Handbook of Microbiological Media, Fourth Edition part 6 ppsx

... 1.5g CoCl 2 ·6H 2 O 190.0mg MnCl 2 ·4H 2 O 100.0mg ZnCl 2 70.0mg Na 2 MoO 4 ·2H 2 O 36. 0mg NiCl 2 ·6H 2 O 24.0mg H 3 BO 3 6. 0mg CuCl 2 ·2H 2 O 2.0mg HCl (25% solution) 10.0mL Preparation of Trace ... 70.0mg Na 2 MoO 4 ·2H 2 O 36. 0mg NiCl 2 ·6H 2 O 24.0mg H 3 BO 3 6. 0mg CuCl 2 ·2H 2 O 2.0mg HCl (25% solution) 10.0mL Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O...

Ngày tải lên: 03/07/2014, 18:20

... Solution: Composition per liter: FeCl 3 ·6H 2 O 0.6g MnCl 2 ·4H 2 O 0.6g ZnCl 2 0.6g CuSO 4 ·5H 2 O 0.6g CaCl 2 ·2H 2 O 0.6g NaCl 0.6g Preparation of Salt Solution: Add components to 1.0L of dis- tilled/deionized ... liter: CaCl 2 ·2H 2 O 0 .66 g Na 2 MoO 4 ·2H 2 O 0.3g ZnSO 4 ·7H 2 O 0.18g CoCl 2 ·6H 2 O 0.18g CuSO 4 ·5H 2 O 0.16g MnSO 4 ·4H 2 O 0.15g H 3 BO 3 0.1g Preparation of Tr...

Ngày tải lên: 03/07/2014, 18:20

... 170.0mL: Liver powder 10.0g Preparation of Liver Extract: Add 10.0g of liver powder to 170mL of distilled/deionized water. Gently heat to 60 °C. Maintain at 50° 60 °C for 1 hr. Gently bring to boiling. ... Aseptically add 150.0mL of sterile liver extract, 50.0mL of sterile horse blood, 10.0mL of sterile L- cysteine·HCl solution, 10.0 mL of sterile solution A, and 5.0mL of st...

Ngày tải lên: 03/07/2014, 18:20

... aseptically add 27.0mL of sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution, 2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so- lution, and 1.0mL of sterile maranil ... 45°–50°C. Aseptically add 10.0mL of sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster- ile urea solution, 0.1mL of sterile nystatin solution,...

Ngày tải lên: 03/07/2014, 18:20

... 1 066 , without glutamine, without bicarbonate, 10X 100.0mL Rabbit serum 64 .0mL pH 7 .6 7 .65 at 25°C CMRL 1 066 Medium without Glutamine, without Bicar- bonate, 10X: Composition per liter: NaCl 6. 8g D-Glucose ... Aseptically add 100.0mL of sterile CMRL 1 066 and 64 .0mL of sterile rabbit serum. Adjust final pH to 7.5–7 .6. Aseptically distribute into sterile tubes or flasks. Use:...

Ngày tải lên: 03/07/2014, 18:20

... pressure–121°C. Use: For the cultivation and maintenance of Fusobacterium varium. © 2010 by Taylor and Francis Group, LLC 432 CMRL-1 066 Medium with Glutamine, 10X CMRL-1 066 Medium with Glutamine, 10X (Connaught ... 70.0mg Na 2 MoO 4 ·2H 2 O 36. 0mg NiCl 2 ·6H 2 O 24.0mg H 3 BO 3 6. 0mg CuCl 2 ·2H 2 O 2.0mg HCl (25% solution) 10.0mL Preparation of Trace Elements Solution SL-10: Add FeC...

Ngày tải lên: 03/07/2014, 18:20

... add 167 .0mL of sterile artificial seawater and 2.0mL of sterile vitamin solution. Mix thoroughly. Preparation of Medium: Aseptically combine 500.0mL of sterile stock extract with 500.0mL of sterile ... 100% N 2 . Preparation of Medium: Aseptically and anoxically combine 65 0.0mL of solution A with 350.0mL of Clostridum sporogenes super- natant. Use: For the cultivation of...

Ngày tải lên: 03/07/2014, 18:20

... anaerobically add 50.0mL of sterile NaHCO 3 solution, 20.0mL of sterile KOH solution, 20.0mL of sterile sodium fumarate solution, 20.0mL of ster- ile sodium- L-lactate solution, 10.0mL of sterile Wolfe’s ... 1.5g CoCl 2 ·6H 2 O 190.0mg MnCl 2 ·4H 2 O 100.0mg ZnCl 2 70.0mg Na 2 MoO 4 ·2H 2 O 36. 0mg NiCl 2 ·6H 2 O 24.0mg H 3 BO 3 6. 0mg CuCl 2 ·2H 2 O 2.0mg HCl (25% solution) 10....

Ngày tải lên: 03/07/2014, 18:20

... liter: NaNO 3 4 .66 7g Na 2 SiO 3 ·9H 2 O 3.0g Sodium glycerophosphate 0 .66 7g EDTA·2H 2 O 0.553g H 3 BO 3 0.38g Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O 0.234g MnSO 4 ·4H 2 O 0.054g FeCl 3 ·6H 2 O 0.016g ZnSO 4 ·7H 2 O ... pH to 6. 8. Artificial Sea Water: Composition per liter: NaCl 23.477g MgCl 2 ·6H 2 O 4.981g Na 2 SO 4 3.917g CaCl 2 1.12g KCl 66 4.0mg NaHCO 3 192.0mg H 3 BO 3 26. 0mg SrCl...

Ngày tải lên: 03/07/2014, 18:20

... toxic. Preparation of Medium: To 65 .0mL of cooled, sterile base agar, aseptically add 10.0mL of sterile yeast dialysate, 20.0mL of horse se- rum, 2.0mL of sterile penicillin solution, and 1.0mL of sterile ... pressure–121°C. Allow to stand. Remove supernatant so- lution. Adjust pH to 6. 6 6. 8. Filter sterilize. Preparation of Medium: To 67 5.0mL of cooled, sterile Columbi...

Ngày tải lên: 03/07/2014, 18:20