... 5423 Design of expression vectors for RNA interference based on miRNAs and RNA splicing Guangwei Du, Joshua Yonekubo, Yue Zeng, Mary Osisami and Michael A. FrohmanDepartment of Pharmacology and the ... context: exon-ic miRNA in noncoding transcripts, intronic miRNAs in noncoding transcripts, and intronic miRNAs inprotein-coding transcripts [8,9]. Based on the accumulating knowledge on miRNAbiogenesis, ... precursors of microRNAs (miRNAs) driven by polymerase II promoters. We have designed newRNAi vectors, designated pSM155 and pSM30, that take into considera-tion miRNA processing and RNA splicing...