Introduction to Modern Liquid Chromatography, Third Edition part 16 pdf

... Accumulator-Piston Pumps An alternative design for the dual-piston pump is the accumulator-piston, or tandem-piston design shown in Figure 3.13d. In this case, one piston feeds into the other piston ... with either the dual-piston or accumulator-piston design. 3.5.1.3 Active Check Valve A final refinement in design can be applied to both the dual-piston and accumulator-piston pump designs. Th...

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... rules, 583–584 structure vs. chromatography, 583–584 Proteomics, multidimensional liquid chromatography, 616 618 Protocol; see Validation, protocol Pseudocritical chromatography, 654 Pump(s), 104–113 active ... U-HPLC High-pressure liquid chromatography, 8 High-priced liquid chromatography; see U-HPLC High-speed liquid chromatography, 8 HILIC, see Hydrophilic interaction chr...

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... Lloyd R. Introduction to modern liquid chromatography / Lloyd R. Snyder, Joseph J. Kirkland. – 3rd ed. / John W. Dolan. p. cm. Includes index. ISBN 978-0-470 -167 54-0 (cloth) 1. Liquid chromatography. ... Hybrid Systems, 111 INTRODUCTION TO MODERN LIQUID CHROMATOGRAPHY Third Edition LLOYD R. SNYDER LC Resources, Inc. Orinda, CA JOSEPH J. KIRKLAND Advanced Materials Tec...

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... interaction chromatography HILIC hydrophilic interaction chromatography i.d. column or tubing inner diameter (mm) IEC ion-exchange chromatography IPC ion-pair chromatography k retention factor (same ... retention factor (same as capacity factor k  ); equal to (t R /t 0 ) − 1 k ∗ gradient retention factor; Equation (9.5) L column length (mm) LC-MS liquid chromatography–mass spectrometry L...

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... cycle time to just a few seconds. Three common autosampler designs are in common use: • pull -to- fill • push -to- fill • needle-in-loop 3.6.2.1 Pull -to- Fill Autosamplers The pull -to- fill autosampler ... detector. One way to avoid this is to use a switching valve to divert to waste the portion of the column effluent that contains most of the protein—usually the early portion of the...

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... operator’s manual, replacement of the needle seal every six months will help to avoid problems. The autosampler is the component of the HPLC system that is most likely to leak; it is wise to inspect ... proportioning-manifold replacement, injector rotor, detector lamp, tubing and fittings Factory-trained Proportioning-manifold repair, electronics, repairs involving module subassemblies,...

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... magnetic resonance Radioactivity Conductivity Chemiluminescent nitrogen Chiral 4.3 INTRODUCTION TO INDIVIDUAL DETECTORS The remainder of this chapter (Sections 4.4–4 .16) provides a discussion of most HPLC detectors in use today. In Table 4.4, detectors are grouped ... be found in [16, 17]. 4.4 UV-VISIBLE DETECTORS The most widely used detectors in modern HPLC are photometers based on u...

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... the interface between the stationary and mobile phases. Partition (Fig. 6.4c) considers the stationary phase to be similar to a liquid phase, into which the solute molecule dissolves. Notice that ... back onto or into the stationary phase—rather than extending into the mobile phase as shown. Presumably there is a decreased interaction with the column for solute molecules that are too lo...

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... software that facilitates method development. In this chapter we will Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder, Joseph J. Kirkland, and John W. Dolan Copyright ... Chromatogr. A, 1107 (2006) 139. 53. D. R. Stoll, X. Li, X. Wang, P. W. Carr, S. E. G. Porter, and S. C. Rutan, J. Chromatogr. A, 1168 (2007) 3. 54. M. Gilar and U. D. Neue, J. Chr...

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... selectivity relative to other modes of col- umn chromatography. Three other chromatographic techniques (chromatofocusing, hydroxyapaptite chromatography, and immobilized-metal affinity chromatography; Sections ... point, binding to an anion exchanger below the protein pI, or binding to a cation exchanger above the pI. Similarly a protein may fail to bind to an anion exchanger above...

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