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Handbook of Microbiological Media, Fourth Edition part 14 pdf

Handbook of Microbiological Media, Fourth Edition part 14 pdf

Handbook of Microbiological Media, Fourth Edition part 14 pdf

... dilution assay of pen-icillins and other antibiotics. Used in the turbidimetric assay of penicillinand tetracycline with Staphylococcus aureus. For the cultivation and main-tenance of Bacillus ... 11.0gPancreatic digest of casein 10.0gYeast extract 6.5gPancreatic digest of gelatin 5.0gK2HPO4 3.68gNaCl 3.5gBeef extract 1.5gKH2PO4 1.32gpH 6.6 ± 0.2 at 25°CPreparation of Medium: Add ... assay of carbenicillin, colistimethate, and polymyxin B.Antibiotic Medium 11(Neomycin Assay Agar)Composition per liter:Agar 15.0gPancreatic digest of gelatin 6.0gPancreatic digest of casein...
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Handbook of Microbiological Media, Fourth Edition part 30 pdf

Handbook of Microbiological Media, Fourth Edition part 30 pdf

... Aseptically add 0.05g of chloramphenicol.Mix thoroughly. Pour into sterile Petri dishes.Use: For the isolation and presumptive identification of Cryptococcusneoformans. Cryptococcus neoformans appears ... waterand bring volume to 200.0mL. Mix thoroughly. Preparation of Medium: To 400.0mL of solution A, add 400.0mL of solution B and 200.0mL of solution C. Adjust pH to 5.0. Add agar.Mix thoroughly. ... 20.0mL of sterile car-bohydrate solution, 10.0mL of sterile L-cysteine·HCl·H2O solution,and 10.0mL of sterile Na2S·9H2O solution or, using a syringe, injectthe appropriate amount of sterile...
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Handbook of Microbiological Media, Fourth Edition part 31 pdf

Handbook of Microbiological Media, Fourth Edition part 31 pdf

... inhalation.Preparation of Medium: To 950.0mL of cooled sterile basal medi-um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horseblood, 4.0mL of sterile FBP solution, 8.0mL of sterile cefoperazone ... inhalation.Preparation of Medium: To 950.0mL of cooled sterile basal medi-um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horseblood, 4.0mL of sterile FBP solution, 4.0mL of sterile cefoperazone ... 4.0mL of sterile cefoperazone solution, 4.0mL of sterile trimethoprim lactate solution, 4.0mL of sterile vancomycinsolution, and either 4.0mL of sterile cycloheximide solution or 4.0mL of sterile...
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Handbook of Microbiological Media, Fourth Edition part 32 pdf

Handbook of Microbiological Media, Fourth Edition part 32 pdf

... to 25°C. Add 0.5mL of sterilecarbohydrate solution to each tube.Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of the Enterobacteriaceae. ... to 25°C. Add 0.5mL of sterilecarbohydrate solution to each tube.Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of the Enterobacteriaceae. ... to 25°C. Add 0.5mL of sterilecarbohydrate solution to each tube.Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of the Enterobacteriaceae....
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Handbook of Microbiological Media, Fourth Edition part 33 pdf

Handbook of Microbiological Media, Fourth Edition part 33 pdf

... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted ifsterile blood is partially lysed due ... to 50°C. Aseptically add50.0mL of sterile rabbit blood and 1.5mL of sterile water-lysed bloodsolution. Water-lysed blood may be omitted if sterile blood is partiallylysed due to storage. Mix ... Aseptically add 1.0mL of sterile trace salts solution and 5.0mL of sterile lincomycin solution.Mix thoroughly. Aseptically distribute into sterile tubes or flasks.Use: For the cultivation of heat-labile,...
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Handbook of Microbiological Media, Fourth Edition part 37 pdf

Handbook of Microbiological Media, Fourth Edition part 37 pdf

... 0.2mL of sterile solution A, 0.2mL of sterile solutionB, and 0.1mL of sterile solution C for each 10.0mL of medium. Mixthoroughly. Use immediately.Use: For the cultivation and maintenance of ... intensity of 300–500 lux. For heavy cell suspension supplementperiodically with sterile yeast extract solution to yield a final concen-tration of 0.1%.Use: For the growth and maintenance of Chloroflexus ... 2500UPreparation of Supplement B: Add components to 10.0mL of dis-tilled/deionized water. Mix thoroughly. Filter sterilize.Source: Supplement B is available from BD Diagnostic Systems.Preparation of Medium:...
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Handbook of Microbiological Media, Fourth Edition part 64 pdf

Handbook of Microbiological Media, Fourth Edition part 64 pdf

... remove a sufficient amount of medium to permit the addition of feeding medium. Add 1.0mL of feeding solution per each 100.0mL of medium.Use: For the isolation and cultivation of Ectothiorhodospira ... to 25°C. Aseptically add 10.0mL of ster-ile MgCl2·6H2O solution, 6.0mL of sterile Na2S·9H2O solution, 5.0mL of sterile CaCl2·2H2O solution, and 4.0mL of sterile NH4Cl solution.Mix ... to 25°C. Aseptically add 10.0mL of ster-ile MgCl2·6H2O solution, 6.0mL of sterile Na2S·9H2O solution, 5.0mL of sterile CaCl2·2H2O solution, and 4.0mL of sterile NH4Cl solution.Mix...
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Handbook of Microbiological Media, Fourth Edition part 68 pdf

Handbook of Microbiological Media, Fourth Edition part 68 pdf

... 1.0gPreparation of Rosolic Acid Solution: Add rosolic acid to 0.2NNaOH and bring volume to 100.0L. Mix thoroughly. Preparation of Medium: Add 10.0mL of rosolic acid solution to950.0mL of distilled/deionized ... 1.0gPreparation of Rosolic Acid Solution: Add rosolic acid to 0.2NNaOH and bring volume to 100.0L. Mix thoroughly. Preparation of Medium: Add 10.0mL of rosolic acid solution to950.0mL of distilled/deionized ... 99.0mLPreparation of Vitamin K1 Solution: Add vitamin K1 to 99.0mL of absolute ethanol. Mix thoroughly. Hemin Solution:Composition per 100.0mL:Hemin 1.0gNaOH (1N solution) 20.0mLPreparation of Hemin...
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Handbook of Microbiological Media, Fourth Edition part 86 pdf

Handbook of Microbiological Media, Fourth Edition part 86 pdf

... rubberstoppers under a gas phase of 80% H2 + 20% CO2 (300 kPa).Use: For the cultivation of Persephonella hydrogeniphila and Hydro-genivirga caldilitoris.Hofer’s Alkaline MediumComposition ... separate flask, add potato starch to 50.0mL of distilled/deionized water. Add the starch solution to 450.0mL of boil-ing distilled/deionized water. Add 10.0mL of solution 8 and the agar.Mix thoroughly. ... 5.7gMnCl2·6H2O 0.8gNaMoO4·2H2O 0.15gHCl, concentrated 1.0mLPreparation of Solution 2: Add the 1.0mL of concentrated HCl to100.0mL of distilled water in a 1.0L volumetric flask. Dissolve eachcomponent...
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Handbook of Microbiological Media, Fourth Edition part 105 pdf

Handbook of Microbiological Media, Fourth Edition part 105 pdf

... 100.0mL of sterile phenol solu-tion and 1.0mL of sterile trace elements solution SL-4. Mix thoroughly.Aseptically distribute into sterile tubes or flasks.Use: For the cultivation of Bacillus ... Aseptically combine 500.0mL of solu-tion A and 500.0mL of solution B. Mix thoroughly. Pour into sterilePetri dishes or distribute into sterile tubes.Use: For the cultivation of aciduric, thermophilic ... into sterile tubes. Use: Recommended by the Directive of the Council of the EuropeanUnion 98/83/EC for the isolation and enumeration of Clostridium per-fringens from water samples using the...
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