... 10.0mL of CaCl2 solution, 10.0mL of MgSO4·7H2O solution,10.0mL of NaNO3 solution, 10.0mL of K2HPO4 solution, 10.0mL of KH2PO4 solution, and 10.0mL of NaCl solution to distilled/deionizedwater ... 1.0gPreparation of FeCl3 Solution: Add FeCl3 to distilled/deionizedwater and bring volume to 100.0mL. Mix thoroughly.Preparation of Bristol's Solution: Add 10.0mL of NaNO3 solu-tion, 10.0mL of ... psi pressure–121°C. Cool to50°–55°C. Preparation of Medium: Aseptically combine 500.0mL of solu-tion A, 1.0mL of solution B, and 500.0mL of solution C. Mix thor-oughly. Pour into sterile...