Handbook of Microbiological Media, Fourth Edition part 30 pdf

... Aseptically add 0.05g of chloramphenicol. Mix thoroughly. Pour into sterile Petri dishes. Use: For the isolation and presumptive identification of Cryptococcus neoformans. Cryptococcus neoformans appears ... water and bring volume to 200.0mL. Mix thoroughly. Preparation of Medium: To 400.0mL of solution A, add 400.0mL of solution B and 200.0mL of solution C. Adjust pH to 5.0. Add...

Ngày tải lên: 03/07/2014, 18:20

... dilution assay of pen- icillins and other antibiotics. Used in the turbidimetric assay of penicillin and tetracycline with Staphylococcus aureus. For the cultivation and main- tenance of Bacillus ... 11.0g Pancreatic digest of casein 10.0g Yeast extract 6.5g Pancreatic digest of gelatin 5.0g K 2 HPO 4 3.68g NaCl 3.5g Beef extract 1.5g KH 2 PO 4 1.32g pH 6.6 ± 0.2 at 25°C Preparati...

Ngày tải lên: 03/07/2014, 18:20

... inhalation. Preparation of Medium: To 950.0mL of cooled sterile basal medi- um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horse blood, 4.0mL of sterile FBP solution, 8.0mL of sterile cefoperazone ... inhalation. Preparation of Medium: To 950.0mL of cooled sterile basal medi- um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horse blood, 4.0mL o...

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... to 25°C. Add 0.5mL of sterile carbohydrate solution to each tube. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of the Enterobacteriaceae. ... to 25°C. Add 0.5mL of sterile carbohydrate solution to each tube. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of t...

Ngày tải lên: 03/07/2014, 18:20

... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is partially lysed due ... 500.0mL: Potatoes 300 .0g Preparation of Potato Infusion: Peel and dice potatoes. Add 500.0mL of distilled/deionized water. Gently heat and bring to boiling. Continue boiling for...

Ngày tải lên: 03/07/2014, 18:20

... intensity of 300 –500 lux. For heavy cell suspension supplement periodically with sterile yeast extract solution to yield a final concen- tration of 0.1%. Use: For the growth and maintenance of Chloroflexus ... 0.2mL of sterile solution A, 0.2mL of sterile solution B, and 0.1mL of sterile solution C for each 10.0mL of medium. Mix thoroughly. Use immediately. Use: For the cultiv...

Ngày tải lên: 03/07/2014, 18:20

... remove a sufficient amount of medium to permit the addition of feeding medium. Add 1.0mL of feeding solution per each 100.0mL of medium. Use: For the isolation and cultivation of Ectothiorhodospira ... Gently heat at 100°C for 30 min. Do not auto- clave. Cool rapidly to 25°C. Use: For the cultivation and enrichment of members of the Enterobac- teriaceae in the examination of...

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... 1.0g Preparation of Rosolic Acid Solution: Add rosolic acid to 0.2N NaOH and bring volume to 100.0L. Mix thoroughly. Preparation of Medium: Add 10.0mL of rosolic acid solution to 950.0mL of distilled/deionized ... for 30 min. Rinse with cold water twice. Add 1.0L of distilled/deionized water, boil 30 min, and rinse. Repeat this process at least five times. Dry the extract- ed h...

Ngày tải lên: 03/07/2014, 18:20

... rubber stoppers under a gas phase of 80% H 2 + 20% CO 2 (300 kPa). Use: For the cultivation of Persephonella hydrogeniphila and Hydro- genivirga caldilitoris. Hofer’s Alkaline Medium Composition ... separate flask, add potato starch to 50.0mL of distilled/deionized water. Add the starch solution to 450.0mL of boil- ing distilled/deionized water. Add 10.0mL of solution 8 and the ag...

Ngày tải lên: 03/07/2014, 18:20

... 4.0mL: FeCl 3 ·6H 2 O 30. 0mg Preparation of Ferric Chloride Solution: Add FeCl 3 ·6H 2 O to 4.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, ... Aseptically combine 500.0mL of solu- tion A and 500.0mL of solution B. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation...

Ngày tải lên: 03/07/2014, 18:20