Handbook of Microbiological Media, Fourth Edition part 6 ppsx
Handbook of Microbiological Media, Fourth Edition part 6 ppsx
... 1.5g
CoCl
2
·6H
2
O 190.0mg
MnCl
2
·4H
2
O 100.0mg
ZnCl
2
70.0mg
Na
2
MoO
4
·2H
2
O 36. 0mg
NiCl
2
·6H
2
O 24.0mg
H
3
BO
3
6. 0mg
CuCl
2
·2H
2
O 2.0mg
HCl (25% solution) 10.0mL
Preparation of Trace ... 70.0mg
Na
2
MoO
4
·2H
2
O 36. 0mg
NiCl
2
·6H
2
O 24.0mg
H
3
BO
3
6. 0mg
CuCl
2
·2H
2
O 2.0mg
HCl (25% solution) 10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl
2
·4H
2
O...
Handbook of Microbiological Media, Fourth Edition part 21 ppsx
... Solution:
Composition
per liter:
FeCl
3
·6H
2
O 0.6g
MnCl
2
·4H
2
O 0.6g
ZnCl
2
0.6g
CuSO
4
·5H
2
O 0.6g
CaCl
2
·2H
2
O 0.6g
NaCl 0.6g
Preparation of Salt Solution: Add components to 1.0L of dis-
tilled/deionized ... liter:
CaCl
2
·2H
2
O 0 .66 g
Na
2
MoO
4
·2H
2
O 0.3g
ZnSO
4
·7H
2
O 0.18g
CoCl
2
·6H
2
O 0.18g
CuSO
4
·5H
2
O 0.16g
MnSO
4
·4H
2
O 0.15g
H
3
BO
3
0.1g
Preparation of Tr...
Handbook of Microbiological Media, Fourth Edition part 24 ppsx
... 170.0mL:
Liver powder 10.0g
Preparation of Liver Extract: Add 10.0g of liver powder to
170mL of distilled/deionized water. Gently heat to 60 °C. Maintain at
50° 60 °C for 1 hr. Gently bring to boiling. ... Aseptically add 150.0mL of sterile
liver extract, 50.0mL of sterile horse blood, 10.0mL of sterile
L-
cysteine·HCl solution, 10.0 mL of sterile solution A, and 5.0mL of st...
Handbook of Microbiological Media, Fourth Edition part 28 ppsx
... aseptically add 27.0mL of
sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution,
2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so-
lution, and 1.0mL of sterile maranil ... 45°–50°C. Aseptically add 10.0mL of
sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster-
ile urea solution, 0.1mL of sterile nystatin solution,...
Handbook of Microbiological Media, Fourth Edition part 29 ppsx
... 1 066 , without glutamine,
without bicarbonate, 10X 100.0mL
Rabbit serum 64 .0mL
pH 7 .6 7 .65 at 25°C
CMRL 1 066 Medium without Glutamine, without Bicar-
bonate, 10X:
Composition
per liter:
NaCl 6. 8g
D-Glucose ... Aseptically add
100.0mL of sterile CMRL 1 066 and 64 .0mL of sterile rabbit serum.
Adjust final pH to 7.5–7 .6. Aseptically distribute into sterile tubes or
flasks.
Use:...
Handbook of Microbiological Media, Fourth Edition part 44 ppsx
... pressure–121°C.
Use: For the cultivation and maintenance of Fusobacterium varium.
© 2010 by Taylor and Francis Group, LLC
432 CMRL-1 066 Medium with Glutamine, 10X
CMRL-1 066 Medium with Glutamine, 10X
(Connaught ... 70.0mg
Na
2
MoO
4
·2H
2
O 36. 0mg
NiCl
2
·6H
2
O 24.0mg
H
3
BO
3
6. 0mg
CuCl
2
·2H
2
O 2.0mg
HCl (25% solution) 10.0mL
Preparation of Trace Elements Solution SL-10: Add FeC...
Handbook of Microbiological Media, Fourth Edition part 47 ppsx
... add 167 .0mL of sterile artificial seawater and 2.0mL of
sterile vitamin solution. Mix thoroughly.
Preparation of Medium: Aseptically combine 500.0mL of sterile
stock extract with 500.0mL of sterile ... 100%
N
2
.
Preparation of Medium: Aseptically and anoxically combine
65 0.0mL of solution A with 350.0mL of Clostridum sporogenes super-
natant.
Use: For the cultivation of...
Handbook of Microbiological Media, Fourth Edition part 51 ppsx
... anaerobically add 50.0mL of sterile NaHCO
3
solution, 20.0mL of sterile
KOH solution, 20.0mL of sterile sodium fumarate solution, 20.0mL of ster-
ile sodium-
L-lactate solution, 10.0mL of sterile Wolfe’s ... 1.5g
CoCl
2
·6H
2
O 190.0mg
MnCl
2
·4H
2
O 100.0mg
ZnCl
2
70.0mg
Na
2
MoO
4
·2H
2
O 36. 0mg
NiCl
2
·6H
2
O 24.0mg
H
3
BO
3
6. 0mg
CuCl
2
·2H
2
O 2.0mg
HCl (25% solution) 10....
Handbook of Microbiological Media, Fourth Edition part 65 ppsx
... liter:
NaNO
3
4 .66 7g
Na
2
SiO
3
·9H
2
O 3.0g
Sodium glycerophosphate 0 .66 7g
EDTA·2H
2
O 0.553g
H
3
BO
3
0.38g
Fe(NH
4
)
2
(SO
4
)
2
·6H
2
O 0.234g
MnSO
4
·4H
2
O 0.054g
FeCl
3
·6H
2
O 0.016g
ZnSO
4
·7H
2
O ... pH to 6. 8.
Artificial Sea Water:
Composition
per liter:
NaCl 23.477g
MgCl
2
·6H
2
O 4.981g
Na
2
SO
4
3.917g
CaCl
2
1.12g
KCl 66 4.0mg
NaHCO
3
192.0mg
H
3
BO
3
26. 0mg
SrCl...
Handbook of Microbiological Media, Fourth Edition part 78 ppsx
... toxic.
Preparation of Medium: To 65 .0mL of cooled, sterile base agar,
aseptically add 10.0mL of sterile yeast dialysate, 20.0mL of horse se-
rum, 2.0mL of sterile penicillin solution, and 1.0mL of sterile ... pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6. 6 6. 8. Filter sterilize.
Preparation of Medium: To 67 5.0mL of cooled, sterile Columbi...
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