Introduction to Modern Liquid Chromatography, Third Edition part 4 pdf
Introduction to Modern Liquid Chromatography, Third Edition part 4 pdf
... Retention-Problem
Symptoms, 836
17 .4. 4 Peak Area, 838
17 .4. 4.1 Peak Area Too Large, 839
17 .4. 4.2 Peak Area Too Small, 840
17 .4. 4.3 Peak Area Too Variable, 840
17 .4. 5 Other Problems Associated with the
Chromatogram, ... Column Leaks, 828
17 .4. 1.6 Detector Leaks, 828
17 .4. 2 Abnormal Pressure, 830
17 .4. 2.1 Pressure Too High, 831
17 .4. 2.2 Pressure Too Low, 832
17 .4. 2.3 Press...
Introduction to Modern Liquid Chromatography, Third Edition part 95 pdf
... 47 0
initial %B, 41 9 42 0
initial separation, 43 7 44 2
irregular samples, 41 4 41 5, 42 8 43 0
vs. isocratic elution, 40 4 40 5, 40 9 41 3,
43 7 44 0
large molecules, 46 4 46 5
late elution, 42 1, 44 1
linear gradients ... practice),
815
problems, 44 0 44 2, 47 0; see also
Troubleshooting
steep gradients, 860–861
program, 40 9
range, 40 7 40 8, 41 0, 44 4 44 5
regular sampl...
Introduction to Modern Liquid Chromatography, Third Edition part 1 pdf
... Lloyd R.
Introduction to modern liquid chromatography / Lloyd R. Snyder, Joseph J. Kirkland. – 3rd
ed. / John W. Dolan.
p. cm.
Includes index.
ISBN 978-0 -47 0-167 54- 0 (cloth)
1. Liquid chromatography. ... 63
2.5 .4 Method Development, 65
2.5 .4. 1 Assessment of Sample
Composition and Separation
Goals, 65
2.5 .4. 2 Sample Pretreatment, 66
2.5 .4. 3 Selection of Chromatographic
Mo...
Introduction to Modern Liquid Chromatography, Third Edition part 16 pdf
... Accumulator-Piston Pumps
An alternative design for the dual-piston pump is the accumulator-piston, or
tandem-piston design shown in Figure 3.13d. In this case, one piston feeds into
the other piston ... with either the dual-piston or accumulator-piston
design.
3.5.1.3 Active Check Valve
A final refinement in design can be applied to both the dual-piston and
accumulator-piston pump designs. Th...
Introduction to Modern Liquid Chromatography, Third Edition part 17 pdf
... cycle time to just a few seconds.
Three common autosampler designs are in common use:
• pull -to- fill
• push -to- fill
• needle-in-loop
3.6.2.1 Pull -to- Fill Autosamplers
The pull -to- fill autosampler ... 5 (<1) 30 (3)
50 4. 6 3.0 5,500 40 (4) 80 (10) 565 (60)
50 2.1 3.0 5,500 8 (1) 20 (2) 120 (80)
50 1.0 3.0 5,500 2 (<1) 5 (<1) 25 (3)
50 4. 6 1.8 9,200 30 (4) 65 (7) 44 0 (50)...
Introduction to Modern Liquid Chromatography, Third Edition part 19 pdf
... operator’s
manual, replacement of the needle seal every six months will help to avoid problems.
The autosampler is the component of the HPLC system that is most likely to leak; it
is wise to inspect ... checked for leaks.
REFERENCES 145
3. J. N. Brown, M. Hewins, J. H. M. van der Linden, and J. H. M Lynch, J. Chromatogr.,
2 04 (1981) 115.
4. L. R. Snyder, J. Chromatogr. Sci., 21 (198...
Introduction to Modern Liquid Chromatography, Third Edition part 21 pdf
... value.
160 DETECTION
Table 4. 4
HPLC Detectors
Sample-Specific
(Sections 4. 4 4. 10)
Bulk Property
(Sections 4. 11 4. 13)
Hyphenated
(Sections 4. 14 4. 15)
Reaction
(Section 4. 16)
UV-visible Refractive ... magnetic
resonance
Radioactivity
Conductivity
Chemiluminescent
nitrogen
Chiral
4. 3 INTRODUCTION TO INDIVIDUAL DETECTORS
The remainder of this chapter (Sections 4. 4 4. 16) provi...
Introduction to Modern Liquid Chromatography, Third Edition part 31 pdf
... CHROMATOGRAPHY FOR NEUTRAL SAMPLES
80% ACN
(k = 0.3)
R
s
= 0.0
(a)
(b)
(c)
1
2
3
4
1
2
3
4
0.2 0 .4 0.6 0.8
Time (min)
t
0
1 4
50% ACN
R
s
= 1.0
(1.9 ≤ k ≤ 4. 2)
40 % ACN
R
s
= 1 .4
(4 ≤ k ≤ 10)
0 4
Time ... into the stationary phase, but is retained at the
interface between the stationary and mobile phases. Partition (Fig. 6.4c) considers
the stationary phase to be similar to...
Introduction to Modern Liquid Chromatography, Third Edition part 52 pdf
... Chromatogr., 803 (1998) 1.
43 . A. P. Schellinger, D. R. Stoll, and P. W. Carr, J. Chromatogr. A, 10 64 (2005) 143 .
44 . A. P. Schellinger, D. R. Stoll, and P. W. Carr, J. Chromatogr. A, 1192 (2008) 41 .
45 . ... we will
Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder,
Joseph J. Kirkland, and John W. Dolan
Copyright © 2010 John Wiley & So...
Introduction to Modern Liquid Chromatography, Third Edition part 65 pdf
... 8.8–10.8 6.8–7.9
Arginyl 12.5 ≥12
Histidyl 6.0 6 .4 7 .4
Lysyl 10.8 5.9–10 .4
Terminal carboxyl 1.8–2.6 3.5 4. 3
Aspartyl 3.9 4. 0–7.3
Glutamyl 4. 3 4. 0–7.3
Source: Reprinted from [37] with permission ... ion-exchange chromatography.
13 .4. 2.3 Chromatofocusing
Chromatofocusing is a specialized form of IEC in which proteins are eluted from
the column with a pH gradient [44 49 ]. Chromat...
Từ khóa: