Introduction to Modern Liquid Chromatography, Third Edition part 1 pdf
Introduction to Modern Liquid Chromatography, Third Edition part 1 pdf
... ABBREVIATIONS xxxv 1 INTRODUCTION 1 1 .1 Background Information, 2 1. 1 .1 What Is HPLC?, 2 1. 1.2 What Can HPLC Do?, 4 1. 2 A Short History of HPLC, 6 1. 3 Some Alternatives to HPLC, 8 1. 3 .1 Gas Chromatography ... Special Forms of HPLC, 12 1. 4 Other Sources of HPLC Information, 12 1. 4 .1 Books, 12 1. 4.2 Journals, 13 1. 4.3 Reviews, 13 1. 4.4 Short Courses, 13...
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Introduction to Modern Liquid Chromatography, Third Edition part 95 pdf
... see also Autosamplers accuracy, 11 5 filled-loop, 11 4, 11 7, 11 8 laminar flow, 11 5 operation, 11 4 partial-loop, 11 5, 11 8 Inlet-line frit, 90 In-line filter, 10 3 Inorganic particles, 214 – 217 Installation ... limits drift, 15 3–55 electrochemical, 17 0 17 2 error sources, 509 flow cell, 15 0, 16 1 fluorescence, 16 7 17 0 quenching, 17 0 FTIR, 19 1 19 2 heat exchangers, 15...
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Introduction to Modern Liquid Chromatography, Third Edition part 4 pdf
... Leaks, 825 17 .4 .1. 4 Autosampler Leaks, 825 17 .4 .1. 5 Column Leaks, 828 17 .4 .1. 6 Detector Leaks, 828 17 .4.2 Abnormal Pressure, 830 17 .4.2 .1 Pressure Too High, 8 31 17.4.2.2 Pressure Too Low, 832 17 .4.2.3 ... CONTENTS 17 .3.5 Module Substitution, 820 17 .3.6 Put It Back, 8 21 17.4 Common Symptoms of HPLC Problems, 8 21 17.4 .1 Leaks, 822 17 .4 .1. 1 Pre-pump Leaks, 822...
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Introduction to Modern Liquid Chromatography, Third Edition part 16 pdf
... today. 3.5 .1. 2 Accumulator-Piston Pumps An alternative design for the dual-piston pump is the accumulator-piston, or tandem-piston design shown in Figure 3 .13 d. In this case, one piston feeds into the ... used to compensate for pump pulsations; today, the need for mechanical pulse damping is due to low-pulse pump designs (e.g., dual-piston or accumulator-piston pumps, Sections 3.5 .1....
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Introduction to Modern Liquid Chromatography, Third Edition part 17 pdf
... 20 15 0 4.6 5.0 10 ,000 90 b (10 ) c 18 0 (20) 1, 270 (13 0) 15 0 2 .1 5.0 10 ,000 20 (2) 40 (4) 265 (25) 10 0 4.6 3.0 11 ,10 0 60 (6) 11 5 (15 ) 800 (90) 10 0 2 .1 3.0 11 ,10 0 12 (2) 25 (15 ) 17 0 (20) 10 0 2 .1 1.8 ... 1. 8 18 ,500 9 (1) 20 (2) 13 0 (15 ) 10 0 1. 0 1. 8 18 ,500 2 (< ;1) 5 (< ;1) 30 (3) 50 4.6 3.0 5,500 40 (4) 80 (10 ) 565 (60) 50 2 .1 3.0 5,50...
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Introduction to Modern Liquid Chromatography, Third Edition part 19 pdf
... for leaks. REFERENCES 14 5 3. J. N. Brown, M. Hewins, J. H. M. van der Linden, and J. H. M Lynch, J. Chromatogr., 204 (19 81) 11 5. 4. L. R. Snyder, J. Chromatogr. Sci., 21 (19 83) 65. 5. J. Thompson, ... (2000) 17 49. 15 . R. G. Wolcott, J. W. Dolan, L. R. Snyder, S. R. Bakalyar, M. A. Arnold, and J. A. Nichols, J. Chromatogr. A, 869 (2000) 211 . 16 . J. W. Dolan, J. Chromatogr. A, 965...
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Introduction to Modern Liquid Chromatography, Third Edition part 21 pdf
... CHARACTERISTICS 15 9 Table 4.2 Typical Peak Volumes V p0 L (mm) d c (mm) d p (μm) V p0 (μL) a 250 4.6 5 212 15 0 4.6 5 16 4 3 12 7 2 .1 3 26 10 0 4.6 3 10 4 2 .1 3 22 1. 0 3 5 24 50 4.6 3 73 2 .1 3 15 2 .1 2 12 1. 0 ... limited value. 16 0 DETECTION Table 4.4 HPLC Detectors Sample-Specific (Sections 4.4–4 .10 ) Bulk Property (Sections 4 .11 –4 .13 ) Hyphenated (Sections 4 .14 –...
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Introduction to Modern Liquid Chromatography, Third Edition part 31 pdf
... CHROMATOGRAPHY FOR NEUTRAL SAMPLES (b)(a) log k n n = n’ = 8 ii i X X C 18 5 10 15 20 25 1. 40 1. 38 1. 36 α CH 2 n (c) (d ) (e) (f ) 1. 30 1. 28 1. 26 1. 24 5 10 15 20 25 n C 8 α CH 2 1. 28 1. 24 1. 20 C 6 510 152025 n α CH 2 C 1 1. 21 1 .17 1. 13 510 152025 n α CH 2 Figure ... ) (e) (f ) 1. 30 1. 28 1. 26 1. 24 5 10 15 20 25 n C 8 α CH 2 1. 28 1. 24 1. 20 C 6...
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Introduction to Modern Liquid Chromatography, Third Edition part 52 pdf
... York, 19 98, pp. 218 –220. 468 GRADIENT ELUTION 02468 10 Time (min) 20 15 10 25 (a) (b) RPC 0 -10 0% ACN/buffer HILIC 3-40% buffer/ACN 12 NARP #26-29 #7 -17 0246 810 Time (min) 10 5 1 15 20 19 -29 #7 -17 1- 6 Figure ... Waeghe, J. Chromatogr. A, 756 (19 96) 21. 10 . P. Jandera and M. ˇ Spa ˇ cek, J. Chromatogr., 366 (19 86) 10 7. 11 . L. R. Snyder and J. W. Dolan, J. Chrom...
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Introduction to Modern Liquid Chromatography, Third Edition part 65 pdf
... solvents (e.g 1 10 % methanol, propanol, or acetonitrile) can be added to the mobile phase to suppress hydrophobic 13 .4 SEPARATION OF PEPTIDES AND PROTEINS 599 (a) (b) (c) 20 10 0 2468 4 6 8 10 10 2 pI ... exchanger more than (Fig. 13 .15 c). As a guideline, anion-exchange separations are often carried out at 1 to 1. 5 pH units above a protein’s pI, and cation-exchange separat...
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