Handbook of Microbiological Media, Fourth Edition part 196 pdf

... temperature. Preparation of YI Broth: Aseptically add 10.0mL of 10X glucose buffer stock to 78.0mL of cooled YI base stock. Adjust osmolarity with NaCl to 380.0milliosmols/kg. Preparation of Medium: Aseptically ... 380.0milliosmols/kg. Preparation of Medium: Aseptically add 2.0mL of vitamin mix- ture 18 and 10.0mL of heat-inactivated bovine serum to 88.0mL of YI broth. Distrib...

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... dilution assay of pen- icillins and other antibiotics. Used in the turbidimetric assay of penicillin and tetracycline with Staphylococcus aureus. For the cultivation and main- tenance of Bacillus ... 11.0g Pancreatic digest of casein 10.0g Yeast extract 6.5g Pancreatic digest of gelatin 5.0g K 2 HPO 4 3.68g NaCl 3.5g Beef extract 1.5g KH 2 PO 4 1.32g pH 6.6 ± 0.2 at 25°C Preparati...

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... Aseptically add 0.05g of chloramphenicol. Mix thoroughly. Pour into sterile Petri dishes. Use: For the isolation and presumptive identification of Cryptococcus neoformans. Cryptococcus neoformans appears ... water and bring volume to 200.0mL. Mix thoroughly. Preparation of Medium: To 400.0mL of solution A, add 400.0mL of solution B and 200.0mL of solution C. Adjust pH to 5.0. Add...

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... inhalation. Preparation of Medium: To 950.0mL of cooled sterile basal medi- um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horse blood, 4.0mL of sterile FBP solution, 8.0mL of sterile cefoperazone ... inhalation. Preparation of Medium: To 950.0mL of cooled sterile basal medi- um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horse blood, 4.0mL o...

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... to 25°C. Add 0.5mL of sterile carbohydrate solution to each tube. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of the Enterobacteriaceae. ... to 25°C. Add 0.5mL of sterile carbohydrate solution to each tube. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of t...

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... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is partially lysed due ... to 50°C. Aseptically add 50.0mL of sterile rabbit blood and 1.5mL of sterile water-lysed blood solution. Water-lysed blood may be omitted if sterile blood is partially lysed due to...

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... 0.2mL of sterile solution A, 0.2mL of sterile solution B, and 0.1mL of sterile solution C for each 10.0mL of medium. Mix thoroughly. Use immediately. Use: For the cultivation and maintenance of ... intensity of 300–500 lux. For heavy cell suspension supplement periodically with sterile yeast extract solution to yield a final concen- tration of 0.1%. Use: For the growth and maint...

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... remove a sufficient amount of medium to permit the addition of feeding medium. Add 1.0mL of feeding solution per each 100.0mL of medium. Use: For the isolation and cultivation of Ectothiorhodospira ... to 25°C. Aseptically add 10.0mL of ster- ile MgCl 2 ·6H 2 O solution, 6.0mL of sterile Na 2 S·9H 2 O solution, 5.0mL of sterile CaCl 2 ·2H 2 O solution, and 4.0mL of sterile N...

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... 1.0g Preparation of Rosolic Acid Solution: Add rosolic acid to 0.2N NaOH and bring volume to 100.0L. Mix thoroughly. Preparation of Medium: Add 10.0mL of rosolic acid solution to 950.0mL of distilled/deionized ... 1.0g Preparation of Rosolic Acid Solution: Add rosolic acid to 0.2N NaOH and bring volume to 100.0L. Mix thoroughly. Preparation of Medium: Add 10.0mL of rosolic aci...

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... rubber stoppers under a gas phase of 80% H 2 + 20% CO 2 (300 kPa). Use: For the cultivation of Persephonella hydrogeniphila and Hydro- genivirga caldilitoris. Hofer’s Alkaline Medium Composition ... separate flask, add potato starch to 50.0mL of distilled/deionized water. Add the starch solution to 450.0mL of boil- ing distilled/deionized water. Add 10.0mL of solution 8 and the ag...

Ngày tải lên: 03/07/2014, 18:20