Handbook of Microbiological Media, Fourth Edition part 120 ppsx

... cultivation, and characterization of nutritional mutants of Escherichia coli. Also recommended for the isolation and characterization of nutritional mutants from wild-type strains of Bacil- lus subtilis ... Asepti- cally combine 400.0mL of sterile inorganic salt solution, 400.0mL of sterile other component solution, 100.0mL of sterile amino acid solu- tion, and 100.0mL of ster...

Ngày tải lên: 03/07/2014, 18:20

... add 5.0mL of sterile ferric citrate solution and 1.0mL of sterile trace elements solution. Mix thoroughly. Asepti- cally distribute into sterile tubes or flasks. Use: For the cultivation of Rhodopseudomonas ... inoculation, aseptically and anaerobically add 0.25–0.50mL of neutralized sulfide solution. Use: For the cultivation and maintenance of members of the family Rhodospirillac...

Ngày tải lên: 03/07/2014, 18:20

... 100.0mL: FeSO 4 ·7H 2 O 0.2g Preparation of FeSO 4 ·7H 2 O Solution: Dissolve FeSO 4 ·7H 2 O in 100.0mL of distilled/deionized water. Add three drops of concentrated HCl. Mix thoroughly. Vitamin ... isolation of Gram-negative enteric bacilli from clinical and nonclinical specimens. For the isolation and identifi- cation of microorganisms from fecal specimens. For the isolation of...

Ngày tải lên: 03/07/2014, 18:20

... Aseptically add 150.0mL of sterile liver extract, 50.0mL of sterile horse blood, 10.0mL of sterile L- cysteine·HCl solution, 10.0 mL of sterile solution A, and 5.0mL of ster- ile solution B. ... final concentration of 7%. Pour into sterile Petri dishes. Use: For the isolation, cultivation, and detection of hemolytic activity of streptococci, pneumococci, and other particularl...

Ngày tải lên: 03/07/2014, 18:20

... aseptically add 27.0mL of sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution, 2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so- lution, and 1.0mL of sterile maranil ... 45°–50°C. Aseptically add 10.0mL of sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster- ile urea solution, 0.1mL of sterile nystatin solution,...

Ngày tải lên: 03/07/2014, 18:20

... 25.0g Preparation of Glucose Solution: Add 25.0g of glucose to 50.0mL of distilled/deionized water. Mix thoroughly and gently heat to dissolve. Filter sterilize. Preparation of Medium: Add components, ... medium. Aseptically add 72.0mL of sterile rabbit serum. Use: For the cultivation of a wide variety of microorganisms in a chemically defined medium. For the cultivation of Bo...

Ngày tải lên: 03/07/2014, 18:20

... 10.0mL of medium, 0.4mL of sterile Na 2 CO 3 solution, 0.5mL of sterile glucose solution, and 0.5mL of sterile Na 2 S·9H 2 O solution. Af- ter addition of the Na 2 CO 3 solution, the pH of the ... anaerobically add 0.4mL of sterile Na 2 CO 3 solution to each tube containing 10.0mL of medium and 0.5mL of sterile cellobiose solution to each tube. pH of the medium af- ter...

Ngày tải lên: 03/07/2014, 18:20

... Medium: Aseptically combine 980.0mL of sterile solution A with 1.0mL of sterile solution B, 1.0mL of sterile solution C, 10.0mL of sterile solution D, and 10.0mL of sterile solution E, in that order. ... 167.0mL of sterile artificial seawater and 2.0mL of sterile vitamin solution. Mix thoroughly. Preparation of Medium: Aseptically combine 500.0mL of sterile stock extract wi...

Ngày tải lên: 03/07/2014, 18:20

... anaerobically add 50.0mL of sterile NaHCO 3 solution, 20.0mL of sterile KOH solution, 20.0mL of sterile sodium fumarate solution, 20.0mL of ster- ile sodium- L-lactate solution, 10.0mL of sterile Wolfe’s ... Aseptically and anaerobically add 0.1mL of sterile Na 2 S·9H 2 O solution, 0.1mL of sterile sodium pyruvate solution, 0.1mL of sterile vitamin solution, and 0.05mL of s...

Ngày tải lên: 03/07/2014, 18:20

... to avoid inhalation of the powdered dye and contact with the skin. Preparation of Basic Fuchsin Solution: Add Basic Fuchsin to 10.0mL of ethanol. Mix thoroughly. Preparation of Medium: Add components ... 17.7mg Preparation of “Metals 44”: Add sodium EDTA to distilled/de- ionized water and bring volume to 90.0mL. Mix thoroughly. Add a few drops of concentrated H 2 SO 4 to retard...

Ngày tải lên: 03/07/2014, 18:20