... he re-cloning of the enzymegene into the Nco I-HindIII site of pATE19, the secondamino acid of the enzyme was changed from arginine toglycine. The plasmid pBASPR was introduced into E. colicells. ... both enantiomers of glutamate,asparagines, glutamine, alanine, serine, lysine, and arginine,were inactive as substrates. The enzyme was not inhibitedby these nonsubstrate amino acids (10 mM), ... unit of the enzyme was defined as theamount of enzyme that catalyzes the formation of 1 lmol of D-aspartate fromL-aspartate p er hour.Protein concentrations were determined using a proteinassay...