Introduction to Modern Liquid Chromatography, Third Edition part 31 pdf
Introduction to Modern Liquid Chromatography, Third Edition part 31 pdf
... the
interface between the stationary and mobile phases. Partition (Fig. 6.4c) considers
the stationary phase to be similar to a liquid phase, into which the solute molecule
dissolves. Notice that ... back onto or into the
stationary phase—rather than extending into the mobile phase as shown.
Presumably there is a decreased interaction with the column for solute
molecules that are too lo...
Introduction to Modern Liquid Chromatography, Third Edition part 95 pdf
... rules, 583–584
structure vs. chromatography, 583–584
Proteomics, multidimensional liquid
chromatography, 616–618
Protocol; see Validation, protocol
Pseudocritical chromatography, 654
Pump(s), 104–113
active ... U-HPLC
High-pressure liquid chromatography, 8
High-priced liquid chromatography; see
U-HPLC
High-speed liquid chromatography, 8
HILIC, see Hydrophilic interaction
chr...
Introduction to Modern Liquid Chromatography, Third Edition part 1 pdf
... Hybrid Systems, 111
INTRODUCTION TO
MODERN LIQUID
CHROMATOGRAPHY
Third Edition
LLOYD R. SNYDER
LC Resources, Inc.
Orinda, CA
JOSEPH J. KIRKLAND
Advanced Materials Technology
Wilmington, DE
JOHN W. ... Lloyd R.
Introduction to modern liquid chromatography / Lloyd R. Snyder, Joseph J. Kirkland. – 3rd
ed. / John W. Dolan.
p. cm.
Includes index.
ISBN 978-0-470-16754-0 (cloth)...
Introduction to Modern Liquid Chromatography, Third Edition part 4 pdf
... interaction chromatography
HILIC hydrophilic interaction chromatography
i.d. column or tubing inner diameter (mm)
IEC ion-exchange chromatography
IPC ion-pair chromatography
k retention factor (same ... retention factor (same as capacity factor k
); equal to (t
R
/t
0
) − 1
k
∗
gradient retention factor; Equation (9.5)
L column length (mm)
LC-MS liquid chromatography–mass spectrometry
L...
Introduction to Modern Liquid Chromatography, Third Edition part 16 pdf
... Accumulator-Piston Pumps
An alternative design for the dual-piston pump is the accumulator-piston, or
tandem-piston design shown in Figure 3.13d. In this case, one piston feeds into
the other piston ... with either the dual-piston or accumulator-piston
design.
3.5.1.3 Active Check Valve
A final refinement in design can be applied to both the dual-piston and
accumulator-piston pump designs. Th...
Introduction to Modern Liquid Chromatography, Third Edition part 17 pdf
... cycle time to just a few seconds.
Three common autosampler designs are in common use:
• pull -to- fill
• push -to- fill
• needle-in-loop
3.6.2.1 Pull -to- Fill Autosamplers
The pull -to- fill autosampler ... detector. One way to avoid this is to use a
switching valve to divert to waste the portion of the column effluent that contains
most of the protein—usually the early portion of the...
Introduction to Modern Liquid Chromatography, Third Edition part 19 pdf
... operator’s
manual, replacement of the needle seal every six months will help to avoid problems.
The autosampler is the component of the HPLC system that is most likely to leak; it
is wise to inspect ... proportioning-manifold replacement, injector rotor,
detector lamp, tubing and fittings
Factory-trained Proportioning-manifold repair, electronics, repairs involving module
subassemblies,...
Introduction to Modern Liquid Chromatography, Third Edition part 21 pdf
... magnetic
resonance
Radioactivity
Conductivity
Chemiluminescent
nitrogen
Chiral
4.3 INTRODUCTION TO INDIVIDUAL DETECTORS
The remainder of this chapter (Sections 4.4–4.16) provides a discussion of most
HPLC detectors in use today. In Table 4.4, detectors are grouped ... 220, 280, 313 , 334, and 365 nm can be accomplished with
the fixed-wavelength detector.
4.4.2 Variable-Wavelength Detector...
Introduction to Modern Liquid Chromatography, Third Edition part 52 pdf
... software that facilitates method development. In this chapter we will
Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder,
Joseph J. Kirkland, and John W. Dolan
Copyright ... Waeghe, J. Chromatogr., 803 (1998) 1.
43. A. P. Schellinger, D. R. Stoll, and P. W. Carr, J. Chromatogr. A, 1064 (2005) 143.
44. A. P. Schellinger, D. R. Stoll, and P. W. Carr, J....
Introduction to Modern Liquid Chromatography, Third Edition part 65 pdf
... selectivity relative to other modes of col-
umn chromatography. Three other chromatographic techniques (chromatofocusing,
hydroxyapaptite chromatography, and immobilized-metal affinity chromatography;
Sections ... point, binding to an anion
exchanger below the protein pI, or binding to a cation exchanger above the pI.
Similarly a protein may fail to bind to an anion exchanger above...
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