... 5NG,UKFax: +44 020 872 241 26Tel: +44 020 872 243 52E-mail: michelle.garrett@icr.ac.uk(Received 19 December 2007, revised 19March 2008, accepted 11 April 2008)doi:10.1111/j.1 742 -46 58.2008.0 646 3.xCyclin ... · 10111670 8 .4 · 107100 1(NH 4 )2SO 4 precipitation 2.9 · 1011596 4. 8 · 108205 5.7Butyl-sepharose 8.5 · 101016.2 5.3 · 10961 62.7Q-sepharose 9.6 · 1091. 24 7 .4 · 1096.8 88.8Superdex ... cellular phosphorylation of CDK4. TheABFig. 4. Src family kinases phosphorylate CDK4 on Y17 in vitro.(A) HeLa lysates (2.5 lg protein per well), and recombinant purifiedp49WEE1, C-YES and C-SRC...