Handbook of Microbiological Media, Fourth Edition part 21 ppsx
Handbook of Microbiological Media, Fourth Edition part 21 ppsx
... bring to boiling for 1 min. Autoclave for 15 min at 15 psi
pressure– 121 C. Cool to 50°–55°C. Add 4.0mL of a 10% solution of
L-cysteine·HCl·H
2
O that has been filter sterilized. Mix thoroughly.
Pour ... boiling for 1 min. Au-
toclave for 15 min at 15 psi pressure– 121 C. Cool to 50°–55°C. Add
4.0mL of
L-cysteine·HCl·H
2
O solution and 10.0mL of sterile antibiotic
solution. Mix thoro...
Handbook of Microbiological Media, Fourth Edition part 6 ppsx
... pressure–
121 C. Aseptically add 0.5mL of sterile
L-cysteine solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of a variety of fungi.
Acidaminobacter ... flasks. Autoclave for 10 min at
15 psi pressure– 121 C.
Use: For the cultivation and maintenance of Acholeplasma species.
Acholeplasma Medium
(ATCC Medium 1215 )
Composition p...
Handbook of Microbiological Media, Fourth Edition part 24 ppsx
... 15 psi
pressure– 121 C. Cool to 50°–55°C. Aseptically add 150.0mL of sterile
liver extract, 50.0mL of sterile horse blood, 10.0mL of sterile
L-
cysteine·HCl solution, 10.0 mL of sterile solution ... 15
psi pressure– 121 C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of a variety of microorganisms. For the prep-
aration of blood agar by the additio...
Handbook of Microbiological Media, Fourth Edition part 28 ppsx
... pressure– 121 C. Cool to 45°–50°C. Aseptically add 10.0mL of
sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster-
ile urea solution, 0.1mL of sterile nystatin solution, and 0.1mL of ... aseptically add 27.0mL of
sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution,
2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so...
Handbook of Microbiological Media, Fourth Edition part 29 ppsx
... 25.0g
Preparation of Glucose Solution: Add 25.0g of glucose to 50.0mL
of distilled/deionized water. Mix thoroughly and gently heat to dissolve.
Filter sterilize.
Preparation of Medium: Add components, ... medium. Aseptically add 72.0mL of sterile rabbit
serum.
Use: For the cultivation of a wide variety of microorganisms in a
chemically defined medium. For the cultivation of Bo...
Handbook of Microbiological Media, Fourth Edition part 44 ppsx
... 10.0mL of medium, 0.4mL of sterile Na
2
CO
3
solution, 0.5mL of
sterile glucose solution, and 0.5mL of sterile Na
2
S·9H
2
O solution. Af-
ter addition of the Na
2
CO
3
solution, the pH of the ... anaerobically add 0.4mL of sterile
Na
2
CO
3
solution to each tube containing 10.0mL of medium and
0.5mL of sterile cellobiose solution to each tube. pH of the medium af-
ter...
Handbook of Microbiological Media, Fourth Edition part 47 ppsx
... Medium: Aseptically combine 980.0mL of sterile
solution A with 1.0mL of sterile solution B, 1.0mL of sterile solution
C, 10.0mL of sterile solution D, and 10.0mL of sterile solution E, in
that order. ... psi pressure– 121 C.
Aseptically add 167.0mL of sterile artificial seawater and 2.0mL of
sterile vitamin solution. Mix thoroughly.
Preparation of Medium: Aseptically combine...
Handbook of Microbiological Media, Fourth Edition part 51 ppsx
... 15 psi pressure– 121 C. Aseptically
and anaerobically add 50.0mL of sterile NaHCO
3
solution, 20.0mL of sterile
KOH solution, 20.0mL of sterile sodium fumarate solution, 20.0mL of ster-
ile sodium-
L-lactate ... pressure– 121 C. Aseptically and anaerobically add 0.1mL of
sterile Na
2
S·9H
2
O solution, 0.1mL of sterile sodium pyruvate solution,
0.1mL of sterile vitamin solution...
Handbook of Microbiological Media, Fourth Edition part 65 ppsx
... pressure– 121 C. Pour into sterile Petri dishes.
Use: For the isolation, enumeration, and differentiation of members of
Enterobacteriaceae. For the isolation, cultivation, and differentiation of
Gram-negative ... to avoid inhalation of the powdered dye and contact with the
skin.
Preparation of Basic Fuchsin Solution: Add Basic Fuchsin to
10.0mL of ethanol. Mix thoroughly.
Prepara...
Handbook of Microbiological Media, Fourth Edition part 78 ppsx
... of
cooled, sterile base broth, aseptically add 10.0mL of sterile yeast di-
alysate, 20.0mL of horse serum, 2.0mL of sterile penicillin solution,
1.0mL of sterile glucose solution, and 1.0mL of ... toxic.
Preparation of Medium: To 65.0mL of cooled, sterile base agar,
aseptically add 10.0mL of sterile yeast dialysate, 20.0mL of horse se-
rum, 2.0mL of sterile penicillin so...
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