Handbook of Microbiological Media, Fourth Edition part 11 doc

... pres- sure–121°C. Preparation of Medium: To 916.0mL of sterile solution A add 70.0mL of sterile solution B, 10.0mL of sterile solution C, and 10.0mL of sterile solution D. Mix thoroughly. Use: For the cultivation of anaerobic ... 70.0mL Preparation of Egg Yolk Emulsion: Soak eggs with 1:100 dilu- tion of saturated mercuric chloride solution for 1 min. Crack 11 eggs and sepa...

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... 709, 732,857, 932, 933,991.992,993.1005.1026. 1031. 1032, 1053, 1066, 1067, 1069, 115 6, 115 7 .116 7 .116 8 ,118 6 .119 6. 1227. 1228,1242, 1243, 1244, 1245, 1246, 1252,1253, 1256,1303, 1314, 1318, ... Wallerstein Laboratory Differential Medium. 1910,1 911 Wallerstein Laboratory Medium with Toma- to Juice, 1 911 Wallcrstcin Laboratory Nutrient Agar. 1 911 Wallerstein Laborato...

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... 10.0mL of CaCl 2 solution, 10.0mL of MgSO 4 ·7H 2 O solution, 10.0mL of NaNO 3 solution, 10.0mL of K 2 HPO 4 solution, 10.0mL of KH 2 PO 4 solution, and 10.0mL of NaCl solution to distilled/deionized water ... 1.0g Preparation of FeCl 3 Solution: Add FeCl 3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Preparation of Bristol's Solution: A...

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... to avoid inhalation of the powdered dye and contact with the skin. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of the Enterobacteriaceae. ... to avoid inhalation of the powdered dye and contact with the skin. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of...

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... determination of phenol coefficients of disinfectant prod- ucts containing cationic surface-active materials. Use according to Official Methods of Analysis of the Association of Official Analytical Chemists ... inoculation of cultures, add 0.25mL of sterile Na 2 S·9H 2 O solution to each tube containing 9.75mL of sterile basal medium. Use: For the cultivation and maintenance...

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... and anaerobically add 50.0mL of sterile cellobiose solution, 10.0mL of sterile Wolfe’s mineral solutionn, 10.0mL of sterile Wolfe’s vitamin so- lution, and 10.0mL of sterile Na 2 S·9H 2 O solution. ... pressure–121°C. Aseptically and anaerobically add 10.0mL of sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s vitamin so- lution, and 10.0mL of sterile Na 2 S·9H 2 O sol...

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... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is partially lysed due ... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is...

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... pressure–121°C. Add 0.25mL of sterile NaHCO 3 solution and 0.25mL of sterile sodium thioglycolate solu- tion for each 10.0mL of sterile basal medium. Use: For the cultivation and maintenance of Clostridium ... plate culture of other uri- nary organisms, while preventing the swarming of Proteus spp. One chromogen, X-Gluc, is targeted towards β-glucosidase, and allows the specific...

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... 15.0mL Preparation of Part B: Prepare a 1M solution of K 2 CO 3 and filter sterilize. Filter sterilize 25.0mL of 95% ethanol solution. Aseptically combine 20.0mL of sterile K 2 CO 3 solution and 15.0mL of ... K 2 CO 3 solution and 15.0mL of sterile etha- nol. Preparation of Medium: Add 35.0mL of sterile Part B to 965.0mL of sterile, cooled Part A. Adjust pH to 7.0...

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... For the cultivation of Denitrovibrio acetiphilus. Deoxycholate Agar Composition per liter: Agar 16.0g Lactose 10.0g NaCl 5.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g K 2 HPO 4 ... 100.0mL of sterile glucose solution and 10.0mL of sterile yeast extract solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation...

Ngày tải lên: 03/07/2014, 18:20