Handbook of Microbiological Media, Fourth Edition part 86 pdf

Hippea Medium 845 HiCrome™ UTI Agar, Modified (UTI Agar, Modified HiCrome™) Composition per liter: Peptic digest of animal tissue 18.0g Agar 15.0g Chromogenic mixture 12.44g Beef extract 4.0g Casein enzymatic hydrolysate 4.0g pH 7.2 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Mix to completely dissolve components. Do not autoclave. Cool to 50°C. Pour into sterile Petri dishes. Use: A chromogenic medium used for detecting and identifying Enter- obacteria, Proteus species, and other bacteria involved in urinary tract infections. HiFluoro™ Pseudomonas Agar Base Composition per liter: Pancreatic digest of gelatin 18.0g Agar 15.0g K 2 SO 4 10.0g Fluorogenic mixture 2.05g MnCl 2 1.4g Cetrimide 0.3g Glycerol 10.ml pH 7.2 ± 0.2 at 25°C Source: This medium, wihout glycerol, is available as a premixed powder from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes. Use: For the selective isolation of Pseudomonas aeruginosa from clin- ical and nonclinical specimens by the fluorogenic method. High Plate Count Agar Composition per liter: Agar 15.0g Peptic digest of animal tissue 3.0g Casein, soluble 0.5g K 2 HPO 4 0.2g MgSO 4 ·7H 2 O 0.05g FeCl 3 ·4H 2 O 1.0mg pH 7.2 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For obtaining higher colony counts by the spread plate, pour plate or membrane filter technique. High Salt Nutrient Agar Composition per liter: NaCl 30.0g Agar 15.0g Peptic digest of animal tissue 5.0g Meat extract 5.0g pH 8.5 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the isolation and cultivation of salt-tolerant Vibrio species. High Salt Peptone Yeast Extract Agar Composition per liter: NaCl 30.0g Agar 15.0g Peptic digest of animal tissue 10.0g Yeast extract 6.0g Meat extract 2.0g Glucose 2.0g L-Cysteine·HCl·H 2 O 0.3g pH 7.5 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the confirmation of Vibrio species. Hippea Medium (DSMZ Medium 854) Composition per 1010.0mL: NaCl 25.0g Sulfur, powdered 10.0g Na-acetate 5.0g MOPS [3-(N-morpholino) propane sulfonic acid] 3.0g Na 2 S·9H 2 O 0.5g NH 4 Cl 0.33g CaCl 2 ·2H 2 O 0.33g MgCl 2 ·6H 2 O 0.33g KCl 0.33g KH 2 PO 4 0.33g Yeast extract 0.1g Resazurin 0.5mg Trace elements solution 10.0mL Vitamin solution 10.0mL pH 6.1 ± 0.2 at 25°C Trace Elements Solution: Composition per liter: MgSO 4 ·7H 2 O 3.0g Nitrilotriacetic acid 1.5g NaCl 1.0g MnSO 4 ·2H 2 O 0.5g CoSO 4 ·7H 2 O 0.18g ZnSO 4 ·7H 2 O 0.18g © 2010 by Taylor and Francis Group, LLC 846 Hippurate Hydrolysis Broth CaCl 2 ·2H 2 O 0.1g FeSO 4 ·7H 2 O 0.1g NiCl 2 ·6H 2 O 0.025g KAl(SO 4 ) 2 ·12H 2 O 0.02g H 3 BO 3 0.01g Na 2 MoO 4 ·4H 2 O 0.01g CuSO 4 ·5H 2 O 0.01g Na 2 SeO 3 ·5H 2 O 0.3mg Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly. Vitamin Solution: Composition per liter: Pyridoxine-HCl 10.0mg Thiamine-HCl·2H 2 O 5.0mg Riboflavin 5.0mg Nicotinic acid 5.0mg D-Ca-pantothenate 5.0mg p-Aminobenzoic acid 5.0mg Lipoic acid 5.0mg Biotin 2.0mg Folic acid 2.0mg Vitamin B 12 0.1mg Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H 2 + 20% CO 2 . Filter sterilize. Preparation of Medium: Add components, except vitamin solu- tion, sulfur, and Na 2 S·9H 2 O, to distilled/deionized water and bring vol- ume to 1.0L. Mix thoroughly. Adjust pH to 6.0. Sparge the medium with 80% N 2 + 20% CO 2 gas mixture for 30 min. Add Na 2 S·9H 2 O. Mix thoroughly. Readjust the pH to 6.0–6.2. Dispense medium under 80% N 2 + 20% CO 2 gas mixture into anaerobe tubes or bottles con- taining 100.0mg sulfur powder per 10mL medium. Autoclave 20 min at 110°C. Prior to use inject 0.1mL sterile vitamin solution per 10.0mL medium. Use: For the cultivation of Hippea maritima. Hippurate Broth See: Sodium Hippurate Broth Hippurate Hydrolysis Broth Composition per liter: Heart infusion powder 10.0g Peptic digest of animal tissue 10.0g Sodium hippurate 10.0g NaCl 5.0g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the detection of hippurate-hydrolyzing bacteria. Hirschia Medium Composition per liter: Pancreatic digest of casein 5.0g HEPES 4.0g Yeast extract 2.0g Artificial seawater 250.0mL Glucose solution 100.0mL pH 7.4 ± 0.2 at 25°C Glucose Solution: Composition per 100.0mL: Glucose 0.25g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster- ilize. Artificial Seawater: Composition per liter: NaCl 27.5g MgCl 2 ·6H 2 O 5.38g MgSO 4 ·7H 2 O 6.78g KCl 0.72g NaHCO 3 0.2g CaCL 2 ·2H 2 O 1.4g Preparation of Artificial Seawater: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Add components, except glucose solu- tion, to distilled/deionized water and bring volume to 1.0L. Mix thor- oughly. Adjust pH to 7.4. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 25°C. Aseptically add 100.0mL of sterile glucose solu- tion. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Hirschia baltica. Hi-Sensitivity Test Agar Composition per liter: Casein enzymic hydrolysate 11.0g Agar 8.0g NaCl 3.0g Peptic digest of animal tissue 3.0g Glucose 2.0g Na 2 HPO 4 2.0g Sodium acetate 1.0g Starch, soluble 1.0g Magnesium glycerophosphate 0.2g Calcium gluconate 0.1g L-Cystine hydrochloride 0.02g L-Tryptophan 0.02g Adenine 0.01g Guanine 0.01g Uracil 0.01g Xanthine 0.01g Calcium pantothenate 3.0mg Biotin 3.0mg Nicotinamide 3.0mg Pyridoxine hydrochloride 3.0mg Manganese chloride 2.0mg ZnSO 4 1.0mg CoSO 4 1.0mg CuSO 4 1.0mg Cyanocobalamin 1.0mg FeSO 4 1.0mg Menadione 1.0mg Thiamine hydrochloride 0.04mg pH 7.2 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC Hi-Sensitivity Test HiVeg Broth 847 Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes. Use: For antimicrobial susceptibility tests. Hi-Sensitivity Test Broth Composition per liter: Casein enzymic hydrolysate 11.0g NaCl 3.0g Peptic digest of animal tissue 3.0g Glucose 2.0g Na 2 HPO 4 2.0g Sodium acetate 1.0g Starch, soluble 1.0g Magnesium glycerophosphate 0.2g Calcium gluconate 0.1g L-Cystine hydrochloride 0.02g L-Tryptophan 0.02g Adenine 0.01g Guanine 0.01g Uracil 0.01g Xanthine 0.01g Calcium pantothenate 3.0mg Biotin 3.0mg Nicotinamide 3.0mg Pyridoxine hydrochloride 3.0mg Manganese chloride 2.0mg ZnSO 4 1.0mg CoSO 4 1.0mg CuSO 4 1.0mg Cyanocobalamin 1.0mg FeSO 4 1.0mg Menadione 1.0mg Thiamine hydrochloride 0.04mg pH 7.2 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For antimicrobial susceptibility testing. Hi-Sensitivity Test HiVeg Agar Composition per liter: Plant hydrolysate 11.0g Agar 8.0g NaCl 3.0g Plant peptone 3.0g Glucose 2.0g Na 2 HPO 4 2.0g Sodium acetate 1.0g Starch, soluble 1.0g Magnesium glycerophosphate 0.2g Calcium gluconate 0.1g L-Cystine hydrochloride 0.02g L-Tryptophan 0.02g Adenine 0.01g Guanine 0.01g Uracil 0.01g Xanthine 0.01g Calcium pantothenate 3.0mg Biotin 3.0mg Nicotinamide 3.0mg Pyridoxine hydrochloride 3.0mg Manganese chloride 2.0mg ZnSO 4 1.0mg CoSO 4 1.0mg CuSO 4 1.0mg Cyanocobalamin 1.0mg FeSO 4 1.0mg Menadione 1.0mg Thiamine hydrochloride 0.04mg pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes. Use: For antimicrobial susceptibility tests. Hi-Sensitivity Test HiVeg Broth Composition per liter: Plant hydrolysate 11.0g NaCl 3.0g Plant peptone 3.0g Glucose 2.0g Na 2 HPO 4 2.0g Sodium acetate 1.0g Starch, soluble 1.0g Magnesium glycerophosphate 0.2g Calcium gluconate 0.1g L-Cystine hydrochloride 0.02g L-Tryptophan 0.02g Adenine 0.01g Guanine 0.01g Uracil 0.01g Xanthine 0.01g Calcium pantothenate 3.0mg Biotin 3.0mg Nicotinamide 3.0mg Pyridoxine hydrochloride 3.0mg Manganese chloride 2.0mg ZnSO 4 1.0mg CoSO 4 1.0mg CuSO 4 1.0mg Cyanocobalamin 1.0mg FeSO 4 1.0mg Menadione 1.0mg Thiamine hydrochloride 0.04mg pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring © 2010 by Taylor and Francis Group, LLC 848 Hisitest Agar to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For antimicrobial susceptibility testing. Hisitest Agar Composition per liter: Casein enzymic hydrolysate 11.0g Agar 8.0g Buffer salt 3.3g Peptic digest of animal tissue 3.0g NaCl 3.0g Glucose 2.0g Starch 1.0g Nucleoside basis 0.02g Thiamine 0.02mg pH 7.2 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For determination of antibiotic susceptibility of fastidious micro- organisms. Histidans Agar Composition per liter: Agar 20.0g Glucose 10.0g Yeast extract 10.0g Na 2 HPO 4 0.95g KH 2 PO 4 0.91g MgSO 4 ·7H 2 O 0.5g pH 7.0 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of Streptomyces species. Histoplasma capsulatum Agar Composition per liter: Agar 12.5g Glucose 10.0g Citric acid 10.0g Potato starch 2.0g α-Ketoglutaric acid 1.0g L-Cystine·HCl·H 2 O 1.0g Glutathione, reduced 0.5g L-Asparagine 0.1g L-Tryptophan 0.02g Solution 1 250.0mL Solution 3 40.0mL Solution 2 10.0mL Solution 4 10.0mL Solution 8 10.0mL Solution 5 1.0mL Solution 6 0.1mL Solution 7 0.1mL pH 6.5 ± 0.2 at 25°C Solution 1: Composition per liter: KH 2 PO 4 8.0g (NH 4 ) 2 SO 4 8.0g MgSO 4 ·7H 2 O 0.86g CaCl 2 , anhydrous 0.08g ZnSO 4 ·7H 2 O 0.05g Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Bring volume to 1.0L with distilled/deionized water. Store at 5°C. Solution 2: Composition per liter: FeSO 4 ·7H 2 O 5.7g MnCl 2 ·6H 2 O 0.8g NaMoO 4 ·2H 2 O 0.15g HCl, concentrated 1.0mL Preparation of Solution 2: Add 1.0mL of concentrated HCl to 100.0mL of distilled water in a 1.0L volumetric flask. Dissolve each component completely in the sequence given. Bring volume to 1.0L with distilled/deionized water. Store at 5°C. Discard if red color or red precipitate appears. Solution 3: Composition per 100.0mL: Casein, acid-hydrolyzed, vitamin-free 10.0g Preparation of Solution 3: Add casein to distilled/deionized water and bring volume to 100.0mL. Solution 4: Composition per liter: Calcium pantothenate 0.2g Inositol 0.2g Riboflavin 0.2g Thiamine·HCl 0.2g Nicotinamide 0.1g Biotin 0.01g Preparation of Solution 4: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Store at −20°C. Solution 5: Composition per 100.0mL: Hemin 0.2g NH 4 OH, concentrated 0.3mL Preparation of Solution 5: Add hemin to approximately 30.0mL of distilled/deionized water. Add NH 4 OH. Mix thoroughly until dis- solved. Bring volume to 100.0mL with distilled/deionized water. Store at 5° C. Solution 6: Composition per 10.0mL: DL-Thioctic acid 0.01g Ethanol (95% solution) 10.0mL Preparation of Solution 6: Add DL-thioctic acid to 10.0mL of eth- anol. Mix thoroughly. Store at −20°C. Solution 7: Composition per 10.0mL: Coenzyme A 0.01g Na 2 S·5H 2 O (0.05% solution) 0.2mL © 2010 by Taylor and Francis Group, LLC Histoplasma capsulatum Agar 849 Preparation of Solution 7: Prepare Na 2 S·5H 2 O solution in freshly boiled distilled/deionized water. Add coenzyme A to 9.8mL of dis- tilled/deionized water. Mix thoroughly. Add freshly prepared Na 2 S·5H 2 O solution. Mix thoroughly. Store the solution at −20°C. Solution 8: Composition per 100.0mL: Oleic acid 0.1g Preparation of Solution 8: Add oleic acid to 50.0mL of distilled/ deionized water. Adjust pH to 9.0 with NaOH. Gently heat until dis- solved. Bring volume to 100.0mL with distilled/deionized water. Store at 5°C. Preparation of Medium: Add components—except agar, potato starch, and solution 8—to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Adjust pH to 6.5 with 20% KOH solu- tion. Filter sterilize. In a separate flask, add potato starch to 50.0mL of distilled/deionized water. Add the starch solution to 450.0mL of boil- ing distilled/deionized water. Add 10.0mL of solution 8 and the agar. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 70°C. Aseptically combine the two sterile solutions. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Histoplasma capsulatum in the yeast phase. For the cultivation of Histoplasma duboisii, Blasto- myces dermatitidis, and Sprotrichum schenckii. Histoplasma capsulatum Agar Composition per liter: Agar 15.0g Glucose 10.0g Potato starch 2.0g α-Ketoglutaric acid 1.0g L-Cystine·HCl·H 2 O 1.0g Glutathione, reduced 0.5g L-Asparagine 0.1g L-Tryptophan 0.02g Solution 1 250.0mL Solution 3 40.0mL Solution 2 10.0mL Solution 4 10.0mL Solution 8 10.0mL Solution 5 1.0mL Solution 6 0.1mL Solution 7 0.1mL pH 6.5 ± 0.2 at 25°C Solution 1: Composition per liter: KH 2 PO 4 8.0g (NH 4 ) 2 SO 4 8.0g MgSO 4 ·7H 2 O 0.86g CaCl 2 , anhydrous 0.08g Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Bring volume to 1.0L with distilled/deionized water. Store at 5°C. Solution 2: Composition per liter: FeSO 4 ·7H 2 O 5.7g MnCl 2 ·6H 2 O 0.8g NaMoO 4 ·2H 2 O 0.15g HCl, concentrated 1.0mL Preparation of Solution 2: Add the 1.0mL of concentrated HCl to 100.0mL of distilled water in a 1.0L volumetric flask. Dissolve each component completely in the sequence given. Bring volume to 1.0L with distilled/deionized water. Store at 5°C. Discard if red color or red precipitate appears. Solution 3: Composition per 100.0mL: Casein, acid-hydrolyzed, vitamin-free 10.0g Preparation of Solution 3: Add casein to distilled/deionized water and bring volume to 100.0mL. Do not use enzymatically digested ca- sein. Solution 4: Composition per liter: Calcium pantothenate 0.2g Inositol 0.2g Riboflavin 0.2g Thiamine·HCl 0.2g Nicotinamide 0.1g Biotin 0.01g Preparation of Solution 4: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Store at −20°C. Solution 5: Composition per 100.0mL: Hemin 0.2g NH 4 OH, concentrated 0.3mL Preparation of Solution 5: Add hemin to approximately 30.0mL of distilled/deionized water. Add NH 4 OH. Mix thoroughly until dis- solved. Bring volume to 100.0mL with distilled/deionized water. Store at 5°C. Solution 6: Composition per 10.0mL: DL-Thioctic acid 0.01g Ethanol (95% solution) 10.0mL Preparation of Solution 6: Add DL-thioctic acid to 10.0mL of ethanol. Mix thoroughly. Store solution at −20°C. Solution 7: Composition per 10.0mL: Coenzyme A 0.01g Na 2 S·5H 2 O (0.05% solution) 0.2mL Preparation of Solution 7: Prepare Na 2 S·5H 2 O solution in freshly boiled distilled/deionized water. Add coenzyme A to 9.8mL of dis- tilled/deionized water. Mix thoroughly. Add freshly prepared Na 2 S·5H 2 O solution. Mix thoroughly. Store the solution at −20°C. Solution 8: Composition per 100.0mL: Oleic acid 0.1g Preparation of Solution 8: Add oleic acid to 50.0mL of distilled/ deionized water. Adjust pH to 9.0 with NaOH. Gently heat until dis- solved. Bring volume to 100.0mL with distilled/deionized water. Store at 5°C. Preparation of Medium: Add components—except agar, potato starch, and solution 8—to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Adjust pH to 6.5 with 20% KOH solu- tion. Filter sterilize. In a separate flask, add potato starch to 50.0mL of distilled/deionized water. Add the starch solution to 450.0mL of boil- ing distilled/deionized water. Add 10.0mL of solution 8 and the agar. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool © 2010 by Taylor and Francis Group, LLC 850 Histoplasma capsulatum Broth to 70°C. Aseptically combine the two sterile solutions. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Histoplasma capsulatum in the mycelial phase. Histoplasma capsulatum Broth Composition per liter: Glucose 10.0g Citric acid 10.0g α-Ketoglutaric acid 1.0g L-Cystine·HCl·H 2 O 1.0g Potato starch 0.5g Glutathione, reduced 0.5g L-Asparagine 0.1g L-Tryptophan 0.02g Solution 1 250.0mL Solution 3 40.0mL Solution 2 10.0mL Solution 4 10.0mL Solution 5 1.0mL Solution 8 1.0mL Solution 6 0.1mL Solution 7 0.1mL pH 6.5 ± 0.2 at 25°C Solution 1: Composition per liter: KH 2 PO 4 8.0g (NH 4 ) 2 SO 4 8.0g MgSO 4 ·7H 2 O 0.86g CaCl 2 , anhydrous 0.08g ZnSO 4 ·7H 2 O 0.05g Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Bring volume to 1.0L with distilled/deionized water. Store at 5°C. Solution 2: Composition per liter: FeSO 4 ·7H 2 O 5.7g MnCl 2 ·6H 2 O 0.8g NaMoO 4 ·2H 2 O 0.15g HCl, concentrated 1.0mL Preparation of Solution 2: Add 1.0mL of concentrated HCl to 100.0mL of distilled water in a 1.0L volumetric flask. Dissolve each component completely in the sequence given. Bring volume to 1.0L with distilled/deionized water. Store at 5°C. Discard if red color or red precipitate appears. Solution 3: Composition per 100.0mL: Casein, acid-hydrolyzed, vitamin-free 10.0g Preparation of Solution 3: Add casein to distilled/deionized water and bring volume to 100.0mL. Do not use enzymatically digested ca- sein. Solution 4: Composition per liter: Calcium pantothenate 0.2g Inositol 0.2g Riboflavin 0.2g Thiamine·HCl 0.2g Nicotinamide 0.1g Biotin 0.01g Preparation of Solution 4: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Store at −20°C. Solution 5: Composition per 100.0mL: Hemin 0.2g NH 4 OH, concentrated 0.3mL Preparation of Solution 5: Add hemin to approximately 30.0mL of distilled/deionized water. Add NH 4 OH. Mix thoroughly until dis- solved. Bring volume to 100.0mL with distilled/deionized water. Store at 5°C. Solution 6: Composition per 10.0mL: DL-Thioctic acid 0.01g Ethanol (95% solution) 10.0mL Preparation of Solution 6: Add DL-thioctic acid to 10.0mL of eth- anol. Mix thoroughly. Store solution at −20°C. Solution 7: Composition per 10.0mL: Coenzyme A 0.01g Na 2 S·5H 2 O (0.05% solution) 0.2mL Preparation of Solution 7: Prepare Na 2 S·5H 2 O solution in freshly boiled distilled/deionized water. Add coenzyme A to 9.8mL of dis- tilled/deionized water. Mix thoroughly. Add freshly prepared Na 2 S·5H 2 O solution. Mix thoroughly. Store the solution at −20°C. Solution 8: Composition per 100.0mL: Oleic acid 0.1g Preparation of Solution 8: Add oleic acid to 50.0mL of distilled/de- ionized water. Adjust pH to 9.0 with NaOH. Gently heat until dissolved. Bring volume to 100.0mL with distilled/deionized water. Store at 5°C. Preparation of Medium: Add components—except potato starch and solution 8—to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Adjust pH to 6.5 with 20% KOH solution. Filter sterilize. In a separate flask, add potato starch to 50.0mL of dis- tilled/deionized water. Add the starch solution to 450.0mL of boiling distilled/deionized water. Add 1.0mL of solution 8. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 70°C. Asepti- cally combine the two sterile solutions. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Histoplasma capsulatum in the yeast phase. For the cultivation of Histoplasma duboisii, Blastomyces dermatitidis, and Sprotrichum schenckii. HiVeg Hydrolysate Agar with 2.5% Agar Composition per liter: Agar 25.0g Plant hydrolysate 5.0g Plant peptone 5.0g NaCl 5.0g Na 2 HPO 4 2.5g Plant infusion 1.5g Yeast autolysate 1.5g Glycerol 22.0mL pH 7.8 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC HNS Agar 851 Source: This medium, without glycerol, is available as a premixed powder from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Mix thor- oughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Vibrio cholerae. For the production of cholera vaccine. HiVeg Magnesium Broth Composition per liter: Plant hydrolysate 10.0g NaCl 5.0g MgSO 4 0.94g pH 7.0 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Mix thor- oughly. Use: For the cultivation of recombinant strains of Escherichia coli. HiVeg Peptone Water Composition per liter: Plant peptone 10.0g NaCl 5.0g pH 7.2 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Mix thor- oughly. Use: For the cultivation of various bacteria. HL Agar Composition per plate: Columbia agar base 10.0mL Columbia blood top agar 5.0mL pH 7.3 ± 0.2 at 25°C Columbia Agar Base: Composition per liter: Agar 13.5g Pancreatic digest of casein 12.0g NaCl 5.0g Peptic digest of animal tissue 5.0g Beef extract 3.0g Yeast extract 3.0g Cornstarch 1.0g Preparation of Columbia Agar Base: Add components to dis- tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen- tly heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Columbia Blood Top Agar: Composition per liter: Agar 13.5g Pancreatic digest of casein 12.0g NaCl 5.0g Peptic digest of animal tissue 5.0g Beef extract 3.0g Yeast extract 3.0g Cornstarch 1.0g Horse blood, defibrinated 50.0mL Preparation of Columbia Blood Top Agar: Add components, except horse blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add ster- ile horse blood. Mix thoroughly. Preparation of Medium: Pour cooled, sterile Columbia agar base into sterile Petri dishes in 10.0mL volumes. Allow agar to solidify. Pour 5.0mL of cooled, sterile Columbia blood top agar over Columbia agar base that has solidified but is still warm. Use: For the cultivation of Listeria monocytogenes. HM Medium Composition per liter: NaCl 81.0g Yeast extract 10.0g MgSO 4 9.6g MgCl 2 7.0g Proteose peptone No. 3 5.0g KCl 2.0g Glucose 1.0g CaCl 2 0.36g NaHCO 3 60.0mg NaBr 26.0mg pH 7.1 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized wa- ter and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.1. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Salinicoccus roseus and Salinicoccus hispani- cus. HNS Agar (ATCC Medium 923) Composition per liter: Agar 15.0g NaCl 9.6g Heart infusion broth 990.0mL Horse serum 10.0mL pH 7.4 ± 0.2 at 25°C Heart Infusion Broth: Composition per 900.0mL: Beef heart, infusion from 500.0g Tryptose 10.0g NaCl 5.0g Preparation of Heart Infusion Broth: Add agar and NaCl to 990.0mL heart infusion broth. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL sterile horse serum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. © 2010 by Taylor and Francis Group, LLC 852 HNW Medium Use: For the cultivation and maintenance of Corynebacterium species. HNW Medium (DSMZ Medium 997) Composition per liter: DMJ synthetic seawater 1.0L Vitamin solution 10.0mL NaHCO 3 solution 10.0mL NaNO 3 solution 10.0mL Na 2 S·9H 2 O solution 10.0mL Tungstate solution 10.0mL pH 7.2 ± 0.2 at 25°C Tungstate Solution: Composition per 10.0mL: Na 2 WO 4 ·2H 2 O 0.1mg Preparation of Tungstate Solution: Add Na 2 WO 4 ·2H 2 O to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. NaHCO 3 Solution: Composition per 10.0mL: NaHCO 3 1.0g Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de- ionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 20% CO 2 + 80% H 2 . Filter sterilize. NaNO 3 Solution: Composition per 10.0mL: NaNO 3 1.0g Preparation of NaNO 3 Solution: Add NaNO 3 to distilled/deion- ized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N 2 . Filter sterilize. Vitamin Solution: Composition per liter: Pyridoxine-HCl 10.0mg Thiamine-HCl·2H 2 O 5.0mg Riboflavin 5.0mg Nicotinic acid 5.0mg D-Ca-pantothenate 5.0mg p-Aminobenzoic acid 5.0mg Lipoic acid 5.0mg Biotin 2.0mg Folic acid 2.0mg Vitamin B 12 0.1mg Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H 2 + 20% CO 2 . Filter sterilize. Na 2 S·9H 2 O Solution: Composition per 10.0mL: Na 2 S·9H 2 O 0.5g Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N 2 for 15 min at 15 psi pressure–121°C. Cool to room temperature. Adjust pH to 7.5. DMJ Synthetic Seawater: Composition per liter: NaCl 30.0g MgCl 2 ·6H 2 O 4.18g MgSO 4 ·7H 2 O 3.4g KCl 0.33g NH 4 Cl 0.25g K 2 HPO 4 0.14g CaCl 2 ·2H 2 O 0.14g Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O 0.01g NiCl 2 ·6H 2 O 0.5mg Na 2 SeO 3 ·5H 2 O 0.5mg Trace elements solution SL-10 10.0mL Trace Elements Solution SL-10: Composition per liter: MgSO 4 ·7H 2 O 3.0g Nitrilotriacetic acid 1.5g NaCl 1.0g MnSO 4 ·2H 2 O 0.5g CoSO 4 ·7H 2 O 0.18g ZnSO 4 ·7H 2 O 0.18g CaCl 2 ·2H 2 O 0.1g FeSO 4 ·7H 2 O 0.1g NiCl 2 ·6H 2 O 0.025g KAl(SO 4 ) 2 ·12H 2 O 0.02g H 3 BO 3 0.01g Na 2 MoO 4 ·4H 2 O 0.01g CuSO 4 ·5H 2 O 0.01g Na 2 SeO 3 ·5H 2 O 0.3mg Preparation of Trace Elements Solution SL-10: Add nitrilotri- acetic acid to 500.0mL of distilled/deionized water. Dissolve by adjust- ing pH to 6.5 with KOH. Add remaining components. Add distilled/ deionized water to 1.0L. Mix thoroughly. Adjust pH to 7.0. Preparation of DMJ Synthetic Seawater: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper- ature. Preparation of Medium: Aseptically add 10.0mL each of vitamin solution, NaHCO 3 solution, NaNO 3 solution, Na 2 S·9H 2 O solution, and tungstate solution to 1.0L sterile DMJ synthetic seawater. Mix thor- oughly. Distribute into tubes. Tightly seal the tubes with butyl rubber stoppers under a gas phase of 80% H 2 + 20% CO 2 (300 kPa). Use: For the cultivation of Persephonella hydrogeniphila and Hydro- genivirga caldilitoris. Hofer’s Alkaline Medium Composition per liter: Agar 15.0g Mannitol 10.0g Yeast extract 1.0g K 2 HPO 4 0.5g MgSO4 0.2g NaCl 0.1g Thymol Blue 0.016g pH 11.0 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the selective isolation of Agrobacterium spp. from soil. © 2010 by Taylor and Francis Group, LLC Horikoshi-1 Medium with 10% Sodium Chloride 853 Hohn’s Medium, Modified See: Steenken and Smith Agar HO-LE Trace Elements Solution Composition per liter: H 3 BO 3 2.85g MnCl 2 ·4H 2 O 1.8g Sodium tartrate 1.77g FeSO 4 1.36g CoCl 2 ·6H 2 O 0.04g CuCl 2 ·2H 2 O 0.026g Na 2 MoO 4 ·2H 2 O 0.025g ZnCl 2 0.021g Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For use as an enrichment to other media that require trace miner- als. Hominis Agar See: H Agar Hominis Broth See: H Broth Horie Arabinose Ethyl Violet Broth (HAEB) Composition per liter: NaCl 30.0g Peptone 5.0g Beef extract 3.0g Bromthymol Blue 0.03g Ethyl Violet 1.0mg Arabinose solution 100.0mL pH 9.0 ± 0.2 at 25°C Arabinose Solution: Composition per 100.0mL: Arabinose 5.0g Preparation of Arabinose Solution: Add arabinose to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except arabinose solu- tion, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 9.0. Auto- clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti- cally add sterile arabinose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Vibrio species from foods. Horikoshi Alkaline Medium (DSMZ Medium 940) Composition per liter: Agar 15.0g D-glucose 10.0g Peptone 5.0g Yeast extract 5.0g Na 2 CO 3 5.0g KH 2 PO 4 1.0g MgSO 4 ·7H 2 O 0.2g pH 9.0 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Pannonibacter phragmite- tus. Horikoshi-1 Medium (DSMZ Medium 1081) Composition per liter: Agar 15.0g Glucose 10.0g Polypeptone 5.0g Yeast extract 5.0g KH 2 PO 4 1.0g K 2 HPO 4 1.0g MgSO 4 ·7H 2 O 0.2g NaCO 3 solution 100.0mL pH 10.0 ± 0.2 at 25°C NaCO 3 Solution: Composition per 100.0mL: NaCO 3 10.0g Preparation of NaCO 3 Solution: Add NaCO 3 to distilled/deion- ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster- ilize. Preparation of Medium: Add components, except NaCO 3 solu- tion, to double distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 10.0. Gently heat while stirring and bring to boiling. Mix thoroughly. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 50°C. Aseptically add 100.0mL NaCO 3 solution. Ad- just pH to 10.0. Pour into Petri dishes or aseptically distribute into tubes. Use: For the cultivation of “Streptomyces sannurensis” and Salinicoc- cus alkaliphilus. Horikoshi-1 Medium with 10% Sodium Chloride (DSMZ Medium 1081a) Composition per liter: NaCl 100.0g Agar 15.0g Glucose 10.0g Polypeptone 5.0g Yeast extract 5.0g KH 2 PO 4 1.0g K 2 HPO 4 1.0g MgSO 4 ·7H 2 O 0.2g NaCO 3 solution 100.0mL pH 10.0 ± 0.2 at 25°C NaCO 3 Solution: Composition per 100.0mL: NaCO 3 10.0g Preparation of NaCO 3 Solution: Add NaCO 3 to distilled/deion- ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster- ilize. © 2010 by Taylor and Francis Group, LLC 854 Horse Blood Agar Preparation of Medium: Add components, except NaCO 3 solu- tion, to double distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 10.0. Gently heat while stirring and bring to boiling. Mix thoroughly. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 50°C. Aseptically add 100.0mL NaCO 3 solution. Ad- just pH to 10.0. Pour into Petri dishes or aseptically distribute into tubes. Use: For the cultivation of Salinicoccus alkaliphilus. Horse Blood Agar Composition per liter: Beef heart, infusion from 500.0g Agar 15.0g Tryptose 10.0g NaCl 5.0g Horse blood, defibrinated 50.0mL pH 6.8 ± 0.2 at 25°C Preparation of Medium: Add components, except horse blood, to distilled/deionized water and bring volume to 950.0mL. Mix thorough- ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Yersinia pseudotubercu- losis. Horse Serum Agar Composition per liter: Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Horse serum 200.0mL pH 6.8 ± 0.2 at 25°C Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 800.0mL. Mix thorough- ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse se- rum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Pseudomonas aeruginosa and Streptobacillus moniliformis. Horse Serum Broth Composition per liter: Pancreatic digest of gelatin 5.0g Beef extract 3.0g Horse serum 200.0mL pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 800.0mL. Mix thorough- ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse se- rum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Pseudomonas aeruginosa and Streptobacillus moniliformis. Hottinger Broth Composition per liter: Fish peptone 20.0g Yeast extract 2.0g Tryptophan 1.0g pH 7.2 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For cultivation of less fastidious microorganisms and determina- tion of indole as per USSR State Pharmacopoeia. Howardella Medium (DSMZ Medium 1085) Composition per liter: Casitone 20.0g Yeast extract 5.0g Na 2 HPO 4 5.0g MgCl 2 ·6H 2 O 1.1g Urea 1.0g Na-thioglycolate 0.75g Resazurin 0.5mg Urea solution 10.0mL pH 7.4 ± 0.2 at 25°C Urea Solution: Composition per 10.0mL: Urea 1.0g Preparation of Urea Solution: Add urea to distilled/deionized wa- ter and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N 2 . Filter sterilize. Preparation of Medium: Add components, except thioglycolate and urea solution, to double distilled/deionized water and bring volume to 990.0mL. Cool to room temperature while sparging with 80% N 2 + 20% CO 2 . Add thioglycolate. Mix thoroughly. Distribute into tubes or bottles under an atmosphere of 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add 10.0mL urea solution. Adjust pH to 7.4. Use: For the cultivation of Howardella spp. Hoyer’s Medium Composition per liter: (NH 4 ) 2 SO 4 1.0g KH 2 PO 4 0.9g MgSO 4 ·7H 2 O 0.25g K 2 HPO 4 0.1g FeCl 3 ·6H 2 O 0.02g Ethanol solution 200.0mL Ethanol Solution: Composition per 200.0mL: Ethanol 30.0mL Preparation of Ethanol Solution: Add ethanol to distilled/deion- ized water and bring volume to 200.0mL. Mix thoroughly. Filter ster- ilize. © 2010 by Taylor and Francis Group, LLC . 5.7g MnCl 2 ·6H 2 O 0.8g NaMoO 4 ·2H 2 O 0.15g HCl, concentrated 1.0mL Preparation of Solution 2: Add 1.0mL of concentrated HCl to 100.0mL of distilled water in a 1.0L volumetric flask. Dissolve each component. separate flask, add potato starch to 50.0mL of distilled/deionized water. Add the starch solution to 450.0mL of boil- ing distilled/deionized water. Add 10.0mL of solution 8 and the agar. Mix thoroughly 5.7g MnCl 2 ·6H 2 O 0.8g NaMoO 4 ·2H 2 O 0.15g HCl, concentrated 1.0mL Preparation of Solution 2: Add the 1.0mL of concentrated HCl to 100.0mL of distilled water in a 1.0L volumetric flask. Dissolve each component