Introduction to Modern Liquid Chromatography, Third Edition part 2 potx
Introduction to Modern Liquid Chromatography, Third Edition part 2 potx
... 199 5.1 Introduction, 20 0 5 .2 Column Supports, 20 0 5 .2. 1 Particle Characterization, 20 1 5 .2. 1.1 Particle Type, 20 1 5 .2. 1 .2 Particle Size and Pore Diameter, 20 3 5 .2. 2 Silica Supports, 20 3 5 .2. 2.1 ... Efficiency, 20 5 5 .2. 2 .2 Nature of the Silica Surface, 20 8 5 .2. 2.3 Particle Preparation, 21 1 5 .2. 3 Porous Polymers, 21 2 5 .2. 4 Monoliths, 21 2 5 .2. 4.1...
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Introduction to Modern Liquid Chromatography, Third Edition part 12 potx
... Equations (2. 7) and (2. 10a): V p0 ≈ 0.002Ld c 2 N −0.5 (1 + k) (2. 28) 1 3 4 5 67 2 Time (min) Figure 2. 22 Effect of sample volume V s on peak width and shape. V s /V c = 0.3 (peak 1); 3 (peak 2) ; 5 ... 1 to 4, the peak begins to widen and then develops a flat top. For Equation (2. 27) and the examples of Figure 2. 22, we assume the delivery of an undistorted (i.e., cylindrical...
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Introduction to Modern Liquid Chromatography, Third Edition part 22 potx
... the analytical requirements. 166 DETECTION 21 0 22 0 23 0 24 0 25 0 26 0 27 0 28 0 Wavelength (nm) Absorbance (b) X Y X (26 0 nm) Y (26 0 nm) X + Y (26 0 nm) (a) Figure 4. 12 Illustration of spectral deconvolution ... see [20 , 21 ] or the detector manufacturer’s literature (e.g., [22 ]). 4.4.4 General UV-Detector Characteristics Table 4.5 summarizes the general characteristics of U...
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Introduction to Modern Liquid Chromatography, Third Edition part 29 potx
... H 1 )] 2 + [100(S ∗ 2 − S ∗ 1 )] 2 + [30(A 2 − A 1 )] 2 + [143(B 2 − B 1 )] 2 + [83(C 2 − C 1 )] 2 } 0.5 (5.4) Values of H 1 and H 2 refertovaluesofH for columns 1 and 2, and similarly for the remaining ... the particles are freely dispersed and nonaggregated, as illustrated by the cartoon of Figure 5 .25 a, the liquid may be acceptable. However, if the particles tend...
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Introduction to Modern Liquid Chromatography, Third Edition part 58 potx
... Swartz 12. 1 INTRODUCTION, 5 32 12. 2 TERMS AND DEFINITIONS, 534 12. 2.1 Accuracy, 535 12. 2 .2 Precision, 536 12. 2.3 Specificity, 539 12. 2.4 Limit of Detection and Limit of Quantification, 539 12. 2.5 Linearity ... and Range, 540 12. 2.6 Robustness, 540 12. 3 SYSTEM SUITABILITY, 5 42 12. 4 DOCUMENTATION, 543 12. 4.1 Validation Protocol, 544 12. 4 .2 Test Method, 544 12. 4.3 V...
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Introduction to Modern Liquid Chromatography, Third Edition part 83 potx
... exchange (weak) Amino, 1 ◦ ,2 ◦ -amino (–CH 2 –) 3 NH 2 , (–CH2–) 3 NHCH 2 CH 2 NH 2 Ionic (ionizable), acidic Water or buffer (pH = pK a + 2) A. Buffer (pH = pK a − 2) 7 82 776 SAMPLE PREPARATION • ... as a poly-dimethylsiloxane or a polyacrylate [ 32, 33]. The fiber is dipped into the solution to be analyzed, and analytes diffuse to and partition into the coating as a fu...
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Introduction to Modern Liquid Chromatography, Third Edition part 90 potx
... stream due to partially blocked column-inlet frit. (c) Distortion of sample stream due to void at head of column. 0 426 8 121 0 Time (min) (a) (b) Figure 17.19 Split peaks attributed to a partially ... way to flush particulate matter from the top of the inlet frit and restore normal peak shapes. After reversing the column, flow 20 to 30 mL of solvent through the column to waste (no...
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Introduction to Modern Liquid Chromatography, Third Edition part 95 pdf
... group, 22 6 22 7 functionality, 22 5 22 7 ‘‘horizontal’’ vs. ‘‘vertical’’ polymerization, 21 9 22 0 monomeric, 21 8 21 9, 23 2 23 4 phenyl, 22 6 polymeric, 21 8 21 9, 22 1 22 2, 23 2 23 4 preparation, 21 8 22 5 selectivity; ... 376–385 optimization, 59–61 pH, 320 – 322 RPC, 26 3 28 4, 320 – 327 shape, 23 2 23 5 solvent-strength, 322 – 323 NPC, 376 and solvent-type, 29 0 29 2 a...
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Introduction to Modern Liquid Chromatography, Third Edition part 1 pdf
... CONTROL OF SEPARATION 19 2. 1 Introduction, 20 2. 2 The Chromatographic Process, 20 2. 3 Retention, 24 v vi CONTENTS 2. 3.1 Retention Factor k and Column Dead-Time t 0 ,25 2. 3 .2 Role of Separation Conditions ... 73 2. 6.3 .2 Trace Analysis, 73 2. 7 RELATED TOPICS, 74 2. 7.1 Column Equilibration, 74 2. 7 .2 Gradient Elution, 75 2. 7.3 Peak Capacity and Two-dimensional Sepa...
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Introduction to Modern Liquid Chromatography, Third Edition part 3 ppsx
... Introduction, 5 32 12. 2 Terms and Definitions, 534 12. 2.1 Accuracy, 535 12. 2 .2 Precision, 536 12. 2 .2. 1 Repeatability, 536 12. 2 .2. 2 Intermediate Precision, 537 12. 2 .2. 3 Reproducibility, 537 12. 2 .2. 4 Ruggedness, ... Considerations, 728 15 .2 Equipment for Prep-LC Separation, 730 15 .2. 1 Columns, 730 15 .2. 2 Sample Introduction, 731 15 .2. 2.1 Loop Injectors, 731 15...
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