Handbook of Microbiological Media, Fourth Edition part 40 doc
Handbook of Microbiological Media, Fourth Edition part 40 doc
... pressure–121°C. Add 0.25mL of
sterile NaHCO
3
solution and 0.25mL of sterile sodium thioglycolate solu-
tion for each 10.0mL of sterile basal medium.
Use: For the cultivation and maintenance of Clostridium ... plate culture of other uri-
nary organisms, while preventing the swarming of Proteus spp. One
chromogen, X-Gluc, is targeted towards β-glucosidase, and allows the
specific...
Handbook of Microbiological Media, Fourth Edition part 204 doc
... Synthetic Minimal Medium, 1939,
1 940
Yeast Tryptone Medium, 1 940
Yeast Tryptone Medium with Streptomycin,
1 940
Yeast Tryptone Starch Medium, 1 940
Yeast Water Agar, 1 940
Yeastolate, 6
Yeast-Peptone-Succinate ... 874, 1 840
Tryptone Peptone Glucose Yeast Extract
I
li Veg Broth Base without Trypsin, 1 840
Tryptone Phosphate Brain Heart Infusion
Yeast Extract Agar. 1 840...
Handbook of Microbiological Media, Fourth Edition part 8 docx
... thoroughly.
Preparation of Bristol's Solution: Add 10.0mL of NaNO
3
solu-
tion, 10.0mL of CaCl
2
solution, 10.0mL of MgSO
4
·7H
2
O solution,
10.0mL of NaNO
3
solution, 10.0mL of K
2
HPO
4
solution, ... Solution:
Composition
per 400 .0mL:
NaNO
3
10.0g
Preparation of NaNO
3
Solution: Add NaNO
3
to distilled/deion-
ized water and bring volume to 400 .0mL. Mix thorou...
Handbook of Microbiological Media, Fourth Edition part 11 doc
... pres-
sure–121°C.
Preparation of Medium: To 916.0mL of sterile solution A add
70.0mL of sterile solution B, 10.0mL of sterile solution C, and 10.0mL
of sterile solution D. Mix thoroughly.
Use: For the cultivation of anaerobic ... yolks from whites. Drain contents of yolk sacs
into sterile stoppered graduate cylinder and discard sacs. Measure
40. 0mL of egg yolk emulsion and...
Handbook of Microbiological Media, Fourth Edition part 13 doc
... to avoid inhalation of the powdered dye and
contact with the skin.
Use: For the determination of carbohydrate fermentation reactions of
microorganisms, particularly members of the Enterobacteriaceae. ... to avoid inhalation of the powdered dye and
contact with the skin.
Use: For the determination of carbohydrate fermentation reactions of
microorganisms, particularly members of...
Handbook of Microbiological Media, Fourth Edition part 15 docx
... determination of phenol coefficients of disinfectant prod-
ucts containing cationic surface-active materials. Use according to
Official Methods of Analysis of the Association of Official Analytical
Chemists ... inoculation of cultures, add 0.25mL of
sterile Na
2
S·9H
2
O solution to each tube containing 9.75mL of sterile basal
medium.
Use: For the cultivation and maintenance...
Handbook of Microbiological Media, Fourth Edition part 18 docx
... and
anaerobically add 50.0mL of sterile cellobiose solution, 10.0mL of
sterile Wolfe’s mineral solutionn, 10.0mL of sterile Wolfe’s vitamin so-
lution, and 10.0mL of sterile Na
2
S·9H
2
O solution. ... cultivation of a variety of bacteria, including Azomonas
species, Azotobacter species, Beijerinckia derxii, Pseudomonas azoto-
colligans, and Rhodococcus erythropolis.
Azotobacter M...
Handbook of Microbiological Media, Fourth Edition part 34 docx
... Aseptically add 50.0mL
of sterile blood and 1.5mL of sterile water-lysed blood solution (one
part blood to three parts water). Water-lysed blood may be omitted if
sterile blood is partially lysed due ... Aseptically add 50.0mL
of sterile blood and 1.5mL of sterile water-lysed blood solution (one
part blood to three parts water). Water-lysed blood may be omitted if
sterile blood is...
Handbook of Microbiological Media, Fourth Edition part 42 docx
... 15.0mL
Preparation of Part B: Prepare a 1M solution of K
2
CO
3
and filter
sterilize. Filter sterilize 25.0mL of 95% ethanol solution. Aseptically
combine 20.0mL of sterile K
2
CO
3
solution and 15.0mL of ... K
2
CO
3
solution and 15.0mL of sterile etha-
nol.
Preparation of Medium: Add 35.0mL of sterile Part B to 965.0mL
of sterile, cooled Part A. Adjust pH to 7.0...
Handbook of Microbiological Media, Fourth Edition part 50 doc
... For the cultivation of Denitrovibrio acetiphilus.
Deoxycholate Agar
Composition per liter:
Agar 16.0g
Lactose 10.0g
NaCl 5.0g
Pancreatic digest of casein 5.0g
Peptic digest of animal tissue 5.0g
K
2
HPO
4
... 100.0mL of sterile glucose solution and 10.0mL of
sterile yeast extract solution. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation...
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