... sip(ba) mutants. Aliquots (5 0 lL) o f cell-freesupernatant of 24 h TBY-cultures of wild type GBA12 (1 ) mutantstrains sipS(ba) GBA13 (2 ), sipT(ba)GBA1 4(3 ), sipV(ba)GBA1 5(4 ) and sipW(ba) GBA 16 (5 ) ... typeGBA12; (s), sipS(ba) mutant GBA13; (. ), sipT(ba) GBA14; (, ), sipV(ba) mutant GBA 15; (j) sipW(ba) mutant GBA16. (B) SDS/PAGE separation of the CWBP fraction of B. amyloliquefaciensGBA12 and sipV(ba) ... IT41cultures, but not with SipV(ba)orSipW(ba) expression(Fig. 3). Sip disruption mutantsIn order to s tudy the phenotype of sipS(ba), sip T(ba),sipV(ba) and sipW(ba) mutants, B. amyloliquefaciensstrains...