DNA REPAIR pptx

... H. pylori DNA repair in our laboratory was supported by NIH grant R21AI076569 and by the University of Georgia Foundation. DNA Repair 6 of DNA damage is not understood. No DNA repair genes, ... recombinational DNA repair. 3. Mechanisms of DNA recombinational repair known in model bacteria Although the bulk of DNA damage affects one strand of a duplex DNA segm...

Ngày tải lên: 28/06/2014, 15:20

... gắn với DNA mục tiêu chỉ khi có trình tự bổ sung  2) Lai không đặc hiệu: phản ứng lai xảy ra khi DNA mục tiêu có trình tự tương tự (không bổ sung hoàn toàn) Sự tương hợp giữa mẫu dò và DNA trên ... của một đoạn DNA đặc thù có mặt trong tế bào Thường sử dụng mẫu dò được đánh dấu huỳnh quang (Fluorescence in situ hybridization (FISH) hoặc động vị hoặc không đồng vị phóng xạ) DNA p...

Ngày tải lên: 22/12/2013, 15:16

... bound 32 P-labeled DNA (R) is the ratio of the fraction of bound 32 P-labeled DNA in the presence of the competitor DNA (cpm bound ⁄ cpm total ) to the fraction of bound 32 P-labeled DNA in the absence ... The lesions that escape repair can influence DNA replication, tran- scription, and the interaction of different proteins with DNA. All four B[a]P-N 2 -dG adducts inhibit DNA r...

Ngày tải lên: 19/02/2014, 00:20

... integrity and maintenance of DNA in all organisms including mitochondrial DNA (mtDNA). Mitochondrial DNA amounts to about 15% of the DNA content in Saccharomyces cerevisiae. A haploid cell contains about ... pathways of mtDNA maintenance have evolved between the two highly diver- gent yeast species. The role of Ydc2 for maintaining the content of mtDNA in fission yeast is not clear. Ap...

Ngày tải lên: 20/02/2014, 11:20

... of T4 DNA ligase comprises three steps and involves two covalent reaction intermedi- ates: E ỵ ATP $ EAMP ỵ PP i 1ị E AMP ỵ ndsDNA $ EAMPndsDNA ! E ỵ AMPndsDNA 2ị E ỵ AMP ndsDNA $ E AMPndsDNA ! ... EAMPndsDNA ! E ỵ AMPndsDNA 2ị E ỵ AMP ndsDNA $ E AMPndsDNA ! E ỵ dsDNA ỵ AMP 3ị where ndsDNA is nicked dsDNA, AMPndsDNA is ndsDNA adenylated at the 5Â-phosphate of the nick, and a one-sided arrow...

Ngày tải lên: 21/02/2014, 00:20

... BL21(DE3) and expressed and purified as described for CYIIB. Purification of promoter DNA fragments The promoter DNA templates AdML and AdE4 in pGEM vector [18] were transformed into E. coli strain ... plasmid Giga kit (Qiagen). The plasmid DNA was cut by BamHI and EcoRI, phenol/chloroform extracted and precipitated. After washing with 70% ethanol, the DNA pellet was dissolved in buffer...

Ngày tải lên: 07/03/2014, 15:20

... transferase. Keywords: deoxyribose phosphate lyase; DNA polymerase; DNA repair enzyme; terminal deoxyribonucleotidyl trans- ferase. Ten years ago, only five DNA polymerase species (a to e) were known, ... Blanco, L. (2002) DNA polymerase k, a novel DNA repair enzyme in human cells. J. Biol. Chem. 277, 13184–13191. 21. Ramadan, K., Shevelev, V., Maga, G. & Hubscher, U. (2002)...

Ngày tải lên: 07/03/2014, 15:20

... in postnatal growth, suggesting that mHR23B may have functions beyond those related to XPC and DNA repair [19]. Here, we report the three-dimensional solution struc- ture of the XPCB–hHR23B (275–342) ... cerevisiae nucleotide excision repair gene RAD23. Genomics 31, 20–27. 8 You JS, Wang M & Lee SH (2003) Biochemical analysis of the damage recognition process in nucleotide excision...

Ngày tải lên: 07/03/2014, 17:20

... various DNA repair mechanisms, the repair of a specific DNA damage is often conserved from bacteria to human, and in many cases the proteins are highly similar [5]. Little is known about DNA repair mechanisms ... gondii DNA repair enzyme; RRM, RNA recognition motif; HCA, hydrophobic cluster analysis; MMC, DNA- damaging agent mitomycin C; G-patch, glycine rich motif; SF45, spli...

Ngày tải lên: 08/03/2014, 22:20

... Phát hiện đột biến nhờ Chip ADN (DNA chip) Các oligonucleotide được đặt trên con chip, sau đó cho tiếp xúc với ADN được gắn

Ngày tải lên: 12/03/2014, 06:20