Probiotic Lactobacillus Strains: in vitro and in vivo Studies pot
... their immunomodulating properties in in vivo mouse model. MATERIAL AND METHODS Survival of Lactobacillus strains at low pH and in the presence of bile salt. Lactobacillus strains isolated from ... transit into the gut, and modulate T H 1–T H 2 balance in favor of anti-allergic T H 1 immune responses. In this study we report a selection of probiotic Lactobacillus...
Ngày tải lên: 07/07/2014, 19:20
... gentamicin (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine, 5 lgÆmL )1 insulin (Gibco Invitrogen), 10 ngÆmL )1 epidermal growth factor (Gibco Invitrogen) and 240 lgÆmL )1 Geneticin Ò (G418; Gibco Invitrogen). ... Gibco Invitrogen, Carlsbad, CA, USA) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen)...
Ngày tải lên: 18/02/2014, 08:20
... DNA binding and chromatin association of ATR (ataxia telangiecta- sia-mutated and Rad3-related) in vitro via ATR inter- acting protein [4,22,23]. Rad17 and Rad9 complexes (Rad17–RFC2–5 and Rad9–Rad1–Hus1) ... protein. J Biol Chem 272, 7940–7945. 16 Golub EI, Gupta RC, Haaf T, Wold MS & Radding CM (1998) Interaction of human rad51 recombination protein with single-stranded DNA...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... shown). In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing ... after 0, 30 and 60 min digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane prot...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... O-chain containing L -rhamnose, 2-amino-2-deoxy- D -mannose and D -glucose [11], confirming that the novel CPS and O-chain polysaccharide were produced during the 72-h in vivo culture p eriod. In ... consistent with its previously determined composition of a trisaccha- ride repeating unit consisting of Qui3NAlaNAc, GalN- AcAN and QuiNAc and confirming its presence in t he in...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
... the protein. The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23]. In vitro self-cleavage was also observed in all investigated bacterial SPase I including enzymes ... and protease inhibitor cocktail, and boiling for 10 min to protect proteins from nonspecific proteolysis. S. pneumoniae cells maintain the full length SPase I in the highest leve...
Ngày tải lên: 23/03/2014, 21:21
Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot
... of binding of the fluorescent probe is given as extent of carbohydrate-ligand-dependent binding by subtracting carbohydrate-independent binding from total binding for each cell line. Cell line BiB10-BSA ... the neoglycoproteins, we determined their binding to cells and to tissue sections as well as their biodistribution in mice after injection of radioiodinated material. Binding to cells...
Ngày tải lên: 30/03/2014, 13:20
Báo cáo hóa học: " Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug " pot
... but repeatable increase in the efficacy of N-methanocarbathy- midine (N-MCT) (Table 2). This compound is a carbocy- clic thymidine analog that inhibits the replication of VV both in vitro and in vivo [12,13], ... animal models including route of infec- tion. In HSV, direct intracranial inoculation resulted in a 10-fold reduction in virulence of the mutant, whereas footpad in...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo hóa học: " N-methylisatin-beta-thiosemicarbazone derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection in vitro and in vivo" ppt
... replication. The therapeutic potential of SCH 16 against JEV was eval- uated in vivo in mice using the intracerebral and intraperi- toneal challenge studies. The mice that were evaluated in the intracerebral ... reports describing the in vitro inhibitory effect of compounds such as ribavirin, mycophenolic acid, imino sugars, inhibitors of serine protease, RNA interference and...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo hóa học: " Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA" pot
... report Inhibition of foot -and- mouth disease virus replication in vitro and in vivo by small interfering RNA Wang Pengyan, Ren Yan, Guo Zhiru* and Chen Chuangfu* Address: College of Animal Science and ... subcutaneous injection of siRNA-expressing plasmids in the neck of the suckling mice made them less susceptible to infection with O, and Asia I serotypes of FMDV. Findings...
Ngày tải lên: 20/06/2014, 01:20