... of cooled, sterile base broth, aseptically add 10.0mL of sterile yeast di-alysate, 20.0mL of horse serum, 2.0mL of sterile penicillin solution,1.0mL of sterile glucose solution, and 1.0mL of ... toxic.Preparation of Medium: To 65.0mL of cooled, sterile base agar,aseptically add 10.0mL of sterile yeast dialysate, 20.0mL of horse se-rum, 2.0mL of sterile penicillin solution, and 1.0mL of sterile ... add 20.0mL of sterile substrate solution,10.0mL of sterile MgSO4·7H2O solution, 10.0mL of sterileCaCl2·2H2O solution, 1.0mL of sterile selenite-tungstate solution,10.0mL of sterile...