Handbook of Microbiological Media, Fourth Edition part 121 ppsx

... Filter sterilize. Preparation of Medium: To 954.0mL of sterile cooled base, asep- tically add 20.0mL of sterile dithiothreitol solution, 20.0mL of sterile, defibrinated sheep blood, 5.0mL of sterile Na 2 CO 3 ... gas mix- ture of 80% N 2 + 20% CO 2 . Dispense under the same atmosphere into culture vessels. Fill up to a volume of 20% volume of vessel. Autoclave for 15 min at 1...

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... pressure– 121 C. Aseptically add 0.5mL of sterile L-cysteine solution. Mix thor- oughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of a variety of fungi. Acidaminobacter ... flasks. Autoclave for 10 min at 15 psi pressure 121 C. Use: For the cultivation and maintenance of Acholeplasma species. Acholeplasma Medium (ATCC Medium 1215 ) Composition p...

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... and bring to boiling for 1 min. Autoclave for 15 min at 15 psi pressure 121 C. Cool to 50°–55°C. Add 4.0mL of a 10% solution of L-cysteine·HCl·H 2 O that has been filter sterilized. Mix thoroughly. Pour ... boiling for 1 min. Au- toclave for 15 min at 15 psi pressure 121 C. Cool to 50°–55°C. Add 4.0mL of L-cysteine·HCl·H 2 O solution and 10.0mL of sterile antibiotic solution. Mix tho...

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... 15 psi pressure 121 C. Cool to 50°–55°C. Aseptically add 150.0mL of sterile liver extract, 50.0mL of sterile horse blood, 10.0mL of sterile L- cysteine·HCl solution, 10.0 mL of sterile solution ... 15 psi pressure 121 C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of a variety of microorganisms. For the prep- aration of blood agar by the addition...

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... aseptically add 27.0mL of sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution, 2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so- lution, and 1.0mL of sterile maranil ... min at 15 psi pressure 121 C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster- ile urea solution,...

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... 25.0g Preparation of Glucose Solution: Add 25.0g of glucose to 50.0mL of distilled/deionized water. Mix thoroughly and gently heat to dissolve. Filter sterilize. Preparation of Medium: Add components, ... medium. Aseptically add 72.0mL of sterile rabbit serum. Use: For the cultivation of a wide variety of microorganisms in a chemically defined medium. For the cultivation of Bo...

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... 10.0mL of medium, 0.4mL of sterile Na 2 CO 3 solution, 0.5mL of sterile glucose solution, and 0.5mL of sterile Na 2 S·9H 2 O solution. Af- ter addition of the Na 2 CO 3 solution, the pH of the ... anaerobically add 0.4mL of sterile Na 2 CO 3 solution to each tube containing 10.0mL of medium and 0.5mL of sterile cellobiose solution to each tube. pH of the medium af- ter...

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... Medium: Aseptically combine 980.0mL of sterile solution A with 1.0mL of sterile solution B, 1.0mL of sterile solution C, 10.0mL of sterile solution D, and 10.0mL of sterile solution E, in that order. ... psi pressure 121 C. Aseptically add 167.0mL of sterile artificial seawater and 2.0mL of sterile vitamin solution. Mix thoroughly. Preparation of Medium: Aseptically combine 5...

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... 15 psi pressure 121 C. Aseptically and anaerobically add 50.0mL of sterile NaHCO 3 solution, 20.0mL of sterile KOH solution, 20.0mL of sterile sodium fumarate solution, 20.0mL of ster- ile sodium- L-lactate ... psi pressure 121 C. Aseptically and anaerobically add 0.1mL of sterile Na 2 S·9H 2 O solution, 0.1mL of sterile sodium pyruvate solution, 0.1mL of sterile vitamin soluti...

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... pressure 121 C. Pour into sterile Petri dishes. Use: For the isolation, enumeration, and differentiation of members of Enterobacteriaceae. For the isolation, cultivation, and differentiation of Gram-negative ... to avoid inhalation of the powdered dye and contact with the skin. Preparation of Basic Fuchsin Solution: Add Basic Fuchsin to 10.0mL of ethanol. Mix thoroughly. Preparat...

Ngày tải lên: 03/07/2014, 18:20