemical and in vivo information exists The comparative profiles demonstrate an approach for developing a screening system to identify and characterize...
... Guidelines for Human Embryonic StemCell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEMCELL RESEARCH Committee on Guidelines for Human Embryonic StemCell Research ... of cells It is believed that stem cells also form reservoirs of repair cells to replace cells and tissues that degenerate over the life span of the organism The dual capacity of stem cells for ... stemcell lines, so the ability to share cell lines will create greater access for qualified scientists to participate in human stemcell research A uniform set of criteria for deriving hES cell...
... blue-staining-negative cells to that of total cells Cells (3 · 105) were seeded on growth medium and counted every day for days of culture The circles indicate the values for wild-type cells and the triangles ... in both wild-type cells and the two Ptb) ⁄ ) ES cell clones Scale bar: 100 lm (B) Cell proliferation assay Cells (5 · 104) were seeded (d0) and counted every day for days of culture The proliferation ... disruption of Ptb in ES cells Ptb) ⁄ flox ES cells were generated by expression of Cre in Ptb) ⁄ flox-hyg ES cells Ptb) ⁄ ) ES cells were generated by infection of Ptb) ⁄ flox ES cells with a retroviral...
... neural stemcell transplant A: reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic rat having undergone neural stemcell transplant for four ... b-actin (forward: 5′-CTA CAA TGA GCT GCG TGT GG-3′, reverse:5′-CAG TCA GGA TCT TCA TGA GG-3′) The thermo-profile was 50°C for and 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for ... neurotrophins in the lesioned micro-environment of the CNS Materials and methods Culture of Neural stem cells Neural stem cells were harvested from the cortex of E16 Sprague-Dawley rat embryos The...
... into MRC-5 cells by electroporation using a Nucleofection unit (Amaxa biosystems) The cells were then cultured with MEM supplement with 10% FBS for days To remove BAC sequence, MRC-5 cells were ... recombinants were assessed for the property of cellto -cell spread by comparison of plaque sizes Briefly, MRC-5 cells or MeWo cells were infected with approximately 50 PFU of cell- free viruses of rpOka ... MRC-5 cells Plaque sizes after infection with cell- free rpOka and rpOka∆58 are indicated graphically (upper) and in photographs (lower) MRC-5 cells were infected with each cellfree virus and cultured...
... miniaturized cellculture chambers for permanent noninvasive characterization of individual cells in a cell layer The miniaturization of the cellculture area guarantees the observation of the complete cell ... polymethylmethacrylate (PMMA) chip as micro cellculture system with a cellculture area of 99 mm2 [32] In comparison to the PMMA-micro cellculture system, the established MCC with its 800-nm-thin ... to the cellculture medium, the suspension cells started to adhere and form neuronal networks (Figure 3c) Mesenchymal stem cells (MSCs) were used as a model for a sensitive in vitro system [43]...
... Technique forCellCulture 1.3.2 Current Protocols in Cell Biology Prepare and maintain the work area Perform all aseptic work in a clean work space, free from contaminating air currents and drafts For ... needed CellCulture 1.3.7 Current Protocols in Cell Biology for aseptic work Laminar-flow cabinets create clean working environments (see below); clean, certified cell lines are available from cell ... steps Aseptic Technique forCellCulture Laminar-flow cabinets Laminar-flow cabinets or hoods have replaced the open laboratory bench for aseptic work in almost all cellculture and microbiology...
... without stemcell support Competing interests None declared References 10 11 12 13 14 Marmont AM, van Lint MT, Gualandi F, Bacigalupo A: Autologous marrow stemcell transplantation for severe systemic ... with the same dose of cyclophosphamide without hematopoietic stemcell support, and also to hasten stemcell regeneration of the immune system under the umbrella of the ATG effect Based on the same ... hematopoietic stem cells are not necessary for re-engraftment While intensely immune suppressive, the regimen is not myeloablative and endogenous hematopoiesis would recover The hematopoietic stem cells...
... hematopoietic stemcell transplantation Hematopoietic stemcell transplantation (HSCT) is a complex multistep procedure involving mobilisation and harvesting of hematopoietic stem cells (HSCs) ... without stemcell rescue has been tested in SLE and scleroderma patients [37-39] The rationale for that procedure is a rapid eradication of autoreactive effector cells while sparing stem cells ... European Group for Blood and Marrow Transplantation, et al.: Autologous stemcell transplantation for systemic lupus erythematosus Lupus 2004, 13:168-176 Saccardi R, Kozak T, Bocelli-Tyndall C,...
... repeats were performed for each biological replicate: this comprises gels for E6 and gels for E7 For each clone we ran three replicates for t0 (control) and three replicates for t48 hours Corresponding ... processing, we generated a cell bank of cultured mES cells For years, the importance of mES cells to biology and medicine has been attributed both to their ability to proliferate for an indefinite period ... mES cells, this system allows the efficient and long-term overexpression of the transgene in a dose- and time-dependent manner It is therefore suitable for systematic expression of HSA21 cDNAs Cell...
... information was collected in the Performa and analyzed Viruses were propagated in PBMC derived from buffy coats and cell free viruses stored in liquid nitrogen for subsequent analysis Ghost cell ... prediction and cellculture assay tributing to the evolving diversity of HIV in this region The tropism of these complex viruses based on cellculture experiments was determined to be R5 for 15/17 ... favorably with cellculturefor R5 viruses that were predominant in this region These findings suggest that V3 sequence based co-receptor prediction may potentially be an alternate tool to cell culture...
... for conditioning; Phenytoin for prophylaxis of busulfan’s side affect Tacrolimus; methotrexate and mycophenolate mofetil (0.5 g twice a day on days to +28) for GVHD prophylaxis; Caspofungin for ... doi:10.1186/1756-8722-4-9 Cite this article as: Yu et al.: Allogeneic hematopoietic stemcell transplantation for acute leukemia with Gilbert’s syndrome Journal of Hematology & Oncology 2011 4:9 ... of GS [8,9] List of abbreviations GS: Gilbert’s syndrome; allo-HSCT: Allogeneic hematopoietic stemcell transplantation; AML: acute myeloid leukemia; CR: complete remission; CT: Computed tomography;...
... techniques for positive selection of CD34 cells by using the CliniMACS system has provided enriched CD34+ stem cells with a powerful and reproducible TCD (median 4.5 logs) in grafts The median cell ... CY/MeCCNU/ATG CSP/MMF/ MTX short 1.8 220 Not done 55% 47% 2-year 19% for SR 31% for HR 12% for SR 39% for HR 2-year 68% for SR 42% for HR Japan, 2006[39] Journal of Hematology & Oncology 2008, 1:27 ... (NK) cell alloreactivity[7-9]; 3) Easy access to repeat donation of donor cells again, when donor-derived cellular therapy is needed for the treatment and/or prophylaxis of relapse, or for second...
... cell/ Ntot cell × 100, (4) where Npolyg cell is the number of the cells showing the polygonal-like morphology and Ntot cell is the total number of counted cells in each image For the cell adhesion ... water and freeze-dried to obtain the dry weight Tests were performed in water, PBS, cellculture medium and cellculture medium under culture conditions (37°C, 5% CO2) Degradation tests Several ... streptomycin Cells were grown in tissue culture flasks at 37°C in controlled atmosphere (5% CO2) Forcell adhesion and proliferation MDCK cells were seeded at a concentration of 104 cells/well...
... understood Cell fusion, in which stem cells fuse with the somatic cell in the niche, had been suggested by many authors [11] and strong evidences were reported for the transdifferentiation of stem cells ... transplantation of stem cells are augmented by the delivery of bioactive molecules that induce stem cells to differentiate into specific-lineage cells HGF has been reported to be a potent agent for acceleration ... of activated hepatic stellate cells by mesenchymal stem cells Biochem Biophys Res Commun 2007, 363:247-252 37 Kallis YN, Alison MR, Forbes SJ: Bone marrow stem cells and liver disease Gut 2007,...