Introduction to Modern Liquid Chromatography, Third Edition part 95 pdf
Introduction to Modern Liquid Chromatography, Third Edition part 95 pdf
... rules, 583–584structure vs. chromatography, 583–584Proteomics, multidimensional liquid chromatography, 616–618Protocol; see Validation, protocolPseudocritical chromatography, 654Pump(s), 104–113active ... U-HPLCHigh-pressure liquid chromatography, 8High-priced liquid chromatography; seeU-HPLCHigh-speed liquid chromatography, 8HILIC, see Hydrophilic interactionchromatographyHomologs, retention, ... 229–232Hydroxyapatite chromatography, polypeptides, 604–605Hydroxytestosterone isomers, 276Hyphenated detectors; see Detectors,hyphenatedIEC; see Ion-exchange chromatographyImmobilized-metal affinity chromatography, 605–608Indirect...
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Introduction to Modern Liquid Chromatography, Third Edition part 1 pdf
... Hybrid Systems, 111 INTRODUCTION TO MODERN LIQUID CHROMATOGRAPHY Third Edition LLOYD R. SNYDERLC Resources, Inc.Orinda, CAJOSEPH J. KIRKLANDAdvanced Materials TechnologyWilmington, DEJOHN W. ... Lloyd R. Introduction to modern liquid chromatography / Lloyd R. Snyder, Joseph J. Kirkland. – 3rded. / John W. Dolan.p. cm.Includes index.ISBN 978-0-470-16754-0 (cloth)1. Liquid chromatography. ... DOLANLC Resources, Inc.Amity, ORA John Wiley & Sons, Inc., Publication INTRODUCTION TO MODERN LIQUID CHROMATOGRAPHY Third Edition ...
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Introduction to Modern Liquid Chromatography, Third Edition part 4 pdf
... interaction chromatographyHILIC hydrophilic interaction chromatographyi.d. column or tubing inner diameter (mm)IEC ion-exchange chromatographyIPC ion-pair chromatographyk retention factor (same ... retention factor (same as capacity factor k); equal to (tR/t0) − 1k∗gradient retention factor; Equation (9.5)L column length (mm)LC-MS liquid chromatography–mass spectrometryLC-MS/MS ... sales into the first decade of the present century) can be attributed to certain features which continue to be true for the present book. First, all threeeditions have been closely tied to short...
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Introduction to Modern Liquid Chromatography, Third Edition part 16 pdf
... Accumulator-Piston PumpsAn alternative design for the dual-piston pump is the accumulator-piston, ortandem-piston design shown in Figure 3.13d. In this case, one piston feeds intothe other piston ... with either the dual-piston or accumulator-pistondesign.3.5.1.3 Active Check ValveA final refinement in design can be applied to both the dual-piston andaccumulator-piston pump designs. The ball-type ... the top piston (Fig. 3.13d) would pump at 1 mL/min. While the top pistondelivered solvent, the top (outlet) check valve would be open and the intermediatecheck valve (inlet for the top piston)...
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Introduction to Modern Liquid Chromatography, Third Edition part 17 pdf
... cycle time to just a few seconds.Three common autosampler designs are in common use:• pull -to- fill• push -to- fill• needle-in-loop3.6.2.1 Pull -to- Fill AutosamplersThe pull -to- fill autosampler ... detector. One way to avoid this is to use aswitching valve to divert to waste the portion of the column effluent that containsmost of the protein—usually the early portion of the chromatogram ... collector is to use an electroniccircuit to monitor the detector output so that fractions are collected only when apeak is eluted. A delay coil of tubing can be mounted between the detector and...
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Introduction to Modern Liquid Chromatography, Third Edition part 19 pdf
... operator’smanual, replacement of the needle seal every six months will help to avoid problems.The autosampler is the component of the HPLC system that is most likely to leak; itis wise to inspect ... proportioning-manifold replacement, injector rotor,detector lamp, tubing and fittingsFactory-trained Proportioning-manifold repair, electronics, repairs involving modulesubassemblies, detector flow cell3.10.3 RepairsSooner ... DailyaMayvarybasedonfrequencyofuse,performancehistory,orSOP.of the A-reservoir and its contents on a weekly basis should avoid this, althoughsome laboratories stretch this to two weeks. It is a good idea never to ‘‘top off’’ theaqueous...
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Introduction to Modern Liquid Chromatography, Third Edition part 21 pdf
... magneticresonanceRadioactivityConductivityChemiluminescentnitrogenChiral4.3 INTRODUCTION TO INDIVIDUAL DETECTORSThe remainder of this chapter (Sections 4.4–4.16) provides a discussion of mostHPLC detectors in use today. In Table 4.4, detectors are grouped ... selectivity, as well as the ability to change wavelengthsduring a chromatographic run.The most widely used detector in HPLC today is the variable-wavelength UVdetector shown schematically in Figure ... wavelength (or narrow range of wavelengths) of light through theslit and detector cell and onto a photodetector. These detectors usually use a sampleand reference cell configuration (Section 4.4) for...
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Introduction to Modern Liquid Chromatography, Third Edition part 31 pdf
... theinterface between the stationary and mobile phases. Partition (Fig. 6.4c) considersthe stationary phase to be similar to a liquid phase, into which the solute moleculedissolves. Notice that ... back onto or into thestationary phase—rather than extending into the mobile phase as shown.Presumably there is a decreased interaction with the column for solutemolecules that are too long to ... coat the particles, allowing their use for reversed-phase liquid liquid partition chromatography [2], a HPLC technique that is rarely usedtoday.Bonded-phase columns represented the next major...
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Introduction to Modern Liquid Chromatography, Third Edition part 52 pdf
... software that facilitates method development. In this chapter we will Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder,Joseph J. Kirkland, and John W. DolanCopyright ... C. Decrop, J.Chromatogr. A, 1120(2006) 282.76. P. Jandera, J. Chromatogr. A, 965 (2002) 239.77. P. Jandera, J. Chromatogr. A, 1126 (2006) 195. 78. T. Yoshida, J. Chromatogr. A, 60 (2004) 265.79. ... Waeghe, J. Chromatogr., 803 (1998) 1.43. A. P. Schellinger, D. R. Stoll, and P. W. Carr, J. Chromatogr. A, 1064 (2005) 143.44. A. P. Schellinger, D. R. Stoll, and P. W. Carr, J. Chromatogr. A, 1192...
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Introduction to Modern Liquid Chromatography, Third Edition part 65 pdf
... selectivity relative to other modes of col-umn chromatography. Three other chromatographic techniques (chromatofocusing,hydroxyapaptite chromatography, and immobilized-metal affinity chromatography;Sections ... point, binding to an anionexchanger below the protein pI, or binding to a cation exchanger above the pI.Similarly a protein may fail to bind to an anion exchanger above its pI or to a cationexchanger ... columns are in the range of 30 to 120 mg proteinper milliliter of packing.The linker group that joins the ion-exchange group to the support can con-tribute to the chromatographic properties of the...
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